Ion Exchange Chromatography Week 10 TAG Question 1: Chapter 6-4 of your technique book provides a detailed description of how to run a ion- exchange column. Assume you have a cation-exchange column already prepared and ready to use. Create an outline of no more than 10 steps describing how you will regenerate the column‚ load your sample and collect the hydronium ion released. 1. Open the stopcock at the bottom of the column and allow solution to drain out until the solution level in the
Premium Hydrogen PH Erlenmeyer flask
g A. Ion exchange Chromatography Ion exchange chromatography is a process for separating proteins and other molecules in a solution based on differences in net charge. Ion Exchange Chromatography relies on charge-charge interactions between the proteins in your sample and the charges immobilized on the resin of your choice. Ion exchange chromatography can be subdivided into cation exchange chromatography‚ in which positively charged ions bind to a negatively charged resin; and anion exchange chromatography
Premium Electric charge Ion Molecule
Determination of Percent Potassium & Percent Iron in an Iron Oxalate Salt by Ion Exchange Introduction: This experiment involves determining both the percent potassium (K) and iron (Fe) in a single titration after passing a solution containing a known mass of complex salt through an ion exchange column. Ion Exchange: Certain materials called ion exchange resins consist of rather large molecules which contain ions that can be displaced. The resins are solids‚ insoluble in water‚ usually granular
Premium PH Water Titration
Important Ions in the Body and the Roles They Play Everyday Functions of the Human Body The human body uses many forms of energy to conduct daily activities. These activities can be ones that we are doing physically or they can be as basic as the breakdown of enzymes within our body or the digestion of food. Chemical energy‚ electrical energy‚ mechanical energy‚ and electromagnetic energy are a few but important forms of the type of energy utilized by the human body. Ions play an important
Premium Electric charge Ion Electron
Importance of Calcium Ions Alyssa Foels 12 February 2015 Kilfe Calcium is a cation‚ a positively charged atom‚ and it is essential to all living things. It was first discovered in 1808 by Humphrey Davy. When forming an ionic bond with other elements‚ calcium loses electrons to make its valence shell stable. This means that it has more protons than electrons‚ making it have a slight positive charge (Calcium). Calcium has many roles and functions in the human body. Calcium ions are the most abundant
Premium Bone Ion Calcium
Reactivity of Halide Ions PURPOSE The purpose of this experiment is to observe the reactions of halide ions with different reagents by mixing them together. Materials 0.1 M AgNO3 0.1 M NaCl 0.1 M NaF 0.2 M KBr 0.2 M Na2S2O3 3% Starch Solution 4.0 M NH3 (aq) 5% NaClO (commercial bleach) 24 – well microplate thin – stemmed pipettes‚ 12 Safety DATA |PARTS |INITIAL MASS |FINAL MASS |CHANGE IN MASS | |
Premium Chemistry Chemical reaction Hydrogen
Ocean County College Department of Chemistry Identification of Metallic Ions Purpose: The purpose of this lab exercise was to perform and observe the flame tests of some alkali and alkaline earth metal ions. When electrons in metallic ions are grounded‚ their configuration is stable. In other words‚ the loosely bound electrons in the valence shell of the atom are satisfied at their “low” energy state. They do not require to be balanced by emitting any type of radiation or receiving any
Premium Potassium Metal Alkaline earth metal
MECH A NIS MS OF D IS EASE Review Article Mechanisms of Disease F R A N K L I N H . E P S T E I N ‚ M. D. ‚ Editor ION CHANNELS — BASIC SCIENCE AND CLINICAL DISEASE AND MICHAEL J. ACKERMAN‚ M.D.‚ PH.D.‚ DAVID E. CLAPHAM‚ M.D.‚ PH.D. I ON channels constitute a class of proteins that is ultimately responsible for generating and orchestrating the electrical signals passing through the thinking brain‚ the beating heart‚ and the contracting muscle. Using the methods of molecular biology
Premium Action potential Cystic fibrosis
of electrons. 2. An atom can NEVER gain or lose protons 3. The number of protons equals the atomic number NAME:________________________________________ Ion Practice Set 1. What is an ion? 2. What does the number next to the ions signify? Complete the following table‚ using the periodic table in the back of your book. ELEMENT NAME ION SYMBOL NUMBER OF PROTONS NUMBER OF ELECTRONS NUMBER OF ELECTRONS LOST OR GAINED ex Fluorine F- 9 10 gained one 1 2 3 4 5 6 7 8 9 10 11
Premium Atom Atomic number Chemical element
Ion Exchange Chromatography Discussion: The first exercise preformed in this lab was ion exchange chromatography. The purpose of this experiment is to separate molecules based on their differences in charge. Since it is based on charge the amino acids in the cation exchange column‚ if negatively charged‚ flow through the column first because they don’t want to bind to the sodium ions. The positively charged ions will elute last at the highest ph because they bind to the negatively charged beads
Premium Electric charge Ammonia Ion