Gram Staining
A Detailed Lesson Plan
I. OBJECTIVES:
Given a set of activities, the students should be able to attain at least 75% proficiency and should be able to:
A. describe staining;
B. enumerate the common stains;
C. differentiate between an acidic dye and a basic dye;
D. compare simple, differential and special stains; and
E. list the steps in preparing a Gram stain and describe the appearance of a gram-positive and gram- negative cells after each step.\
II. SUBJECT MATTER: Biological Techniques Topic: Staining A. Materials:
* cartolina- used during the activity in lesson proper(flashcards) * iodine- used in the activity in application * glass slide- used in the activity in application(wet mount) * cover glass- used in the activity in application(wet mount) * ball of cloth- used in the activity in motivation * manila paper- used in the activity in lesson proper(table) * improvised canal- used in the activity in motivation * mud- used in the activity in motivation
B. References:
Tortora, Geral J., Funke, Berdell R., and Case, Christine L., “Microbiology (An Introduction) fifth edition”, the Benjamin/ Cummings Publishing Company, Inc., pages 61-66. Internet websites: Bruckner, Monica Z.,” Basic Cellular Staining.” serc.carleton.edu, April 14, 2013 http://serc.carleton.edu/microbelife/research_methods/microscopy/cellstain.html ANTRANIK,” Stain uses, types and applications.”antranik.org, March 11, 2012 http://antranik.org/stain-uses-types-and-applications/ Staining Techniques, from http://www.cliffsnotes.com/study_guide/StainingTechniques.topicArticleId-8524,articleId-8413.html Bismarck Brown,May 2005, from http://stainsfile.info/StainsFile/dyes/21000.htm Mifflin,Houghton.,”Papanicolaoustain.”http://medicaldictionary.thefreedictionary.cHYPERLINK "http://medical-dictionary.thefreedictionary.com/"om,2007 from
I. OBJECTIVES:
Given a set of activities, the students should be able to attain at least 75% proficiency and should be able to:
A. describe staining;
B. enumerate the common stains;
C. differentiate between an acidic dye and a basic dye;
D. compare simple, differential and special stains; and
E. list the steps in preparing a Gram stain and describe the appearance of a gram-positive and gram- negative cells after each step.\
II. SUBJECT MATTER: Biological Techniques Topic: Staining A. Materials:
* cartolina- used during the activity in lesson proper(flashcards) * iodine- used in the activity in application * glass slide- used in the activity in application(wet mount) * cover glass- used in the activity in application(wet mount) * ball of cloth- used in the activity in motivation * manila paper- used in the activity in lesson proper(table) * improvised canal- used in the activity in motivation * mud- used in the activity in motivation
B. References:
Tortora, Geral J., Funke, Berdell R., and Case, Christine L., “Microbiology (An Introduction) fifth edition”, the Benjamin/ Cummings Publishing Company, Inc., pages 61-66. Internet websites: Bruckner, Monica Z.,” Basic Cellular Staining.” serc.carleton.edu, April 14, 2013 http://serc.carleton.edu/microbelife/research_methods/microscopy/cellstain.html ANTRANIK,” Stain uses, types and applications.”antranik.org, March 11, 2012 http://antranik.org/stain-uses-types-and-applications/ Staining Techniques, from http://www.cliffsnotes.com/study_guide/StainingTechniques.topicArticleId-8524,articleId-8413.html Bismarck Brown,May 2005, from http://stainsfile.info/StainsFile/dyes/21000.htm Mifflin,Houghton.,”Papanicolaoustain.”http://medicaldictionary.thefreedictionary.cHYPERLINK "http://medical-dictionary.thefreedictionary.com/"om,2007 from
References: Internet websites: Bruckner, Monica Z.,” Basic Cellular Staining.” serc.carleton.edu, April 14, 2013 http://serc.carleton.edu/microbelife/research_methods/microscopy/cellstain.html