Gram Negative Unknown Lab Report Abstract The Unknown Gram Negative bacterium inoculated in a Tryptic Soy broth medium was randomly selected from a group of other unknowns. In order to identify this unknown the seven different types of biochemical tests will be conducted on this unknown bacterium to identify it out of 6 possible bacteria; Escherichia coli(E. coli)‚ Enterobacter aerogenes (E.aerogenes)‚ Klebsiella pneumoniae (K.pneumoniae)‚ Proteus mirabilis(P.miranilis)‚ Pseudomonas aeruginosa(P
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Unknown Bacterium #22 Purpose The purpose of this experiment was to find the identity two unknown bacteria in the given test tube. Materials •Bacterial Loop •Bunsen Burner • Petri Dish with agar •Crystal Violet •Gram’s Iodine •95% Ethanol •Safrin •Glass Slides •Microscope •Unknown bacteria in test tube Procedure On the first day‚ one plate was streaked qualitatively and left it in the
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our skills we have learned in the lab to identify two unknown organisms. PROCEDURE I was given unknown #76. I performed the streak plating method with my unknown organisms. To perform this exercise I needed a TSA plate and labeled the bottom of it with my name‚ group number and organism. I also divided the bottom into three sections. After that‚ I sterilized my inoculating loop using the bunsen burner flame. After letting the loop cool‚ I reached into my unknown test tube with my loop and grabbed
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Sp2012 (Set all of your margins to ½”) Purpose: The purpose of this lab is to help you become a little familiar with some of the tests that can be typically performed in a clinical or research lab facility. These tests may help in determining a particular pathogen’s growth needs. There are several sections to this lab. Find each section and complete the “Preparing for Class” sections. Preparing for class - Day 1 Read in your lab manual about the following agar mediums: Blood Agar (pg 168)‚ EMB
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Unit 6 Lab Report 1. Objective: To find the percent composition of water in an unknown hydrate. 2. Materials: Goggles‚ apron‚ evaporating dish‚ hot plate‚ and stirring rod 3. Procedure: get the mass of a evaporating dish‚ put a sample of the hydrate onto the evaporating dish and mass the dish with hydrate. Put the dish with hydrate on the hot plate on high heat. Break up any clumps that form during the heating process. Observe any color
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| Gram Negative Unknown | Biology 3444-006 | | Lena Wallace | 11/7/2011 | | Abstract: The purpose of this lab was to identify an unknown bacteria culture using differential tests. The identification of the unknown culture was accomplished by identifying the bacteria based on its specific metabolic characteristics and morphology. It is suggested that culture 11 is a sample of Enterobacter aerogenes. Introduction: This experiment was centered on metabolic and biochemical testing
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first test conducted on unknown bacteria 32 was the Gram stain. From this stain‚ unknown 32 was found to be a Gram-positive cocci. This test eliminated all possible Gram-negative bacteria‚ Gram-positive rods and Gram-positive spirillium. Next‚ the endospore test determined whether or not the Gram-positive bacteria contained endospores. With the use of malachite green‚ steam‚ and safranin it was found that unknown bacteria 32 did not contain endospores. This eliminated Gram-positive cocci Sporosarcina
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1. Title: Double unknown identification of a mixed culture of Gram-negative and Gram-positive bacteria 2. Author: Nick Fiore‚ University of Kansas‚ Biology 402‚ Fall 2014 3:00pm room 6040 3. Abstract: The purpose of this experiment was to isolate two unknown bacteria and perform a series of selective and differential tests to correctly identify each. After the bacteria was isolated a series of differential and selective tests following the dichotomous key attached were used to identify each bacteria
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Escherichia coli Microbiology Lab NAS 185-14L Introduction In this lab experiment we did several test to determine what our unknown bacteria was. To determine this we recorded the results of how the bacteria reacted to different media. Depending on the results of each test we could narrow down the different bacteria to determine what our unknown is. This experiment will also determine if our bacteria is a fermenter of sugars and if it is catalase positive. If the bacteria is a fermenter
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difference between a gram positive bacteria and gram negative bacteria is the differences in cell wall composition. Prokaryotes known as eubacteria have three basic forms: rods‚ cocci and spiral. The bacterial cell wall is the single most important contributor to cell shape. In addition to shape of cell wall‚ presence or absence of flagellum‚ and if present‚ positions of flagellum‚ the eubacteria can be classified according to Gram Stain. First and foremost‚ gram positive bacteria are by
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