Factors that Affect the Rate of Reaction of Peroxidase Purpose: To determine the effect of various factors on the rate of reaction between an enzyme and its substrate‚ and also to determine the optimal ranges under which the enzyme activity is maximized. Also to determine whether saline and alcohol are inhibitors or activators Hypothesis: PH factor prediction: I predict that as the pH increases so the activity of the enzyme will increase until it reaches optimum pH range (pH 7) because the
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show how the amount of substrate affects the rate of the reaction. Hypothesis: In this experiment I think the amount of substrate will simply increase the reaction. As I increase the surface area of the potato the gas given off from the reaction will increase. Therefore to sum things up‚ my hypothesis is when the Independent variable increases so will the dependant variable. Independent variable: - Surface area of potato Dependant variable: - Gas Controlled variable: - Equal size of potatoes
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Chemical reactions can happen slowly or fast. The speed of the reaction rate can be changed. Some of the factors that can affect the reaction rate are surface area‚ temperature‚ and concentration and pressure. Surface area is the exposed‚ outer layer of a solid. Increased surface area increases reaction rate because more particles on the surface of a solid come in contact with the particles of another substance. For example‚ if you place a donut stick into water‚ the donut will react slowly with
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the substances used in respiration (sugar or oxygen) and the waste products created. The breakdown of glucose also releases carbon atoms‚ which become available for biosynthetic reactions‚ enabling the yeast to grow and reproduce by budding or fission. The rest of the carbon ends up in the by-products of these reactions‚ such as carbon dioxide‚ ethanol‚ and other more minor
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Effect of Temperature on Peroxidase Ability to Break Down H2O2 By: Rodneika Crutcher Abstract Temperature affects the ability of peroxidase to break down hydrogen peroxide. In this experiment our professor extracted peroxidase from potato tissue. In order to determine how temperature affects peroxidase we created solutions and measured their absorbance levels after water bath treatments. The more absorbent the solution was the less hydrogen peroxide there was in the solution. This means the peroxidase
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changes in temperature expands the air inside of the basketball causing it to bounce higher. This experiment was decided by observations made while playing the sport basketball. It was observed that when a basketball is left in the sun and made warm‚ the ball bounces higher when dribbled than if left inside. Our assumed result was that an increase in temperature would cause an increase in air pressure causing the bounce of the basketball to increase. The independent variable was the temperature in which
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Enzyme Reaction Rates Under Different Conditions Introduction In this lab you will observe how the rate of a reaction is changed as certain factors are changed. You will observe how changing factors like temperature‚ pH‚ substrate concentration‚ and enzyme concentration changes the rate of an enzymatic reaction. In this experiment you will act as an enzyme by breaking toothpicks witch act as substrates. After observing the results of this experiment you will be able to determine what causes the
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1‚ 2014 Tyrosinase Enzyme Lab Question What are enzymes and how can we cause changes in enzyme function? Objective The objective of this experiment is to observe if changes in temperature will cause a disruption in enzyme function. Hypothesis If we add and boil enzyme in L-Dopa‚ then the color will become darker because the temperature will denature some of the enzymes. Variables Independent: Temperature Dependent: Color change Control: Time‚ Room temperature Jobs We were assigned
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graph‚ you can see that there is a relation between the rate of reaction and the surface area. The larger the surface are‚ the faster the rate of reaction seems to be. However‚ although the grain with the largest surface area (the smallest grain) reached the highest point within the shortest amount of time‚ its end result was still lower then the medium sized grain. The explanation for this result is relatively easy. The rate of a chemical reaction can be increased by increasing the size of the surface
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Effect of Temperature on Enzyme Activity Almost all chemical reactions that occur in living organisms are catalyzed by enzymes. Many factors in a cell’s environment affect the action of an enzyme. In this investigation‚ you will design an experiment to determine the effect of temperature on an enzyme-catalyzed reaction. You will complete an entire lab write-up using the CHS LAB REPORT FORMAT. Fundamental Question How does temperature affect the rate of an enzyme-catalyzed reaction? Materials
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