by aseptically inoculating a tube of phenol red sucrose broth‚ and a tube of phenol red arabinose broth with the unknown culture and incubating at 37 degrees Celsius for 48 hours. After incubation‚ the two tubes were examined for color change. The eighth test was to find out if the bacteria in question had flagella. The motility test was performed by aseptically inoculating the unknown bacteria into a tube of TSA broth and allowing it to incubate for 24 hours at 37 degrees Celsius. After the TSA
Premium Bacteria Microbiology Microorganism
or clusters and form distinct colonies. Bacteria are classified on the basis of many characteristics. Morphological and physiological features such as cell shape‚ motility‚ formation of spores and other distinguishable structures‚ and reaction to Gram stain is a good start in identifying bacteria. Other staining techniques such as Acid Fast stain are also useful in determining species. More important in identification of a genus and species of bacteria are biological tests‚ including the determination
Premium Staining Microbiology Gram staining
environment and diseases caused by them‚ and how treat it. MATERIAL AND METHODS: An unknown bacterium was handed out
Premium Bacteria Microbiology Microorganism
Gram Stain: The important part of this experiment is being able to determine a bacterium based on its cell wall structure. It also helps indentify if the unknown organism is a Gram positive or Gram negative. This is the initial step that must be taken before any other lab procedure may continue on to ensure the purity is present‚ the arrangement of the cells‚ and the shape the cell has. The staining of the cell starts off with using the primary stain‚ Crystal Violet which is a purple color‚ to begin
Premium
Gram Staining Introduction Prokaryotes are a large group of organisms with no membrane bound organelles. They consist of two domains: Archaea and Bacteria. These organisms are only found in extreme environments such as volcanoes. Prokaryotes are still being researched and are a very diverse group. In this lab we focused on trying to identify if the bacteria found had a lot of peptidoglycan by gram staining. Testing this could be done by using a Petri dish full of agar and testing different bacteria
Premium Bacteria Microbiology Gram staining
Introduction:The objective throughout the gram staining experiment was to classify bacteria through their chemical nature as well as the physical structure of their cell walls. Through this experiment one would be able to observe either a thick or thin layer of peptidoglycan by the appearance of the bacteria staining either gram-positive blue-purple or gram-negative pink. Question: Is there a subsequent effect of the bacteria when decolorizing with alcohol? Hypothesis: If there is either a removal
Premium Bacteria Microbiology Chemistry
In a brief essay‚ identify at least two of the implications implicit in the society reflected in the poem. Support your statements by specific references to the poem. The Unknown Citizen Comment [ANn1]: OK‚ so I need to IDENTIFY two implications implicit in the society. I need to make sure I’m speaking only to the society reflected IN the poem. I’ll make sure to quote the poem itself. by W. H. Auden (To JS/07 M 378 This Marble Monument Is Erected by the State) He was found by the
Premium W. H. Auden The Unknown Citizen Happiness
Service - Our Responsibility Water and Sewerage Authority (WASA) was established by an Act of Parliament in 1965 to manage the water and sewerage sector of Trinidad and Tobago. An essential component of this mandate is the delivery of a safe‚ reliable and efficient water supply to satisfy the demand of all sectors of the economy. Whilst over the ensuing years there has been a steady increase in demand‚ there has not been the commensurate investment in infrastructure and the identification of alternative
Premium Orthography Phoneme
NAME: PRAJWAL BHARADWAJ Ampicillin‚ Vancomycin‚ and Tetracycline Effectiveness on Gram Positive and Gram Negative Bacteria. INTRODUCTION: Bacteria are microbial organisms which are present in various environments. Many bacteria are good and help humans synthesize materials and assist in biological processes‚ such as digestion. However‚ some bacteria can cause harmful diseases. When harmful bacteria infects the body‚ people take antibiotics in order to suppress bacteria. Different antibiotics have
Premium Bacteria Microbiology Antibiotic resistance
on human samples collected from different body sites. The tests are used to detect and identify any microorganisms capable of causing disease. Knowing of unknown microorganism is important on how this microorganism works and how it is structured‚ means knowing how it can affect humans. The purpose of this study was to identify an unknown bacterium by applying all methods that were previously conducted and learned in the medical microbiology laboratory class. **Materials
Premium Staphylococcus Bacteria Staphylococcus aureus