Laboratory Protocol for Carbohydrates

Topics: Glucose, Iodine, Carbohydrate Pages: 3 (670 words) Published: August 27, 2013
Experiment No. 7
HYDROLYSIS AND ANALYSIS OF CARBOHYDRATES

Methodology

Materials:
|1% solutions: | conc. H2SO4 | |glucose, fructose, maltose, sucrose, lactose, |Molisch reagent | |agar-agar, gum arabic,glycogen, cotton, |I2 in KI solution (Lugol’s iodine reagent) | |starch |Benedict’s reagent | |10% HOAc solution, |Barfoed’s reagent | |conc. HCl |Orcinol reagent | |solid Na2CO3 |conc. HNO3 | |dilute NaOH | |

Equipment:
hot plate

Procedure:
A. Hydrolysis
1. For 1% sucrose, lactose and maltose solutions
a. Measure out 5 ml 1% solution in a small beaker.
b. Add 3 drops conc. HCl and boil for 5 minutes.
c. Cool & neutralize with dilute NaOH. Set aside for part B. 2. For 1% starch, agar-agar, gum arabic, glycogen, cotton and starch a. Measure out 5 ml 1% solution in a small beaker.

b. Add 5 drops conc. HCl.
c. Cover the mouth of the test tube with a marble and boil in a water bath for 30 minutes. d. Neutralize with solid Na2CO3. Set aside for part B. 3. For polysaccharide extract

a. Repeat procedure...
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