Experiment 2: Starch Hydrolysis by Amylase Theoretical Background Polymers of carbohydrates are called polysaccharides‚ and make up some of the most important naturally occurring compounds [1]. They have thousands of monosaccharide units linked to each other by oxygen bridges. They include starch‚ glycogen‚ and cellulose‚ all three of which yield only glucose when completely hydrolyzed [2]. A B Figure 1. Starch (amylose) (A) and cellulose (B) Starch
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The Effect of Temperature on Animal and Fungal Amylase’s Ability to Breakdown Starch. Abstract This experiment was designed to test the reaction of the enzyme amylase at various temperatures. There were two different kinds of amylase being tested‚ one was fungal amylase also known as aspergillus oryzae and human amylase. The changes in temperature effect the rate at which an enzyme and a substrate collide. When the temperature is too high the active site changes shape or denatures‚ once this
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Solve the Murder Koreen Clarke October 29‚ 2014 Biology 1 Cellular Processes Lab Section 903 Tianna Clarke Materials and Methods Part I – Restriction Enzyme Digestion To begin this experiment‚ the DNA molecules must be cut into smaller fragments with distinct enzymes called Restriction Enzymes through a process called Restriction Enzyme Digestion. Four microtest tubes were labeled 1 through 4 and added 10 µl of Enzyme Reaction Buffer to each of the four reaction tubes using a micropipette
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LABORATORY REPORT Activity: Effect of Dietary Fiber on Transit Time and Bile Name: Hope Schallert Instructor: Dr. Weithop Date: 03.08.2015 Predictions Dietary fiber will: increase transit time Dietary fiber will increase the amount of bile acid in solution Materials and Methods Effect of Dietary Fiber on Transit Time 1. Dependent variable. number of radiopaque markers in GI tract 2. Independent variable. amount and type of fiber in meal 3. Controlled variables. calories in meal‚ nutrients
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Digestion and Enzymes – APP. Hypothesis: The enzyme‚ Amylase which is used to break down carbohydrates will work the best when heated at 40°C. Also‚ as the temperature increases the reaction rate of amylase increases too. However‚ the reaction rate of amylase will start decreasing when the temperature reaches the enzyme’s optimal temperature. Many enzymes are specific for a certain substrate. For example‚ lipase is a specific enzyme for fat substrates and protease‚ a specific enzyme for
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Defn. of Digestion: the process whereby a biological entity processes a substance‚ in order to chemically convert the substance into nutrients. It usually involves mechanical manipulation and chemical action. Mechanical Digestion What is it? This type of digestion involves the mixing‚ grinding or crushing of large pieces of food into smaller places. Why does it take place? So that the food we consume is broken down into smaller pieces before we swallow it which in turn makes process of
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Digestion/ Absorption and Homeostasis In order to survive‚ our bodies must bring in energy and nutrients for the cells of the body through eating and digestion of food. To be carried to the cells of the body by the blood stream‚ food must be broken down to molecules. This breaking down of food into molecules‚ small enough to be absorbed into and carried through the blood stream‚ is carried out by the digestive system through the process of digestion and absorption. Digestion is the process of
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EXPERIMENT 13 DIGESTION IN THE SMALL INTESTINES ------------------------------------------------- ABSTRACT The small intestine serves as the site of major digestive and absorptive processes. In this experiment‚ the action of pancreatic enzymes on representative samples of each food group under different conditions‚ such as increased/decreased pH and presence of other substances‚ were observed. A pancreatin solution was first prepared from a hog pancreas and was completely neutralized using
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PROCESS OF DIGESTION 1 Process of Digestion Amy Wallace EN 101 February 27‚ 2013 Miller-Motte Technical College PROCESS OF DIGESTION 2 Digestion is an important process needed for maintaining and providing energy to living organisms. Throughout the digestion process‚ food is broken down into small molecules‚ where it can be absorbed easily. There are many organs that aid
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2 Y Nutrition in Animals food into simpler substances is called digestion. You have studied in Class VI that food consists of many components. Try to recall and list them below: 1. ______________________ 2. ______________________ 3. ______________________ 4. ______________________ 5. ______________________ 6. ______________________ no t The components of food such as carbohydrates are complex substances. These complex substances cannot be utilised as such. So they are broken down into
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