Proteins are polymeric chains that are built from monomers called amino acids. All structural and functional properties of proteins derive from the chemical properties of the polypeptide chain. There are four levels of protein structural organization: primary‚ secondary‚ tertiary‚ and quaternary. Primary structure is defined as the linear sequence of amino acids in a polypeptide chain. The secondary structure refers to certain regular geometric figures of the chain. Tertiary structure results from
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Lab 2: Internet Resources for Protein Biochemistry Part 1-1: A) Protein Accession Number = P00939 Name of Protein: Full Name = Triosephosphate isomerase; Short Name=TIM Organism where the protein is found: Oryctolagus cuniculus = Rabbit Nuber of amino acids in the protein: AA = 248 B) * Amino Acid Sequence: >gi|136066|sp|P00939.1|TPIS_RABIT RecName: Full=Triosephosphate isomerase; Short=TIM;
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DENATURATION OF PROTEINS Abstract The experiment was done to be able to understand how various denaturants such as HCl and NaOH affects proteins. It was observed that different denaturants act upon or denature protein differently. This was determined using the principle of viscometry. An Ostwald viscometer was used to measure the viscosity of the prepared native‚ blank‚ denatured native and blank with denaturant solutions. The time required for the said solutions to pass through the viscometer
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Identifying sites of protein synthesis in Chlamydomonas using erythromycin and cyclohexamide as protein synthesis inhibitors. October 16‚ 2009 Introduction: In living cells‚ prokaryotic or eukaryotic‚ the synthesis (construction) of proteins is accomplished by similar machinery. Amino acids‚ ribosomes‚ messenger RNA (mRNA)‚ and transfer RNA (tRNA)‚ are all necessary for the building of functional proteins in a cell. Ribosomes are the site of protein synthesis in a cell‚ and there are two
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Abstract This experiment aimed to study the effect of various denaturants on albumin and casein protein extracts through viscosity measurements. 5 mL samples of native and denatured protein solutions were prepared‚ using -mercaptoethanol‚ urea and SDS as denaturants for albumin‚ and NaOH‚ NaCL‚ HCL‚ -mercaptoethanol‚ urea and SDS for casein. 5 mL blank solutions for each denaturant used were also prepared. The viscosity of the solutions were determined using Ostwald viscometer. ________
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TITLE: The Amount of Protein in Chicken Tissues over Cooked Various Periods of Time. ABSTRACT: In this lab‚ we are using a BioRad protein assay dye to determine the concentration of protein in our chicken. The dye binds to the amino acid residues‚ which allow us to find the concentration of protein (BioRad Protein Assay for Tissues). Our hypothesis was the longer chicken is cooked the less protein is available. To test our hypothesis‚ we made samples using our chicken and distilled water to determine
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Exercise 1: Cell Transport Mechanisms and Permeability: Activity 1: Simulating Dialysis (Simple Diffusion) Lab Report Pre-lab Quiz Results You have not completed the Pre-lab Quiz. 09/20/11 page 1 Experiment Results Predict Question: Predict Question 1: The molecular weight of urea is 60.07. Do you think urea will diffuse through the 20 MWCO membrane? Your answer : c. No‚ not at all. Predict Question 2: Recall that glucose is a monosaccharide‚ albumin is a protein with 607 amino acids‚ and
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Transport Systems Essay In single celled organisms‚ nutrients‚ water and various substances that are need for basic cellular processes can easily be transported in and out through the cell membrane. This is an effective transport system for microscopic organisms‚ but multicellular organisms require a more complex transport system to sustain itself. Take humans for example; unlike a cell‚ only the outer layer of skin is in contact with the body’s outside environment. It’s not possible for substances
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accepted the origin of all life on Earth. In the article‚ “The Proof is in the Proteins: Tests Supports Universal Common Ancestor for All Life”‚ by Katherine Harmon‚ a scientist by the name of Douglas Theobold carried out statistical analysis on 23 preserved proteins that were preserved over three major domains of life. The result of these analyses supported Darwin’s evolutionary theory‚ by demonstrating that the protein sequences of life today came from a common universal ancestor rather than separate
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Protein Essay- Erin Keegan What are proteins? A polypeptide is a chain of monomers joined up to make a polymer. Proteins are organic polypeptides made up of a chain of amino acid residues- they’re residues because they lose a water molecule. The amino acids (monomers) join up by forming peptide bonds. When two amino acids join together‚ they become a dipeptide. This happens when the carboxyl group of an amino acid and the amino group of another amino acid link up through a condensation reaction (which
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