Determining absorbance of various wavelengths of light for pigments present in Coleus plants Joseph Yung (King Yung) 212831426 Adrian Ionescu Section M 11 February 5‚ 2014 Absorbance Table Absorbance Spectra Figure 1: Absorption spectra of pigments found‚ through chromatography‚ within Coleus plants. The different wavelengths of light were determined by the use of a spectrophotometer Questions
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Protein fragment showing peptide bond Basis of Spectrophotometer Measuring amount of substance in solution by helping of solution color is called colorimetry. Devices that used for this method is called colorimeter. In colorimetric measurements‚ color of solution is compared with color of standard solutions in different concentrations. Measuring amount of substance in solution by helping intensity of transmittance light from solution is called photometry. Devices that used for this
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Results: Part 1: Determination of Amax of bromophenol blue The wavelength with maximum absorbance reading form the graph is 590nm. Part 2: The effect of concentration on absorbance of bromophenol blue solution Tube 1 2 3 4 5 6 Distilled water (ml) 2.5 2.0 1.5 1.0 0.5 0.0 Bromophenol blue 10 mg/L (ml) 0.0 0.5 1.0 1.5 2.0 2.5 Concentration of bromophenol blue (mg/L) 0.0 2.0 4.0 6.0 8.0 10.0 Absorbance at 590 nm (Amax of bromophenol blue) 0.000 0.135 0.199 0.404 0.596 0.724 From Figure 2 above
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Experiment 2: Absorbance and Spectrophotometry ABSTRACT: This was an investigation into the effects of different wavelengths of light on methylene blue and carmine red on the absorbance value on a spectrophotometer. A spectrophotometer is used to measure light intensity by emitting a single light source through a cuvette of coloured solution. The particles in the solution‚ which are coloured‚ absorb the light depending on how concentrated it is and this produces an electronic reading from
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The purpose of this lab was to use a spectrophotometer to find the concentrations of glucose in varying samples of Gatorade. The varying executions included changing the temperature of reaction (room temperature instead of 40 degrees centigrade)‚ changing the time for reaction (20 minutes in the water instead of 45 minutes)‚ and changing the amount of enzyme added (0.1mL of enzyme. The results in from this lab gave data points with respect to ferricyanide. Fortunately‚ ferricyancide and glucose have
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recorder would read and record the absorbance from the spectrometer at the 5 second intervals. This was done for all four experiments so the absorbance could be recorded every 5 seconds for 60 seconds. It was highly important to keep in mind that all the tubes that would enter the spectrophotometer are clean and dry before the substrate and buffer was added. In the first experiment‚ optimal temperature for enzymatic activity was tested. Five clean spectrophotometer tubes wereare necessary with the
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Technical Specification Sheet For UV-Spectrophotometer | | Wavelength range | 190 to 1‚100 nm | Spectral bandwidth | 1 nm (190 to 1‚100 nm)/ variable Low stray light < 0.12%T (220 nm‚ NaI 340nm‚ NaNO2>2.0 Abs) | Wavelength accuracy | ± 0.1 nm at D2 peak 656.1 nm‚ | | ± 0.3 nm for entire range | Reproducibility | ±0.1 nm | Resolution | 1 nm more than 6 wave lengths | Photometric system | Double beam optics | Photometric range | Absorbance: -4 to 4 Abs | | Transmittance:
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report investigates the absorbance of methylene blue and carmine red using a spectrophotometer to determine the absorption spectra of both solutions. The concentration of the unknown solution of methylene blue was found to be 1.07 x 10-5 M by using the molar extinction coefficient‚ with absorption of 0.547. It was also found that the results concluded confirmed beer’s law with an R2 value of 0.9989. Introduction Spectrophotometry is the quantitative measurement of the absorbance or transmittance properties
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: Joanne Wong Student ID : 00000012636 (BM1/14) Title : Spectrophotometer and its function Introduction Spectrophotometry is the quantitative measurement of the reflection or transmission properties of a material as a function of wavelength.[1] It can measure any of the listed light ranges that usually cover around 200 nm - 2500 nm using different controls and calibrations. [1] There are a few types of spectrophotometer such as calorimeter‚ UV spectrometer‚ IR spectrometer‚ atomic spectrometer
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Discussion of Results and Scientific Explanation: In order to observe how much a chemical substance absorbs light a spectrophotometer can be used. A spectrophotometer measures the amount of light absorbed by measuring the difference in the intensity of the light before and after a beam of light passes through a sample solution. The area in which light is absorbed can provide information about the molecule‚ such as the concentration‚ by identifying the most preferentially absorbed wavelength.1
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