Biotechniques(Staining)

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A Detailed Lesson Plan

I. OBJECTIVES:

Given a set of activities, the students should be able to attain at least 75% proficiency and should be able to:

A. describe staining;

B. enumerate the common stains;

C. differentiate between an acidic dye and a basic dye;

D. compare simple, differential and special stains; and

E. list the steps in preparing a Gram stain and describe the appearance of a gram-positive and gram- negative cells after each step.\

II. SUBJECT MATTER: Biological Techniques
Topic: Staining
A. Materials:

* cartolina- used during the activity in lesson proper(flashcards) * iodine- used in the activity in application
* glass slide- used in the activity in application(wet mount) * cover glass- used in the activity in application(wet mount) * ball of cloth- used in the activity in motivation

* manila paper- used in the activity in lesson proper(table) * improvised canal- used in the activity in motivation * mud- used in the activity in motivation

B. References:

Tortora, Geral J., Funke, Berdell R., and Case, Christine L., “Microbiology (An Introduction) fifth edition”, the Benjamin/ Cummings Publishing Company, Inc., pages 61-66. Internet websites:

Bruckner, Monica Z.,” Basic Cellular Staining.” serc.carleton.edu, April 14, 2013 http://serc.carleton.edu/microbelife/research_methods/microscopy/cellstain.html ANTRANIK,” Stain uses, types and applications.”antranik.org, March 11, 2012 http://antranik.org/stain-uses-types-and-applications/ Staining Techniques, from http://www.cliffsnotes.com/study_guide/StainingTechniques.topicArticleId-8524,articleId-8413.html Bismarck Brown,May 2005, from http://stainsfile.info/StainsFile/dyes/21000.htm Mifflin,Houghton.,”Papanicolaoustain.”http://medicaldictionary.thefreedictionary.cHYPERLINK "http://medical-dictionary.thefreedictionary.com/"om,2007 from http://medical-dictionary.thefreedictionary.com/Papanicolaou+stain Abedon,StephenT.,”MicroscopyandStaining.”mansfield.ohiostate.edu,3/28/2003 from http://www.mansfield.ohiostate.edu/~sabedon/black03.htm

III. PROCEDURE
A. Motivation
|Teacher’s Activity |Student’s Activity | |Good afternoon class! |Good afternoon sir! | | | | |We will play a game .I will give you a ball of cloth. | | |All you have to do is to roll the ball of cloth into the improvised | | |canal with mud. | | |Who wants to volunteer? | | | |(students will raise their hands) | |What did you observe when you rolled the ball of cloth into the | | |improvised canal with mud? Yes Mark. | | | | | |That’s right! After you observed that the ball of cloth got stained, |The ball of cloth got stained....
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