specific for one particular reaction or group of related reactions. Many reactions cannot occur without the correct enzyme present. They are often named by adding "ase" to the name of the substrate. Example: Dehydrogenases are enzymes that remove hydrogen. Induced-fit Theory The shape of the enzyme must match the shape of the substrate. Enzymes are therefore very specific; they will only function correctly if the shape of the substrate matches the active site. Enzyme and substrates An enzyme-substrate
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Unknown Lab Report Unknown Organism #6 Ann Le (Phuoc) May 6‚ 2010 Dr. Carrington Microbiology Lab- MW 12:50 Le 1 I. Introduction My unknown organism #6 is Morganella morganii‚ which is a gram-negative bacillus rods commonly found in the environment and also in the intestinal tracts of humans‚ mammals‚ and reptiles as a normal flora. (3‚ 5) This bacterium Morganella morganii‚ was first discovered in the 1906 by a British bacteriologist named H. de R. Morgan. (2) Despite its wide
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HUMAN PHYSIOLOGY REPORT DAPHNIA Aim The aim of this experiment is to investigate the effect of temperature and various substances on the heart beat of Daphnia‚ a water flea. Introduction Daphnia belongs to a group of small‚ freshwater crustaceans of the order Cladocera commonly called water fleas. They are very suitable for laboratory studies due to the nature of their transparent exoskeletons. Hence‚ it is possible to watch Daphnia hearts without dissecting them. Daphnia is ectothermic and
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by the concentration of starch‚ or whether the results I got were due to the amounts of solution used. This would make both my results and conclusion invalid and unreliable. I also made sure that the temperature of the water bath was at the correct temperature and I kept on checking the temperature of both my amylase‚ and starch using a thermometer. I made sure both solutions were at the same temperature at all times throughout my experiments‚ so that temperature would not have an effect on my results
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Osmosis is a passive movement of water through a selectively permeable membrane moves from an area of higher concentration to an area of lower concentration. In this lab‚ we can determine the direction of diffusion of small molecules by measuring the diffusion of small molecules through dialysis tubing. This tubing acts as a selectively permeable membrane‚ and allow to pass larger molecules slowly. Introduction: Cells have kinetic energy and it causes the molecules of the cell to move around
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2011 Lab Partners: Kristen‚ Tania and Betty Introduction When using different methods to measure pH levels there are some tools that can be useful. Some more than others but by putting into action the different methods it may determine which tools will work best and give the best results when testing the pH within a solution. The pH‚ which stands for the proportion of hydrogen ions in a solution
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University of Texas at Tyler Lab 3C: Purification of L-Lactate Dehydrogenase By Affinity Chromatography on Cibacron-Blue Sepharose David Alexander 10-15-2014 Dr. Black Chem 4135.001 Abstract: Like the previous experiments‚ the ultimate goal of this lab was to purify the enzyme sample. However‚ this is the last lab for purification and high level techniques of purification were employed to achieve this. Dialysis was used first‚ lowering the small-molecule concentration within the sample. Finally
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determine concentration. Molarity is found by dividing the number of moles of a solute by the volume of the solution in liters. Multiple series of solutions with different concentrations can be used by diluting the concentration. The dilution technique is: Number Moles Concentrated Solution = Number Moles Dilute Solution. An instrument called a spectrophotometer detects the amount of light that passes through the sample and the percent transmittance can be recorded from the meter. In the lab‚ multiple
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reactions in cells. The specific function of the enzyme in this experiment was to see if it affected the activity if it was exposed to different temperatures. A substrate is a material or substance which the enzyme acts. The substrate in our lab was hydrogen peroxide. Procedure For our experiment we used‚ three test tubes‚ nine milliliters of potato
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DNA denaturation is a process that breaks apart the double stranded helical structure typical of DNA into two single DNA strands. This occurs by breaking the hydrogen bonds that hold the base pairs together in a double helix. A hindrance to DNA denaturation is base stacking; base stacking energies are so strong that they essentially cause adjacent bases to cohere. Since this step was not performed in the laboratory
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