Title Application of DNA Barcodes to Identify Various Plant Species Abstract In this experiment we applied barcodes to plants in order to identify what species they are classified under. We also compared the DNA sequences of different plant species using the ribulose-biphosphate carboxylase gene (rbcL). We took samples from a plant called Chard and performed PCR‚ DNA amplification and quantification and sequenced the DNA. During the experiment‚ we hypothesized that this year’s “nonspinach”
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investigated the amount of DNA extracted from strawberries. This was done by using the independent variable of alcohol to affect the dependant variable of the amount of DNA extracted. This was done to find out if primary or secondary alcohol would create more DNA precipitate than the other. For this the primary alcohols used were; methanol and ethanol‚ and the secondary alcohol was; isopropyl. Of this the secondary alcohol‚ isopropyl was discovered to be the most effect alcohol to make DNA precipitate‚ as it
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DNA Extraction Lab Purpose: To compare the amount of DNA extracted from two different species‚ despite using the same method. Hypothesis: I predict that the liver will produce a higher quantity of DNA than the strawberry. This is because I believe that animals have a higher DNA yield because our structure is more complex than a plant’s structure. Materials: -Sample of Strawberries -Zip lock bag -DNA extraction buffer -Cold ethanol -Glass rod -Double ply cheese cloth -Two test tubes
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Procedure 2: DNA Extraction from Cheek Cells Materials: Water‚ Clear Dish Soap‚ Table Salt‚ Isopropyl Alcohol (70%) or Ethanol‚ Food Coloring 1. To 200 Ml drinking water add two teaspoons of salt 2. Gargle the salt water for 1 minute. 3. Spit the gargled water into a beaker (or new cup). Now your cheek cells are suspended in the salt water. 4. Gently stir the salt water with one drop of soap (try to avoid air bubbles) 5. In a separate beaker (or cup)‚ mix 20 ml isopropyl alcohol and 1-3 drops
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3.1 Source of the Recombinant DNA Polymerase SK72 The recombinant E. coli BL21 (DE3) containing the pET 32b/ DNA polymerase SK72 gene was provided by the Laboratory of Enzyme and Microbial Technology‚ Institute of Bioscience‚ Universiti Putra Malaysia. 3.2 Preparation of the Lysogeny Broth (LB) Agar Plates LB agar plates were prepared by dissolving 10g tryptone‚ 5g yeast extract‚ 5g NaCl and 15g bacterial agar in 950mL of deionized water. The pH of the medium was adjusted to 7.5 using 1M NaOH before
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Wild Strawberries What is the matter with Isak Borg and what can he do to change himself? Wild Strawberries tells the tale of an elderly doctor‚ Isak Borg. He thinks he has lived out a full and prosperous life but in fact he has lived his life somewhat devoid of emotional attachment by choice. He admits that some of his choices has brought him loneliness. The person who is closest to him is the maid who helps take care of him. He finally realizes that his life is not as complete as it should
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1.0 Introduction The report “Business Plan of RedBerries Bangladesh” has been prepared to outline a business plan as a partial requirement for the course Entrepreneurship‚W201 instructed by Mr. Sheikh Morshed Jahan‚ Assistant Professor‚ IBA ‚ University of Dhaka. This report focuses on outlining an entrepreneurship venture into the strawberry cultivation industry of our country. This is a very new industry in our country‚ and while the growth has slowed down in the last years‚ this industry still
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Discusses his lack of authority to write a book‚ but decides to write one anyway. Discusses that he is the one writing the book‚ however his thought are no different than the thoughts of the youth of another generation; what you think at nineteen is universal across all generations‚ youth as a movement has “always been happening.” (Intro) He wants to do something about racism‚ poverty‚ and war. He discusses who they are – kids who have “hopes and fears… ups and downs.” They’re people in NYC who
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LSM1102 Lab Report Introduction Transformation is a process which involves plasmid DNA being bound to the cell surface and the subsequent uptake of DNA by the cell (Panja et al.‚ 2008). For artificial transformation of E. coli cells with plasmids‚ plasmid DNA has to be extracted from bacterial cells using the High-Speed Plasmid Mini Kit‚ which is then mixed with competent E. coli cells followed by heat shock and the streaking of transformed cells on two different types of agar plate (LB and LB+ampicillin)
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Roy Levin Bio 11 Lab Dr.Izquierdo Analysis of Macromolecules in Tissue Homogenates of Bos taurusMaterials and Methods The homogenates provided were made by homogenizing tissues in a sucrose phosphate buffer in a 1:20 ratio. The protein concentration in bovine cells was measured by diluting the homogenate with a 1:5 ratio; 50 microliters of homogenate and 200 microliters of water. Then 5 known protein concentration samples which were 0.4‚ 0.8‚ 1.2‚ 1.6‚ 2.0 mg/ml of bovine serum were used to
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