Microbiology Lab Chap 1

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Observing Bacteria and Blood
Cynthia Alonzo, M.S. Version 42-0249-00-01

Lab Report Assistant This document is not meant to be a substitute for a formal laboratory report. The Lab Report Assistant is simply a summary of the experiment’s questions, diagrams if needed, and data tables that should be addressed in a formal lab report. The intent is to facilitate students’ writing of lab reports by providing this information in an editable file which can be sent to an instructor.

Exercise 1: Viewing Prepared Slides
Questions A. Identify the following parts of the microscope and describe the function of each.

A=Ocular Lens- Magnifies the image as it brings it to your eye (Alonzo p52). B= Body Tube- Connects to the arm of the microscope with a gear that allows you to focus the image to focus the image when changing lenses or observers and moving the lenses away from the stage when changing specimens (Alonzo p53). C= Condenser- A Lens system that aligns and focuses the light from the lamp onto the specimen (Alonzo p53) D= Objective Lens- Gathers light from the specimen (Alonzo p53). E= Stage- where the specimen is placed (Alonzo p52).

F= Diaphragm in the light path to alter the amount of light that reaches the condenser (Alonzo p52) G= Lamp- Produces Light (Alonzo p52).
H= Course Focus Knob- Brings the object into the focal plane of the objective lens (Alonzo p53). I= Fine focus Knob- Makes fine adjustments to focus the image better (Alonzo p53). J= Arm- The curved portion that holds the microscopes optical parts in place and keeps them aligned (Alonzo p53). K= Clip- Holds the specimen secure on the stage (Alonzo p52). L= Base –Supports the weight of the microscope (Alonzo p53).

Define the following microscopy terms:

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