Effect of Protein Intake on Strength‚ Body Composition and Endocrine Changes in Strength/Power Athletes Introduction: Proteins are often called the building blocks of the body. Protein consists of combinations of structures called amino acids that combine in various ways to make muscles‚ bone‚ tendons‚ skin‚ hair‚ and other tissues. They serve other functions as well including nutrient transportation and enzyme production. Adequate‚ regular protein intake is essential because it isn’t easily
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PROTEIN TRANSLATION 1. Initiation Translation To begin translation: large and small ribosomal subunit‚ along with the initiating tRNA‚ assembles onto the mRNA 2. Translation Elongation The next amino acyl-tRNA enters the ribosome at the A site The amino acid at the P site is transferred to the tRNA at the A site. The ribosome moves one codon farther along the mRNA‚ releasing the empty tRNA through the E (exit) site. The A site is free for the next incoming RNA. The cycle is repeated as the
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after the anion exchange chromatography was done and the fractions were collected. The picture of BCIP spot test of the 20 fractions can be seen in figure 1. The intensity of each spot was different from each other. The more intense color the spot was‚ the more concentrate the AP was. The fractions were chosen to combine together based on the intensity‚ which made the stage 4 enzyme. Different volumes in each stage were recorded. The volume could be seen in figure 2. The volume of protein decreased in
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Chromatography serves mainly as a tool for the examination and separation of mixtures of chemical substances. Chromatography is using a flow of solvent or gas to cause the components of a mixture to migrate differently from a narrow starting point in a specific medium‚ in the case of this experiment‚ filter paper. It is used for the purification and isolation of various substances. There are two phases in chromatography: 1. Stationary Phase – a solid that does not move. In this experiment was the
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Purification of Water Domestic: Distillation - In the process of distillation water is heated and evaporation takes place‚ whereby water changes back to water when cooled; this process is called condensation. The condensed water is the purest form of water‚ free from microbes‚ and impurities. Boiling - Boiling is the best and simplest method to purify water. After straining the water twice through clean muslin cloth‚ it is boiled for ten minutes lo kill the microbes present in it and also remove
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Chromatography is a process used to separate mixtures. The word chromatography is derived from the Greek words ‘khroma’ and ‘graphein’ meaning ‘color’ and ‘to write’ or to represent. Although there are a couple different types of chromatography‚ in each case a substance is placed onto or into a medium and a solvent is passed through the test substance. In chromatography science‚ the solvent is called the mobile phase or the carrier fluid and the medium is called the stationary phase. There are four
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Assignment Purification of a Mixture INTRODUCTION Mixtures obtained from the biosphere‚ lithosphere‚ hydrosphere or atmosphere can be industrially separated into their constituents. Petrol for example is obtained from crude oil found in the lithosphere. RESEARCH SKILLS When researching this assignment you will access a variety of secondary resources including web sites and textbooks. Please include a detailed bibliography You should also consider both the validity and reliability of your
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Size Exclusion Chromatography Size Exclusion Chromatography (SEC) is the separation technique based on the molecular size of the components. Size exclusion chromatography is a kind of method to separate different size of molecules that put in solution. It was first discovered by two scientists who named Grant Henry Lathe and Colin R Ruthven. Both of them received the John Scott Award for this fabulous invention. There are various applications for Size exclusion chromatography such as biochemical
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Column Chromatography of Plant Pigments Paul Ibarbia‚ Gene Paolo Jasmin‚ Gianpaolo Jimenez and Lorenzo Labicane* Department of Biology‚ University of Santo Tomas‚ Manila‚ Philippines Abstract Column chromatography of plant pigments is the separation of plant pigments extracted from Capsicum frutescens (siling labuyo). No slurry is prepared of the eluent; eluent with the stationary phase is set and then carefully poured into the column. The eluent are the solvents which are responsible for the
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PURIFICATION OF LACTATE DEHYDROGENASE FROM CHICKEN MUSCLE TISSUE Abstract The enzyme lactate dehydrogenase (LDH) catalyzes the last step of anaerobic glycolysis that is important for the normal function of the body. Purification of LDH is essential to understand its structure and function. The purpose of this experiment was to extract and purify LDH enzyme from chicken muscle tissue using a variety of various. Analytical methods such as activity and protein assay were employed to determine
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