Title of Lab: Preparation and Assay of Phenolase and Peroxidase from Sweet and Irish Potato Hypothesis: Polyphenol Oxidase enzyme activity can be detected by change in colour of solution‚ Inhibitors prevent the reaction of the enzymes with substrates‚ the enzyme is relatively specific. Aim: To design and conduct an experiment to demonstrate enzyme activity of Polyphenol Oxidase and Peroxidase when mixed with catechol‚ caffeic acid‚ pyrogallad‚ tyrosine‚ guacol‚ and water‚ to test the effect of
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Clinical Chemistry Manlangit‚ Joyce Ann U. EXPERIMENT Title: Standard Curve Preparation Objectives: 1. Properly set up a standard curve 2. Using the prepared curve‚ determine the concentrations for control and patient specimens. Materials and Reagents: 2ml 1M Sulfuric Acid 2ml of 0.1M Potassium Dichromate Distilled Water Pipettes Volumetric Flask Test Tubes Procedure: 1. Prepare 1 ml glucose solution and place in test tubes 2. Add 2ml of 1M Sulfuric Acid solution 3. Add
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EXERCISE 8-A: Preparation of Bacterial Smear and Simple Staining I. INTRODUCTION Bacterial smears are prepared for the purpose of viewing microorganisms under the microscope. Visualization of microorganisms in the living state is very difficult‚ not just because they are minute‚ but because they are transparent and almost colorless when suspended in an aqueous medium. A bacterial smear is a dried preparation of bacterial cells on a glass slide. Smears may be made from a dry culture (from a slant‚
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CHOONG SHING YI CHOONG SHING YI Preparation and Recrystallization of Aspirin Preparation and Recrystallization of Aspirin Date of the experiment: 9 May 2013 Date of submission: 23 May 2012 Date of the experiment: 9 May 2013 Date of submission: 23 May 2012 Synopsis 1. General Objective: To familiarize with the preparation of some simple organic compound and purify the compound by recrystallization. Specific Objective: The experiment is carried
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Preparation of 4-bromoaniline Introduction Aromatic compounds tend to undergo electrophilic aromatic substitutions rather than addition reactions. Substitution of a new group for a hydrogen atom takes place via a resonance-stabilized carbocation. As the benzene ring is quite electron-rich‚ it almost always behaves as a nucleophile in a reaction which means the substitution on benzene occurs by the addition of an electrophile. Substituted benzenes tend to react at predictable positions. Alkyl groups
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Educational Preparation in Nursing Grand Canyon University: NRS 430 11/11/2012 Educational Preparation in Nursing With a consistent change in modernizing medicine‚ along with the continuing advancement in technology‚ continuing education in nursing is essential for a variety of reasons. The nurse’s main concern is providing safe‚ efficient‚ and effective patient care with positive patient outcomes. This paper will examine the differences in competencies between nurses prepared at an associate-degree
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Education Preparation Paper Osaginwen K. Ayiyi Grand Canyon University: NRS 430v November 24‚ 2013 Education Preparation Paper There has always been an on going debate in health care‚ as to where the entry into nursing should be‚ this debate continues to divide the field of nursing and has prevented nurses from becoming a more dynamic and united profession (Spencer‚ 2008). The argument that more education translates into better performance is constantly debated. Because there are many
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The Preparation of 1- Bromobutane Introduction... Aim : To prepare 1-Bromobutane. Background : The most common way of preparing alkyl halides‚ which are very useful intermediates in syntheses‚ is the replacement of the OH group of an alcohol by a halogen. This replacement is a nucleophilic substitution reaction‚ and alcohols do not undergo nucleophilic substitution reactions because hydroxide ison is strongly basic and a poor leaving group. However‚ alcohols readily undergo
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adopted milk and its food products in their diets. Milk can be defined as the fresh and clear lacteal secretion practically free from colostrums and obtained by the complete milking of one or more milky animals like cow‚ buffalo‚ goat etc. In human beings milk is consumed as supplementary food for lifelong. Traditionally‚ milk was used to be collected directly from the milk producing animals like cow‚ buffalo etc. which were reared by individuals or looked after by a special class of people
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NAME: Maniwang‚ Ma. Aiza C. DATE: March 21‚ 2011 COURSE: BS Chemistry II APPROVED: ___________ PREPARATION OF AZO DYES ABSTRACT In this experiment‚ the azo dyes p-nitrobenzene azoresorcinol and methyl orange were prepared by the azo coupling reaction. The p-nitrobenzene azoresorcinol dye was prepared from p-nitroaniline and resorcinol. The diazonium salt formed was from the reaction of the cold solution of dissolved p-nitroaniline in hydrochloric acid and water with the solution
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