RNA translates it into protein and expressing it as a trait. Recombinant plasmids are when DNA fragments are inserted into a plasmid vector. The recognition site is where the plasmid gets cut by the restriction enzyme which is an enzyme that cuts a DNA molecule. Recombinant DNA is the DNA being inserted into the E.Coli. The purpose of the lab is to see if we can make the E.Coli glow and resistant to ampicillin. In the lab we were transforming a north american jellyfish by the name Aequorea victoria
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Glowing Transformations Abstract In this experiment‚ the idea is to become familiar with the transformation of cells. A well thought out procedure‚ involving a heat shock procedure‚ a good antibiotic‚ an inducer known as arabinose to show the newly expressed DNA by a visible fluorescent glow‚ and a stable control group is what contributes to this experiments thoroughness. It is predicted that the four agar plates will all yield different forms of growth‚ with different coloration and colony
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The pGLO plasmid‚ which has the bla and GFP gene‚ was developed so it can operate like the arabinose operon and be able to transcribe genes in the presence of arabinose sugar. In this plasmid‚ the cluster of genes is regulated by the spontaneous on/off element by a single promoter‚ which is dependent on the DNA binding protein araC. This protein is at the binding site for RNA polymerase at the beginning of the operon‚ so when arabinose is present (like in one of the experimental plates)‚ it is taken
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uses of plasmids in G.M. experimentation. Plasmids are extrachromosomal genetic elements found in a variety of bacterial species. They are double stranded; autonomously replicating‚ supercoiled‚ covalently closed circular (ccc) DNA molecules that range in size from 1 kb to greater than 200 kb. Often‚ plasmids contain genes coding for enzymes that‚ under certain circumstances‚ are advantageous to the bacterial host (Table 1). Table 1. Some of the phenotypes conferred by different plasmids that
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LSM1102 Lab Report Introduction Transformation is a process which involves plasmid DNA being bound to the cell surface and the subsequent uptake of DNA by the cell (Panja et al.‚ 2008). For artificial transformation of E. coli cells with plasmids‚ plasmid DNA has to be extracted from bacterial cells using the High-Speed Plasmid Mini Kit‚ which is then mixed with competent E. coli cells followed by heat shock and the streaking of transformed cells on two different types of agar plate (LB and LB+ampicillin)
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I. Title Identification of an Unknown Plasmid In this experiment‚ we determined the phenotypic capability of an unknown plasmid along with its size. With the use of gel electrophoresis‚ we analyzed the gel photograph by using a standard DNA marker‚ Lambda HindIII‚ and came to a conclusion based on our results. II. Abstract Two experiments were done to identify an unknown plasmid. The success of these experiments came from the use of modern day technology involving gel electrophoresis
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BIOL 1121: General Biology II Lab Fall 2014 Abstract Mark and recapture is a method commonly used in ecology to estimate an animal population ’s size. A portion of the population is captured‚ marked‚ and released. This lab provides methods that can be used to estimate a provided additional information for a better interpretation of lichen diversity values in biomonitoring studies of air pollution. Introduction This section is an introduction to the lab objectives and its practical uses
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Transformation of E. coli by plasmid DNA 1. Table showing the results from the selective plates |Plate |Plasmid |Contents of plates |Number of colony | | | | |White |Blue | |1 |Ligation mixture |Ampiclillin + X- gal + IPTG |10 |0
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cheese. Lactic acid bacteria(LAB)‚ a bacteria that can be found in the production of cheese‚ its stress gene was investigated in the experiment by using various biochemical and genetic techniques to identify and extract. The characterisation of the strain illustrates how identification of strains differ using different methods‚ such as gram stain and 16s rRNA screening. After the characterisation‚ the stress gene isolation assist the further understanding of the gene on LAB be giving different stress
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Abstract Some bacteria are able to go through transformation making new combinations of genes. Transformation is a way of gene variability in bacteria. This experiment is based on the transformation mechanism of bacteria and gene regulation. The bacteria used for the experiment was Escherichia coli and the genes introduces for the transformation were: gfp and bla by a pGLO™ plasmid. After the insertion of the target genes and growing the bacteria on specialized LB media‚ it could be seen that the
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