EFFECT OF USED OIL IN Trichoderma harzianum
A Thesis Presented to the
Faculty, Special Science Class
Iloilo National High School
La Paz, Iloilo City
In the Partial Fulfillment
in the subject
Sheree Joie Montomo Polonan
Introduction of the Study
Chapter 1 is divided into five parts: (1) Background of the Study, (2) Statement of the Problem and Hypothesis, (3) Significance of the Study, (4) Definition of Terms and (5) Delimitation of the Study.
Part one, Background and Theoretical Framework of the Study presents the introduction of the study, discusses the reasons for choosing the study and its Theoretical Framework. Part two, Statement of the Problem and Hypothesis, describes the purpose in conducting the study and enumerates the specific objectives of the study. Part three, Significance of the Study, states the benefits that could be derived from the findings of the study. Part four, Definition of Terms, lists alphabetically the research terminologies for purpose of clarity and understanding. Part five, Delimitation of the Study, identifies the average of the investigation, in terms of the treatments to be used and the materials to be used in the study.
Background of the Study
According to Harman (2007), Trichoderma spp. are most prevalent culturable fungi that are present in soils and diverse habitats. They are suited to remediate oil-polluted environments. Specific fungus possesses biodegration potential to used oil like crude oil (Obire and Putheti, 2009). Morgan (2011), Trichoderma has been shown to establish and proliferate on a range of soilless substrates, and colonization is greater on a growing medium. As noted by Obire and Putheti (2009), Fungi follow a pattern of growth and development which is less predictable than for plants and animals. They do not become fixed into a developmental pathway. All cells of a hypha may initiate a colony. Because as noted by Lepp (2012), hyphal species like the fungi Trichoderma harzianum, the individual hyphae in a mycelium are not more than a hundredth of a millimeter in a diameter.The specific growth rate of the hyphae in the colony’s peripheral growth zone is to be identical to the organism’s maximum specific growth rate in submerged culture (Trinci, 1971). Hyphal growth need not to be random, since fungi respond to chemical signals(Lepp and Fagg, 2012) since Trichoderma species are known for the production of lytic enzymes and antibiotics(Harman, 2007). In this study the effect of used oil, specifically, crude oil in Trichoderma harzianum will be determined. Radial growth of hyphae, spore counting, rate of spore formation will be determined through agar-cup/agar-well diffusion method.
Figure 1. Depicts the graphical framework of the study.
Statement of the Problem and Hypothesis
This study will be conducted to determine the effect of used oil in the growth of Trichoderma harzianum. Specifically, this study sought answers to the following questions:
1 . What is the radial growth, spore count and spore formation of Trichoderma harzianum treated with different amounts of crude oil compared to the controls (+ & - )? 2. What is the morphological appearance of Trichoderma harzianum treated with different amounts of crude oil? 3. Is there a significant difference in the radial growth, spore count and spore formation of Trichoderma harzianum treated with different amounts of crude oil? In view of the preceding questions, these hypothesis were advanced: 1 . There is no significant difference in the radial growth, spore count and spore formation of Trichoderma harzianum treated with different amounts of crude oil. Significance of the Study
The study will hopefully be beneficial...
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