BABS1201 Group Enzymes Project Group Protocol Including Equipment and Reagents List Lab Day: Wednesday Lab Time: 10am – 1pm Are you in Lab G20 (furthest from BSB Student Office) or Lab G21 (closest to BSB Student Office)?: Demonstrator Name: Daniel Winters Names of Group Members: Johnny Nguyen‚ Therese Pham‚ Linda Tang Name of Enzyme You are Investigating: Amylase Brief Background: Amylase is a digestive enzyme‚ produced mainly by the salivary glands and the pancreas‚ to break down
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STEP BY WICKED STEP Themes ~ Step By Wicked Step Family relationships Coping with new family members for example Pixie understands that her new stepmother is having as much trouble ‘adjusting’ as she is. Family unity The nucleus of moral values‚ “the family unit‚” is being invaded by outsiders (‘step’ or ‘steps’) and this causes disharmony. Dealing with adversities Young children and adults may find difficulties dealing with frustrations‚ fears and sadness. Being happy ‘Life has
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Rate of Reaction that Enzyme Concentration‚ pH‚ and Temperatures Have on the Amylase Enzymes Color Disappearance Abstract: Compare reaction rates of the concentrations‚ pH’s‚ and temperatures of the enzyme Amylase. At what concentrates do the substrate molecules collide with each other‚ making the reaction possible? At what pH levels do the 3D molecular structures change breaking the H-bond and/or denaturize? At what temperatures do the collisions of the substrate molecules happen? Proteins
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Discovery Restriction enzymes were discovered 40 years ago during investigations into the phenomenon of host-specific restriction and modification of bacterial viruses. Restriction enzymes protect bacteria from infections by viruses‚ and it is generally accepted that this is their role in nature. They function as microbial immune systems. When a strain of E. coli lacking a restriction enzyme is infected with a virus‚ most virus particles can initiate a successful infection. When the same strain
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The Effect Of Enzyme Concentration On Enzyme Activity The pancreatic duct in individuals who have cystic fibrosis frequently becomes blocked‚ reducing or preventing the release of pancreatic enzymes into the small intestine. The aim of this activity is to investigate the effect of a reduction in enzyme concentration on the rate of reaction‚ in this case the breakdown of protein by protease enzymes. Aim – Milk powder contains a white protein called casein. A white suspension of
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Enzyme activity The introduction is a statement of the subject and objectives of the experiment and presents your hypothesis. Relevant background information (appropriately referenced) is given in sufficient detail that a person unfamiliar with the topic can understand the nature of the experiment. A good introduction is clear and concise. Carbohydrates and proteins should be explained in terms of their structure and major functions in cells. Your intro should end with a brief overview of the amylase
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on the panel above to save your report) Activity: Name: Instructor: Date: Enzyme Activity Pam Campbell Id 0002337 Dr. Murphy Nmezi August 9‚ 2011 Predictions 1. Sucrase will have the greatest activity at pH 6 2. Sucrase will have the greatest activity at 40 °C (104 °F) 3. Sucrase activity increases with increasing sucrose concentration until a plateau is reached. Materials and Methods Effect of pH on Enzyme Activity. 1. Dependent Variable. amount of product (glucose and fructose) produced
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In this theory‚ Lewin described the change process in three phases‚ unfreezing‚ movement and refreezing. During the first step‚ the existing situation was routine and boring. At this stage motivation to bring the change was used in order to achieve unfreezing. The second step in Lewin’s three step change theory is movement. In this stage‚ the action was taken which is persuading the nurse educator to implement the change. The final step is refreezing‚ which means that the action has been implemented
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BIOCHEMISTRY 304 Enzyme Kinetic Sample Problems #1 September 2004 1 Given the reaction k1 kp E + S ES E + P k-1 where k1 = 1 x 107 M-1 sec-1 k-1 = 1 x 102 sec-1‚ and kp = 3 x 102 sec-1 a) Calculate Ks b) Calculate Km (a) k-1 1 x 102 sec-1 Ks = k1 = 1 x 107 M-1 sec-1 = 1 x 10-5 M (b) k-1 + kp (1 x 102 sec-1) + (3 x 102 sec-1)
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The bonding of an enzyme to its substrate forms an enzyme-substrate complex. The catalytic action of the enzyme converts its substrate into the product or products of the reaction. Each reaction is extremely specific‚ distinguishing between closely related compounds‚ including isomers. For example‚ the enzyme sucrase will only act on sucrose and will not bind to any other disaccharide. The molecular recognition of enzymes is due to the fact that they are proteins‚ which are defined as being macromolecules
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