reactions in cells. The specific function of the enzyme in this experiment was to see if it affected the activity if it was exposed to different temperatures. A substrate is a material or substance which the enzyme acts. The substrate in our lab was hydrogen peroxide. Procedure For our experiment we used‚ three test tubes‚ nine milliliters of potato
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Osmosis is a passive movement of water through a selectively permeable membrane moves from an area of higher concentration to an area of lower concentration. In this lab‚ we can determine the direction of diffusion of small molecules by measuring the diffusion of small molecules through dialysis tubing. This tubing acts as a selectively permeable membrane‚ and allow to pass larger molecules slowly. Introduction: Cells have kinetic energy and it causes the molecules of the cell to move around
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pH & Enzyme Action Aim: To inspect the effects of the pH on enzymes. Apparatus: 100 cm³ Beaker 3 – 5cm³ Syringes 2 Test Tube Racks with 8 Test Tubes Stop-watch Ruler Dropping bottle of detergent Marker Pen Masking Tape 400cm³ Hydrogen Peroxide 200cm³ Liver Catalase Solution 100cm³ of following Buffer Solution – pH5 pH7 pH9 pH11 Method: The materials were collected. The test tube rack one with 4 test tubes had been labelled A to D. The 2cm³ of each buffer solution
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determine concentration. Molarity is found by dividing the number of moles of a solute by the volume of the solution in liters. Multiple series of solutions with different concentrations can be used by diluting the concentration. The dilution technique is: Number Moles Concentrated Solution = Number Moles Dilute Solution. An instrument called a spectrophotometer detects the amount of light that passes through the sample and the percent transmittance can be recorded from the meter. In the lab‚ multiple
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University of Texas at Tyler Lab 3C: Purification of L-Lactate Dehydrogenase By Affinity Chromatography on Cibacron-Blue Sepharose David Alexander 10-15-2014 Dr. Black Chem 4135.001 Abstract: Like the previous experiments‚ the ultimate goal of this lab was to purify the enzyme sample. However‚ this is the last lab for purification and high level techniques of purification were employed to achieve this. Dialysis was used first‚ lowering the small-molecule concentration within the sample. Finally
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DNA denaturation is a process that breaks apart the double stranded helical structure typical of DNA into two single DNA strands. This occurs by breaking the hydrogen bonds that hold the base pairs together in a double helix. A hindrance to DNA denaturation is base stacking; base stacking energies are so strong that they essentially cause adjacent bases to cohere. Since this step was not performed in the laboratory
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Osmosis Rates in Artificial Cells Daniel George Department of Biology Grand Valley State University 1 Campus Drive Allendale‚ MI 49401 georged@mail.gvsu.edu Abstract The lab for this paper was conducted for the topic of osmosis‚ the movement of water from high to low concentration. Five artificial cells were created‚ each being filled with different concentrated solutions of sucrose. These artificial cells were placed in hypertonic‚ hypotonic‚ or isotonic solutions for a period of 90
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Biology 160 Lab Manual Tacoma Community College Last update 06/03/2013 This page left intentionally blank Table of Contents Table of Contents i Laboratory 1: Principles of the Scientific Method 4 In-Lab Report Sheet 12 This page has been left intentionally blank 27 Laboratory 2: Scientific Methodology & Enzyme Activity 28 In-Lab Report Sheet 28 Objectives 33 Introduction 33 Parts of the Swift M5 Microscope 33
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followed. Next‚ for further data‚ MSA results were recorded‚ along with antibiotic observations of demo plates. Lastly‚ with the information gathered‚ it was identified that the unknown sample was Streptococcus salivarius. Introduction: In this lab‚ the exploration of an unknown gram positive bacteria was conducted. What is known about gram positive microorganisms is that their cell walls are composed of a thick peptidoglycan layer‚ which retains the purple color of crystal violet during a Gram
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City University of Hong Kong Dept. of Physics & Materials Science AP2104 Mechanics of Solids Laboratory Manual Experiment 1 Pure Bending of a Beam Experiment 2 Torsional Deformations Experiment 3 Yield Criteria for Ductile Materials under Plane Stresses Experiment 1 Pure Bending of a Beam Objective 1. To examine the stresses at various positions of the beam under a constant load of pure bending. 2. To determine the curvature of deflection of the beam. Introduction
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