Lab Report Microscope

Topics: Lens, Microscope, Optics Pages: 15 (1763 words) Published: January 30, 2015


The Microscope Lab

Brandy Talley

PRCC Biology Lab Online

June 13, 2013

Purpose:

The purpose of this lab is to learn about different types of microscopes that we use today, learn the parts of the microscope, and how to properly use a microscope by exploring various slides.

Materials:

*Microscope
*Letter “e” slide
*Onion root slide
*Bacterial capsule slide
*Cheek smear slide

Lab Notes:

1.What is the difference between a compound microscope and a stereo microscope? Be detailed.

Compound microscopes and stereo microscopes are different in a few ways. The compound microscope can provide a magnification up to 1000X. It is better suited to view smaller objects such as animal tissue and thin pieces of plants. The stereo microscope on the other hand is able to view much larger objects such as rocks, insects, and flowers. However, the stereo microsope has a much lower magnification range of only.

2.If the ocular of your microscope is 10x and your objective that you are viewing your specimen under is 10x, what is the total magnification?

To find the total magnification, you will multiply the ocular of your microscope by your objective. This results in the total magnification being 100x.

3.How does the light change on the compound microscope?

Light on the compound changes from adjusting the iris diaphragm

4.What is the magnification of the ocular?

The magnification of the ocular is 10x.

5.Which type of microscope could you view a Frog?

A stereo microscope would allow a person to view a frog.

6.What is the circular area that you see when you look under the microscope?

The circular area you see when you look under the microscope is called the field of view.

7.What happens to the field of view as you go from low to high power on a compound microscope?

When you go from low to high power on the compound microscope the field of view decreases.

8.What happened to the light as you go from low to high power on the compound microscope?

The amount of light decreases as you go from low to high power on the compound microscope.

9.What power do you always begin on when you start looking at something on the microscope?

Always begin with low power when you start looking at something under the microscope.

10.When you get done using the microscope, what power do you always put the microscope

Always put the microscope back to low power after you are done using the microscope.

Procedures:

1. Turn the lights on
2. Rheostat to 10
3. Place slide letter “e” on stage
4. Secure it
5. Switch to 4x view
6. Center specimen
7. Switch views
8. Adjust oculars to 70
9. Adjust course focus to until slide is in view
10. Adjust iris diaphragm
11. Adjust fine focus
12. Center image
13. View under 40x
14. Screen shot
15. switch view to 100x
16. screen shot
17. switch to 400x
18. adjust iris diaphragm
19. adjust fine focus
20. screen shot
21. switch to 1000x
22. adjust diaphragm
23. screen shot
24. switch view
25. return to low power
26. select onion root tip slide
27. center slide
28. scroll to top position
29. switch view
30. view in 40x
31. adjust iris diaphragm
32. adjust fine focus
33. screen shot
34. view in 100x
35. adjust diaphragm
36. adjust fine focus
37. screen shot
38. switch view
39. adjust iris diaphragm
40. screen shot
41. switch to 1000x
42. adjust diaphragm
43. screen shot
44. return to low power
45. select bacterial capsule slide
46. center slide
47. move stage to top position
48. switch view
49. adjust course focus
50. adjust iris diaphragm
51. adjust fine focus
52. screen shot
53. switch view to 100x
54. screen shot switch to 400x
55. adjust iris diaphragm
56. screen shot
57. switch to 100x
58....
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