Temperature and pH have on the Activity of Porcine Pancreatic Amylase Introduction There are many types of enzymes and each has a specific job. Enzymes are particular types of proteins that help to speed up some reactions‚ such as reactants going to products. One of them is the amylase enzyme. Amylases are found in saliva‚ and pancreatic secretions of the small intestine. The function of amylase is to break down big molecules of starch into small molecules like glucose; this process is called hydrolysis
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Starch From Wikipedia‚ the free encyclopedia Starch Identifiers CAS number 9005-25-8 EC-number 232-679-6 RTECS number GM5090000 Properties Molecular formula variable Molar mass variable Appearance white powder Density 1.5 g/cm3 Melting point decomp. Solubility in water none Hazards MSDS ICSC 1553 EU Index not listed Autoignition temperature 410 °C (verify) (what is: / ?) Except where noted otherwise‚ data are given for materials in their standard state
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Title: Starch Aims 1. To identify starch in food. 2. To study the microscopic appearances of raw and heated starch. 3. To compare the viscosity of various gelatinized starch solutions. Introduction Starch is one of the most abundant substances on the earth. It can be found in seeds‚ grains‚ and roots of many crops where it is synthesized in granular form. Starch granules are packages of starch molecules. The importance of starch is well-known‚ as is its central role in human diet.
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Introduction In the amylase lab‚ it was being tested if amylase‚ an enzyme found in saliva‚ would be denatured by being put in an acid or high temperatures. This lab is about denaturing amylase. It is tested by exposing it to pH and temperature changes. It is then mixed with Benedict’s solution‚ is a solution that changes color from blue to reddish brown when maltose is present. Amylase breaks starch into maltose‚ so is the amylase isn’t denatured‚ it should change colors. Amylase is an enzyme.
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Lab Report DIFFUSION AND THE EFFECT OF AMYLASE. Aim: We will interpret and explain the activity of an enzyme added to a solution. We will analyze reactions in different pH ranges. The starch solution will appear blue when mixed with iodine. But after mixing the enzyme amylase to the starch‚ the starches are broken down into simple sugars. Now‚ when the iodine is added‚ no color change exist. Instruments and materials used: 1.) Dialysis bags x2 2.)
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10 mmol/L | Albumin | 30 g/L | Glucose | 12 mmol/L | Amylase | 5000 U/L --- Normal Range: 60-180 U/L | Serum: Comment: The diagnosis of acute pancreatitis is based on the clinical history‚ evidence of inflammation is known usually by computerized tomography (CT scan) and the finding of a high serum (or sometimes urinary) amylase activity. It is effectively a diagnosis of exclusion: the finding of a very high serum amylase activity is very suggestive but is not on its own diagnostic
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Experiment 9. The action of saliva on starch Study the flow chart on p. 9.02 for a few minutes to gain an idea of the outline of the experiment. (a) Prepare a water bath by using a Bunsen burner to heat some water in a beaker on a tripod and gauze till it boils; then turn the flame down to keep the water just boiling. While waiting for the water to boil‚ carry on from (b). (b) Label eight test-tubes 1 - 8 and in tube 1 collect saliva as follows: (i) Thoroughly rinse the mouth with
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Introduction: In this lab i used glucose and starch to show if the cell membrane is permeable‚ impermeable‚ or selectively permeable to these substances. Dialysis tubing is a plastic like substance that has small pores in it. These pores allow certain chemicals to flow into and out of the cells. The size of the molecule determines what goes through the tubing. On of the materials that we will be using is glucose. glucose is a simple sugar that easily dissolves in water. To test for the presence of
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Affect of pH on Porcine Pancreatic Alpha-Amylase Activity Introduction Proteins function in a variety of different ways‚ and one of their fundamental tasks is to act as enzymes. Enzymes are extremely important in controlling reaction speed (by initiating and regulating biological activity)‚ cell communication‚ and growth. One particularly significant enzyme is amylase‚ which catalyzes the hydrolysis of alpha glycosidic linkages of amylose‚ starch components‚ and other oligosaccharides (Qian
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Dialysis of starch‚ glucose and sucrose Introduction: Research question: Does the dialysis tube only allow certain substances to pass through the pores because of their size? Dependent and Independent variables: Independent variables: The temperature of the classroom The size of the molecules The size of the pores in the tube The concentration of the indicators Dependent variables: The substance will either pass the pores of the tube or not The result will vary in darkness (color) Controlled
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