work best in killing the harmful bacterial strain‚ Bacillus subtilis. KB testing or disc diffusion antibiotic sensitivity testing is measured through the diameter in millimeters to find how resistant the antibiotic to the bacteria. The hypothesis of Windex fell correctly with my results but Lysol was only 4mm more sustainable then Windex. Lysol with the biggest zone of inhibition of 28mm allowing this disinfectant to kill the most Bacillus subtilis bacteria. Still‚ Lysol was succeeded to work‚ but
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Antibiotics Vs Tea Tree Oil To determine the effect of various different antibiotics and Tea Tree oil on the Bacillus subtillis different types of bacteria. I will test six antibiotics and the Tea Tree oil on Bacillus subtilis. I will test tetracycline‚ chloramphenicol‚ ampicillin‚ sulphatriad‚ penicillin G and Streptomycin. I would also like to see if Bacillus subtillis shows resistance to Antibiotics or to Tea Tree oil. The use of penicillin is being reduced because of the resistance
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ISOLATION AND SCREENING EXTRACTED FROM GOTU KOLA (Centella asiatica) ROOTS EXTRACT Submitted by: ALIANA CLARENCE B. JAMERO IV – REG A Research Paper Presented to the Faculty of Gusa Regional Science High School Region X In Partial fulfilment of the requirements For Research 2 Submitted to: ERMA S. DAPIN Adviser March 2014 APPROVAL SHEET The research paper hereto attached‚ entitled; “ISOLATION AND SCREENING EXTRACTED FROM GOTU KOLA (Centella asiatica) ROOTS EXTRACT”
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Research in Biotechnology‚ 3(6): 51-58‚ 2012 ISSN: 2229-791X www.researchinbiotechnology.com Regular Article Isolation and identification of a new Bacillus strain for amylase production Yasser Bakri*1‚ Hassan Ammouneh1‚ Samir El-Khouri1‚ Muhanad Harba1 and Philippe Thonart2 1Department of Molecular Biology and Biotechnology‚ AECS‚ Damascus‚ Syria‚ P.O.Box 6091 Wallon de Biologie Industrielle‚ Unité de Bio-industrie‚ Faculté de AgroBiotechnologie de Gembloux‚ Université de Liège
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to determine the fundamental effects that temperature has on the growth and survival of bacteria. During the first experiment five different bacterial broth cultures of Escherichia coli‚ Pseudomonas fluorescens‚ Enterococcus faecalis‚ Bacillus subtilis and Bacillus stearothermophilus were individually incubated at temperatures of 5‚ 25‚ 37‚ 45 and 55°C for one week in an aim to distinguish the effect temperature has on growth and survival of the five different species. After one week they were observed
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of the four species. By conducting this methodology‚ the range of acceptable pH limits‚ including the optimum pH for each organism‚ can be determined. The range of pH over which an organism grows is defined by three cardinal points: the minimum pH‚ below which the organism cannot grow‚ the maximum pH‚ above which the organism cannot grow‚ and the optimum pH‚ at which the organism grows best. Escherichia coli are one of several types of bacteria that normally inhabit the intestine of humans. It is
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UNIVERSITY OF DAR –ES-SALAAM COLLEGE OF NATURAL AND APPLIED SCIENCES DEPARTMENT OF MOLECULAR BIOLOGY AND BIOTECHNOLOGY MC206: FOOD MICROBIOLOGY PRACTICALS PRACTICAL 1 MICROORGANISMS IN THE ENVIRONMENT GROUP #:1 NAME: DUSENGEMUNGU Léonce REG #: 2011-04-07086 COURSE INSTRUCTOR: Dr Mugassa S.T Rubindamayugi
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which facilitates differentiation of various elements in a specimen is a general term that can refer to a number of specific processes. Using multiple stains can better differentiate between different microorganisms or cellular components of a single organism. Gram’s Stain is a widely used method of staining bacteria as an aid to their identification. It was originally devised by Hans Christian Joachim Gram‚ a Danish physician. Gram’s Stain differentiates between two major cell wall types. Bacterial species
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PDA plate. Organism Result + or - Appearance/Observations Bacillus cereus Escherichia coli B. Gelatin Hydrolysis (gelatinase production) Note: whether the gelatin deep remains solid or has been liquefied Organism Result + or - Appearance/Observations Bacillus subtilis Escherichia coli C. Coagualse: identification of Staphylococcus aureus Note: whether the rabbit plasma has remained liquid or become solid due to clot formation Organism Result +
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colonies from a mixed population of bacteria. The instructor will provide TSA plates (Tryptic Soy Broth mixed with 15% agar‚ heated to sterilize media and melt agar then poured into a Petri dish) and a culture containing Escherichia coli and/or Bacillus subtilis and/or Staphylococcus aureus and/or Serratia marcescens. Isolation of bacteria using the streak plate technique: Label the bottom of a Petri dish (side containing the agar) (Why?) with your initials‚ date and lab section. Your instructor
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