Cesar Bizarroque Wesam Daker Bio 1107k-11 10/25/14 How pH levels impact tyrosinase activity Abstract: The purpose of our research utilizing the different pH levels was to test how a specific pH level would impact tyrosinase activity. First we added 4.0 mL of pH in each corresponding test tube and then added 0.5 mL of substrate (catechol) into each test tube. In the instructions it says to apply your 0.5 mL of tyrosinase (potato extract) as well but you have to blank the spectrophotometer before
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The enzyme tyrosinase was successively extracted by combining a homogenate of a potato and sodium sulfate with ammonium sulfate. Tyrosinase was successfully extracted by taking advantage of solubility properties of certain proteins. A standard curve was generated indicating dopachrome absorbance values through the use of a spectrophotometer and a computer graphing program. A spectrophotometer was used to measure either the amount of light that passed through a solution (transmittance) of the amount
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Introduction: The purpose of this lab was to measure the extent of enzyme reaction on given substrates by means of color change. The reaction followed is given below: Tyrosinase³ Enzyme Pyrocatechol Hydroxyquinone Oxidation/Reduction Pink ³ Brown E+S + [ES] = E+P Enzyme Reaction Hypothesis: If there is an increase in enzyme concentration‚ an increase in reaction temperature‚ or an increase in buffer pH‚ then greater
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level‚ duly recognized both by the Commission and Higher Education (CHED) and TESDA. Back in 2010 our institution enrollment succeeded to a percent of one hundred and fifty. Our computer facilities intends only into a very limited units so that our Lab supervisor easily maintain and create schedules for students who has a computer subjects. By this time‚ Speed Computer College again increased by staggering a percent of two hundred enrollees. One of the main problems of Speed Computer College
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Enzymatic Assay of TYROSINASE (EC 1.14.18.1) PRINCIPLE: L-Tyrosine + O2 L-DOPA Tyrosinase Tyrosinase > L-DOPA > L-DOPA-quinone + H20 Abbreviation used: L-DOPA = L-3‚4-Dihydroxyphenylalanine CONDITIONS: METHOD: REAGENTS: A. 50 mM Potassium Phosphate Buffer‚ pH 6.5 at 25° C (Prepare 50 ml in deionized water using Potassium Phosphate‚ Monobasic‚ Anhydrous‚ Sigma Prod. No. P5379. Adjust to pH 6.5 at 25° C with 1 M KOH.) 1 mM L-Tyrosine Solution (Prepare 100 ml in deionized water using L-Tyrosine
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Computer Lab Rules & Regulations Electronic workstations may only be used by current Swinburne University students and staffs. Swinburne identity card must be presented on request. Swinburne computing facilities should only be used for educational‚ research and administrative purposes of Swinburne. All other uses are strictly prohibited. The following rules and terms apply to all computers on campus. Terms and Conditions 1. All users must abide by the license requirements of any software
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NATIONAL UNIVERSITY OF RWANDA FACULTY OF APPLIED SCIENCES DEPARTMENT OF COMPUTER SCIENCE BACC IV ACADEMIC YEAR: 2012-2013 CHAPTER 1. TOPIC: DESIGN AND IMPLEMENTATION OF AN ONLINE COMPUTER LABS MONITORING SYSTEM CASE STUDY: NATIONAL UNIVERSITY OF RWANDA ICT CENTER/Helpdesk Unit
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Case Analysis Kaspersky Lab: From Russia with Anti-virus Industry Background: Software Security Cybercrime has become a fast growing concern for the 21st century as businesses‚ institutions and individuals grow into an interconnected web of computer networks. Online business transactions‚ along with the sharing of personal information‚ are vulnerable to a host of disasters that can reap economic and social havoc. Some sources say that today‚ cybercrime costs more than $1.0 trillion to society--Global
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mushroom tyrosinase and the inhibiting effects of thiourea‚ cinnamic acid and benzoic acid BIOL/BIOC 393 L03 Dr. Judit Moldovan Submitted: Nov. 22nd‚ 2010 By: Jackie Minnick (Partners Amanda Verwoerd & Kersti Ojamaa) Study of stereospecificity in mushroom tyrosinase and the inhibiting effects of thiourea‚ cinnamic acid and benzoic acid. Jackie Minnick This paper reports experiments on the stereospecificity observed in the monophenolase and diphenolase activities of mushroom tyrosinase and the
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the science lab. In science‚ we would participate in a lab. One of my favorite labs was the owl pellet dissection. Owl pellets are owl throw up. In owl pellets you can find the bones of animals the owl consumed. In this lab we were to take out the bones we found in the pellet and construct the animal we found inside. Most people‚ like me‚ had a sort of rodent‚ but some people found birds in their pellet. Another one of my favorite things we did in science was the Sharpie tie dye lab in fifth grade
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