Isolation of Pure Cultures by Dilution Techniques and Gram Staining Method Results Table 1. Gram stain reaction and cellular features of the culture. Gram staining methods were applied on the given mixture of Bacillus cereus‚ Escherichia coli and Staphylococcus aureus and then examined microscopically. Results were recorded in Table 1. Gram Reactions Cell shapes Cell Ends and Arrangement Size Distinctive Characters Predicted Bacteria Bacteria 1 Gram positive (purple) Cocci Rounded‚ clusters
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Many lithotrophs‚ such as the nitrifying bacteria‚ also have long generation times. Some bacteria that are pathogens‚ such as Mycobacterium tuberculosis and Treponema pallidum‚ have especially long generation times‚ and this is thought to be an advantage in their virulence. Generation times for a few bacteria are are shown in Table 2. Table 2. Generation times for some common bacteria under optimal conditions of growth. |Bacterium |Medium
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Endospore and Capsule Cell Staining Allison Lui Partner: Mary Chagin BIOL-235 W07 Dr. Runco 2/10/15 Introduction The purpose of this lab is to learn what endospore and capsules are‚ and how to identify them under a microscope using capsule and endospore staining methods. Capsules are found only in select bacteria‚ and serve a protective purpose. Made out of sugars and proteins‚ they are antiphagocytic‚ which prevents other cells to engulf the bacteria through phagocytosis. It
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The methylene blue staining procedure is used to measure yeast viability based on the assumption that the methylene blue will enter the cells and be broken down by living yeast cells that produce the enzymes which breaks down methylene blue‚ leaving the cells colourless. The non- viable cells do not produce this enzyme (or enzymes) and as such the methylene blue that enters the cells are undegraded causing the cells to remain coloured (the oxidized form concentrates intracellularly). The coloured
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Bacteria Classification By Gram Staining THE AMERICAN UNIVERSITY IN CAIRO BIOLOGY DEPARTMENT SCIENCE 453 : BIOLOGY FOR ENGINEERS REPORT No.1 Presented By : Karim A. Zaklama 92-1509 Sci. 453-01 24/2/96 Objective: To test a sample of laboratory prepared bacteria and categorise it according to Christian’s gram positive and gram negative classes and also by viewing it under a high powered microscope and oil immersions; classify its shape and note any special characteristics. Introduction: Bacteria
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Cellular and Molecular Techniques - Lab #1 BIO 349 Lab Report #1 Microscopy and Staining Abstract The primary focus of this lab was on microscopy and simple stains. Microscopy that was used were magnification‚ slide preparation‚ and staining. Methylene blue‚ a simple staining component‚ was used to stain the slide in order to see the different microbes and determine their cellular shape. Introduction The purpose of this lab was to become familiar with the light microscope and how
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1. What are the advantages and disadvantages of the different types of light and electron microscopes discussed in Chapter 3 that are used to study microorganisms? Focus your response in terms of the following parameters The disadvantage of using a light microscope is its inability to magnify a specimen as great as an electron microscope. An electron microscope can magnify up to 500‚000 times‚ whereas‚ a light microscope can only magnify an object up to 2000 times. The resolving power or resolving
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belts‚ caps‚ etc‚ are the most preferred clothing for today’s youth. This study discusses compositions and methods of reducing or preventing back staining of indigo dyes on the weft yarn and pocket of denim garments. The dye‚ as it is removed from the denim material‚ post treatment with cellulase or by a conventional washing process may cause ‘back staining’ or ‘redeposition’ on the denim material; e.g : re-coloration of blue threads and blue coloration of white threads‚ resulting in less contrast between
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Method • Methodology is performed through 3 successive days: First Day: Trypan Blue cell viability assay: Description Trypan blue staining is a simple way to evaluate cell membrane integrity (and thus assume cell proliferation or death) but the method is not sensitive and cannot be adapted for high through put screening. Short 96 well assay: EACH condition should be done in triplicate or more. Steps 1. Trypsinize one T-25 flask and add 5 ml of complete media to trypsinized cells. Centrifuge
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Lab 5 –Cell Structure and Staining using Microscopy Instructions: Please download this MSWord document to your computer and answer the questions as asked. Then save the document and upload it to Bb using the Assignment feature provided. This assignment is worth a total of 100 points – there are 20 questions worth 5 points each. NAME Buket Rembert In Lab 3 you were introduced to microscopy. In this lab you will be adding to that experience by reviewing the differences in cell structure for Prokaryotes
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