Temperature effects on the growth of microorganisms Introduction: The purpose of this experiment was to determine the effects that temperature has on three different organisms. Temperature is one of the most important environmental factors affecting growth and survival of microorganisms². The three organisms used where Escherichia coli‚ Pseudomonas fluorescens‚ and Bacillus stearothermophilis. Most bacteria grow within a particular temperature range. The minimum growth temperature is
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Investigating Effects of duration of heating on permeability of beetroot cell membrane Introduction: Cell membranes contain many different types of molecules which have different roles in the overall structure of the membrane. Phospholipids form a bilayer‚ which is the basic structure of the membrane. Their non-polar tails form a barrier to most water soluble substances. Membrane proteins serves as channels for transport of metabolites‚ some act as enzymes or carriers‚ while some are receptors
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Membrane Behavior Lab Abstract: Introduction: The permeability of a cell to solutes in an aqueous medium depends upon the physical and chemical make–up of the membrane. The maintenance of the living cell depends upon the continued presence and functioning of a selectively permeable membrane. If the nature of the membrane is changed or altered in any way‚ this may well affect its permeability and thus the properties of the cell of which it is a part. Irreversible changes in the permeability
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Lab Report Effect of temperature on enzyme activity I. Purpose: Determine how effect of temperature on enzyme (catalyse) activity (in the liver). II. Materials * Raw liver * Forceps * 50mL 1% hydrogen Peroxide (H * 25mL graduated cylinder * 50mL‚ 400mL beakers * Pureed liver * 5 Filter-paper disks * Paper towels * Timer or stopwatch * Thermometer * Hot plate III. Procedure 1.Gather all necessary materials; start
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apron or lab coat to protect our eyes and clothes. As we are using enzymes and Hydrogen Peroxide we need to be extra careful‚ ensuring they don’t come into contact with our eyes‚ skin or clothes. Catalyse is an enzyme found in all living cells. It makes Hydrogen Peroxide decompose into water and Oxygen. We will be measuring the amount of Oxygen released from the Hydrogen Peroxide. In order to do this we will use a measuring cylinder. This piece of apparatus measures the amount of
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test for the effects of temperature on catalase activity. Introduction: Enzymes are proteins that act as catalysts and help complex reactions to occur everywhere in life. Enzymes affect a chemical reaction by acting as a catalyst‚ which is to say‚ it speeds up the reaction. However‚ extreme ph and temperature hinders enzyme function. Optimal activity of an enzyme is the optimal environment that an enzyme requires to function‚ which means having the optimum ph and temperature to catalyze a
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Effect of temperature on enzyme activity In this experiment… Independent variable: Temperature of the amylase Dependent variable: Enzyme activity which is measured by the time for disappearance of starch Controlled variables: Volume of amylase; volume of starch solution; concentration of amylase; concentration of starch solution Prediction of results i) At low temperature‚ the rate of amylase activity is very low. ii) At optimum (=best) temperature‚ the rate of amylase activity is the
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Effect of Temperature on Solubility Lab Purpose: What is the solubility of minerals in water? What is the relationship between temperature and solubility? Hypothesis: If salt and sugar are each tested in water of varying temperatures‚ then salt and sugar’s solubility will increase as the temperature also increases. Materials: Two 250 mL beakers Tap water 100 mL graduated cylinder Hot plate Two petri dishes Glass stirring rod Salt Sugar Thermometer
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Introduction For this experiment‚ we are going to determine the effect of temperature on solubility‚ to be done in a chemical by dissolving a solute in a definite amount of solution which is saturated. Specifically‚ the goal of this experiment is to prepare a saturated solution of Na2C2O4 in water at different temperatures‚ determine the effect of temperature in solubility‚ and to apply Le Chatelier’s Principle. We can do all this by simply titrating a certain amount of standard KMnO4‚ and measuring
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Experiment 4 – Effect of Temperature on Enzyme Activity Aim To study the effects of temperature on the activity of amylase enzyme on starch solution. Introduction Enzymes are widely known as biological catalyst. Almost all cellular reactions are controlled and guarded by enzymes. Virtually every metabolic reaction which takes place within a living organisms are catalyzed by enzymes. Enzymes are complex three-dimensional globular proteins. Some of the enzymes are built up off proteins and
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