The purpose of this lab is to find the amount of blue dye #1 in the blueberry pomegranate gatorade.…
In our experiment, there was a source of error because in the Concentration vs. Absorbance graph’s line of best fit, there was a y-intercept, but there isn’t suppose to be one since the concentration is 0, the absorbance should be 0. There are no particles in the solution, distilled water, so nothing should be absorbed. This error could have been caused by the leaving the diluted solution out too long, so the water in the solution could have evaporated, leaving only the blue dye. It is not a diluted solution if there is just dye left in the beaker because it would go be a higher concentration than the actual solution’s concentration…
The use of red dye #40 is common in various soft drinks today. The labels on these beverages do not specify how much dye we are consuming. We did this experiment to find out which soda uses the most dye. Using a spectrophotometer, we measured how much light is absorbed by various known concentrations of red dye. After collecting this data, a standard curve was made that correlated the concentration of red dye #40 to its absorbance rate. Our results showed that the sample of strawberry crush had the highest concentration of red dye #40 when compared to grape soda, diet cherry, and big red. After doing this experiment, one knows how much red dye #40 he or she is consuming when drinking these products.…
Spectrophotometry was used in the lab to determine whether non-magnetized zeolite, magnetized zeolite, or charcoal was the more effective sequestration agent for Procion Red Dye. A calibration curve was created with the known concentrations and the absorbances of the Procion Red Dye dilutions at λmax. The slope of the calibration curve was used to determine the concentration of the analytes. As a result, charcoal was shown to be the more effective sequestration agent.…
Purpose: To determine the concentration of dye in a sports drink using spectroscopy and graphical analysis. We will be using beers law to find the unknown concentration of dye in a sports drink.…
To examine the relationship between color of a solution and the light it absorbs. When it comes down to it our eyes do a accurate job of depicting and distinguishing colors. The purpose of this experiment is to take that a little farther, become more precise. With the use of a spectrophotometer one can determine the wavelength and intensity of the light absorbed. By adding a light source to the solution we can determine the spectrum of that solution, or in other terms the light absorbed. Absorbance is a key part of the experiment, especially the relationship between absorbance and concentration. In other terms, the higher the absorbance, the deeper the color; also the more light absorbed, the less light that passes through. Now when it comes to combining colors, there’s a discrepancy between the color light spectrum, and the painters color spectrum. Since being a child you know that combining two colors would give you one solid color. The same falls true in chemistry, when you combine two chemicals you’ll get the respected outcome, for example, blue and red will make purple. Now in the experiment when you add Erioglaucine and Allura Red, the color emitted is the same as it would be with Crystal Violet. In reality they’re both purple, but when you take closer look at it the color spectrum for Crystal Violet is different then that of the combination to make purple. When you look at the combination of Allura Red and Erioglaucine you see a combination of the red and blue color spectrum. So as far as the color wheel goes, to the eye they’re the same, but when you mix light, you combine the color spectrums, creating a different one than as if it was just the primary color. Beer’s Law shows the relationship for absorbance of a material in which light is passing: A=ebc ( A being absorbance, e being molar absorptivity , b being path of the length of the sample, and c…
Discussion of Theory: Chromatography, resolution and selectivity played major roles in making the experiment work. The experiment demonstrated a common use of chromatography, and works to explain a way to separate mixtures. The dyes were successfully separated and analyzed in the cartridge.…
The purpose of this experiment was to observe the color change and the concentration to determine the…
This investigation used spectroscopy to evaluate light absorption in different solutions. A spectrophotometer was used in the lab to determine these values. A spectrophotometer is an apparatus used to “measure the absorption of radiation in the visible and UV regions of the spectrum and allows precise at a particular wave length” (Jones et al., 2007). The amount of light absorbed by a substance is directly in relation to the concentration of the solute and also the wavelength moving through the solute (Jones et al., 2007). This is commonly referred to as Beer’s Law and can be expressed as A= εl [C]. Beer’s Law equation measures the absorbency of light, making it an effective measure as spectrophotometers give exact values for absorbency (Jones et al., 2007).…
The purpose of this experiment is to apply Beer’s Law by analyzing samples provided by Q laboratory to determine their absorbance and prepare a Beer’s law plot. Molarity of these samples was also calculated to determine concentration and percent error rate. Students also analyzed the concentration of blue dye #1 to determine the concentration of blue dye #1 in a commercial blue dye drink.…
Different amounts of FD&C Blue I were diluted with water to make eight differently concentrated 10 mL solutions. Samples were placed in the spectrophotometer to determine the percent transmittance of FD&C Blue 1. All of the data was summarized in graphs to predict the concentration of FD&C Blue I in a sample of Gatorade.…
Abstract: This experiment helps determine the rate of reaction of crystal violet while it reacts with sodium hydroxide with respect to crystal violet. The amount of sodium hydroxide is varied in this experiment while crystal violet is kept at a constant. The transmittance of crystal violet is observed and recorded using a colorimeter and the data obtained is used to plot graphs which are manipulated using LoggerPro software to produce the desired outcome; rate of reaction of crystal violet. Upon completion of the experiment it was seen that the rate of reaction of crystal violet turned out to be 1 which meant the reaction was first order with respect to crystal violet. This was deduced upon plotting the graph of ln Absorbance versus time of crystal violet and by drawing the line of best fit, which showed that the slope graph was 1 which is the rate of reaction. This whole experiment was based upon the equation: Rate= k [CV+] [OH-], where k stands for the rate constant.…
One of the most cliche statements that has ever been said is “People that you are surrounded with will make a big influence on you.” People always have a big influence on things especially other people. This connection is relevant to the characters in Make Lemonade by Virginia Euwer Wolff. The main character, LaVaughn is a young teen, whose mom is always on her back about having a good future and going to college. Which is completely opposite to Jolly, who is a teen mom with two kids and is learning how to survive life with only her and her kids. Throughout the novel, both LaVaughn and Jolly learn from each other and their differences in their lives. These challenges and experiences in life help the characters develop a new outlook on life.…
The objectives of this experiment are to understand why and how spectroscopy is used to determine the components and concentrations of a solution, describe various types of spectroscopy, describe the visible and ultraviolet regions of the electromagnetic spectrum, define Beer’s law and define the relationship between absorbance and transmittance. Other learning objectives are to create a Beer’s law plot for a series of samples with known concentrations, collect spectrophotomic data from unknown and known FDC blue dye samples, perform serial dilutions, calculate concentrations, perform linear regression and determine the equation of a best fit line.…
Here at Luscious Lemonade our mission is to make our customers happy and satisfy their taste for lemonade. Our community and customers always come first.…