The first test conducted on unknown bacteria 32 was the Gram stain. From this stain‚ unknown 32 was found to be a Gram-positive cocci. This test eliminated all possible Gram-negative bacteria‚ Gram-positive rods and Gram-positive spirillium. Next‚ the endospore test determined whether or not the Gram-positive bacteria contained endospores. With the use of malachite green‚ steam‚ and safranin it was found that unknown bacteria 32 did not contain endospores. This eliminated Gram-positive cocci Sporosarcina
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MBK Lab 01 – Lab Report Name: ____________________ Section: ___________________ EXPERIMENT 1 TITLE: Observing Bacteria and Blood OBJECTIVE: To gain functional knowledge of microscope operations through practical applications of a microscope in the observation of bacteria and blood. PROCEDURES: Using the microscope‚ an oil immersion lens and observing Bacteria Cultures in Yogurt . Preparing a Blood Slide and observing Blood: After reviewing the section of the manual
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Abstract The primary focus of this lab was on microscopy and simple stains. Crystal violet and Carbol fuchsine‚ simple staining components‚ were used to stain the slide in order to see the different microbes in order to determine their cellular shape and identify unknown ones by comparing. Introduction Bacterial cells are usually colorless because cytoplasm‚ for the most part‚ is transparent. Since the bacteria are colorless‚ it is almost essential to add a stain to make the bacteria more visible
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Margaret E Gibson July 20‚ 2009 Microbiology Dr. Metera Lab Report 3: Labs 7 and 8- Metabolism and Biochemical Tests Abstract This experiment focused on metabolism and biochemical tests. The goal of performing these tests was to differentiate microbes from one another and to compare how metabolic and biochemical processes differ from species to species. The tests performed include: the Fermentation of Sugars Test (sucrose‚ glucose‚ and lactose)‚ the Urease Test‚ the Fermentation
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Grams staining method and reliability of this method. According to me‚ Grams staining process is a simple technique that assists in recognition of etiological agent and therefore can be called as one of the most significant staining practices in microbiology. Though‚ Gram is concerned that the staining process developed by him is imperfect because not all bacteria can be stained by it‚ but in my opinion‚ when we pour ethanol over the slide to wash away the dye then only definite bacteria can retain
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Lab Report #1: Observing Bacteria Microbiology Abstract: This lab exercise familiarized the student with the use of a microscope by observing and identifying various different slides under the microscope. The student practiced observing the given slides under the 10x‚ 40x‚ and 100x (oil immersion) objective lenses‚ which allowed for the identification of the different organism’s shapes and sizes. Purpose: The aim of this exercise is to equip the student with the knowledge
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References: Koneman‚ E. W. (1997). Koneman ’s Color Atlas and Textbook of Diagnostic Microbiology. USA: Lippincott Williams & Wilkins. Madigan‚ et. al (2014). Brock Biology of Microorganisms (14th ed.‚ p. 78). Boston: Pearson. Mahon‚ C.‚ Lehman‚ D.‚ & Manuselis‚ G. (2011). Textbook of Diagnostic Microbiology. Missouri: Elsevier. Tille‚ P. M. (2014). Bailey & Scott ’s Diagnostic Microbiology. Missouri: Elsevier.
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Identifying an Unknown Aldehyde or Ketone Introduction The purpose of this lab is to identify an unknown aldehyde or ketone substance using chemical tests. The chemical tests used in this experiment are solubility‚ Schiff‚ Bisulfite‚ and Iodoform tests. Also‚ a 2‚4-dinitrophenylhydrazone derivative synthesis reaction will be completed from which a melting point will be obtained. The chemical test results and the melting point analysis will be compared to the table of compounds given to find the
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It Smells Like Proteus vulgaris Microbiology Lab Report for Unknown Robert Bhowanidin MCB 2010L / Section 1290 October 24th‚ 2013 The following report will describe both my journey to find my unknown as well as the results that led to my discovery. Before I start‚ I will say that I am 100% positive that my unknown (which was #31) is none other than Proteus vulgaris. My data and the ensuing results from them simply cannot be disputed. From my first batch of results‚ Proteus vulgaris reared
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performed this test‚ the initially slant for unknown microorganism #17 is important. For this lab‚ two identical slants are used for two reasons. Firstly‚ the slant can be used to make sure that there is no contamination from the Nutrient Agar plate. Secondly‚ the second slant will become a stock culture to prevent the shortage of slants during performing the series of tests. Kliger’s Iron Agar tests can be used to determine multiple characteristics of unknown microorganism #17. Kliger’s Iron Agar slants
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