Purpose/Objective: The purpose/objective of this lab was to see if environmental conditions changed the speed of the reaction. The catalyst being used was the spark (the match). The Chemical reaction being tested was how fast the hydrogen peroxide could turn into water and oxygen. The theory that was being tested was; Enzymes speed up reactions. The facts that I wanted to determine were matches making the chemical reaction happen quicker or not. Materials:
-Glass Stirring Rod
-Hydrochloric Acid -Hydrogen Peroxide
1) Put on safety goggles
2) Get test tubes
3) Put chicken liver in 5 of 6 test tubes, an sand in all of the test tubes 4) Get beaker of Hydrogen peroxide with a dropper
5) Place all 6 test tubes in the empty beaker
6) Fill test tube #1 with sand and a piece of liver. Use the glass stirring rod to crush the liver and sand together. 7) Fill test tube #2 with sand but DO NOT crush the piece of liver. 8) Fill test tube #3 with a piece of liver and sand. Then place this test tube into a breaker of ice. Set this beaker aside for as long as possible. 9) Fill test tube #4 with sand and a piece of liver. Then add 1mL of hydrochloric acid into the test tube and put it aside for as long as possible. 10) Fill test tube #5 with sand and a piece of liver. Then add 1mL of Hydrogen Peroxide. 11) For test tube #6, put in a pinch of sand and leave it that way. 12) Record observations
13) When finished, keep goggles on, clean out test tubes, return them to the test tube holder, put away your goggles once everyone is finished, and then return to your seat quietly.
1) What is an Enzyme? What are their roles in chemical reactions? A: An enzyme is a catalyst and it either starts, or speeds up a chemical reaction by lowering the activation energy without being changed or used up.
2) Describe what happened to test tube 2 when hydrogen peroxide was added to the liver. What gas was produced? What liquid was left? A: When the Hydrogen Peroxide was added to the liver, it started to bubble and yellow liquid was left. The gas that was produced was oxygen and the liquid left behind was water.
3) What factors could have influenced the rating of the chemical reaction between the liver and the hydrogen peroxide? Give two factors. Explain each. A: The reaction in test tube 2 was exothermic because the test tube got hotter and energy was released.
4) What factors could have influenced the rating of the chemical reaction between the liver and the hydrogen peroxide? Give two factors. Explain each. A: The two factors that could have influenced the rating of the chemical reaction were the bases and acids and the temperature. The bases and acids could have influenced the rating because they change the pH. The temperature could have influenced the rating because lower temperatures tend to slow down reactions while higher temperatures usually speed them up.
5) What effect does temperature have on the activity level on an enzyme? A: Temperature has an effect on the activity level on an enzyme because heat can raise the rate of reaction. On the other hand, by making the environment colder, it will slow down the reaction down.
6) Is there a control group in this experiment? If so which tube served as a control? A: The control group was test tube 6, containing only sand. This is because every test tube had sand and liver in it but test tube #6, this allowed one variable to be tested.
7) Why does the glowing splint test light up?
A: The glowing splint test lights up because when the match hit the hydrogen peroxide, it caused a reaction which caused the splint to light up.
8) What was the purpose of adding sand to the test tubes?
A: The purpose of adding sand to the test tubes was so that the sand could cut the liver up and release catalase, which are the enzymes...
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