fluorescence test to decrease the no-specific binding. 2. Explain where the epitope (antigenic determinant) is located. The epitopes are located in the antigens for the antibodies binding. 3. Describe how a positive result is detected in this serological test. The elementary bodies of the Chlamydia trachomatis stains green inside the red host cell‚ and the presence of more elementary bodies in a field of view compared to the positive control is considered a positive result. 4. How would the
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Name ________________________ Date ________________ Endocrine System Physiology R E V I E W S H E E T E X E R C I S E 4 (Activity 2‚ 3‚ & 4) A C T I V I T Y 2 Plasma Glucose‚ Insulin‚ and Diabetes Mellitus 1. What is a glucose standard curve‚ and why did you need to obtain one for this experiment? Did you correctly predict how you would measure the amount of plasma glucose in a patient sample using the glucose standard curve? _A glucose standard curve is a method for identifying
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Danielle Rollins Cardiovascular Physiology Activity 1 1) Explain why the larger waves seen on the oscilloscope represent ventricular contraction. The ventricular contraction is when the blood is sent through the whole body as opposed to the atrial contraction that sends the blood to the heart and lungs. 2) Explain why the amplitude of the wave did not change when you increased the frequency of the stimulation. (Hint: relate your response to the refractory period of the cardiac action
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CD with the Lab exercises. PhysioEx 9.0 Lab Exercise text (REQUIRED) Author: Zao et al Text Publisher: Pearson Benjamin Cummings ISBN: 9780321905413 (text only) ISBN: 9780321929648 (text and CD package) ISBN: 9780321907127 (PhysioEx 9.0 with 9.1 updates -CD only) 3. If the PhysioEx 9.0 text is purchased alone – there is online access to the labs after the student registers following the instructions in the front of the text. If you purchase a used PhysioEx 9.0 text‚ there is a fee to
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Review Sheet Exercise 12 Serological Testing NAME Kali Rothfuss LAB DATE/TIME 6/22/11 Positive and Negative Controls 1. Why are there a number of washing steps in serological tests? The are a number of steps needed in order to remove any non specific binding that may have occurred. 2. Describe how you would know that you had a “false positive” result. What does this mean for the rest of your results? A positive result with a negative control indicates a “false positive”
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Neurophysiology of Nerve Impulses ACTIVITY 1 The Resting Membrane Potential 1. Explain why increasing extracellular K+ reduces the net diffusion of K+ out of the neuron through the K+ leak channels. If the extracellular K+ increases then the concentration of intracellular K+ will decrease causing a decrease in the steepness of the concentration gradient and fewer K+ ions would be drawn out. 2. Explain why increasing extracellular K+ causes the membrane potential to change to a less negative
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LAB ONE: Exercise 4 – Endocrine System Activity 1: Metabolism and Thyroid Hormone Part 1: 1. The normal rat had the fastest basal metabolic rate. 2. The metabolic rates differed between the normal rat and the surgically altered rats because the surgically altered rats didn’t have a thyroid or pituitary gland and therefore‚ the normal rat had a faster basal metabolic rate. The results turned out to be the way I predicted them to be. 3. T3 and T4 would be missing in the animal’s blood
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Exercise 12: Serological Testing: Activity 2: Comparing Samples with Ouchterlony Double Diffusion Lab Report Pre-lab Quiz Results You scored 100% by answering 4 out of 4 questions correctly. 1. When two antigens are identical their precipitin lines form You correctly answered: c. an arc 2. Antigen and antibody move toward each other because of You correctly answered: b. diffusion 3. If two antigens form a spur‚ they You correctly answered: d. have partial identity 4. The Ouchterlony test relies on
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ACTIVITY 1: THE RESTING MEMBRANE POTENTIAL 1. Explain why increasing extracellular K+ reduces the net diffusion of K+ out the neuron through the K+ leak channels? When the diffusion is greater on one side‚ the other side will slow down. 2. Explain why increasing extracellular K+ causes the membrane potential to change to a less negative value. How well did the results compare with your predictions? There are two potassium’s for every sodium so the increase of potassium will make it more
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EXERCISE 9: RENAL SYSTEM PHYSIOLOGYHere is 9 got 95% Still need help on final 132 | ACTIVITY 1: The Effect of Arteriole Radius on Glomerular Filtration Answers 1. According to your lab manual‚ in humans the glomerular filtration rate (GFR) ranges from _____ to _____ ml/minute. 80 140 2. When the radii of both the afferent arteriole and efferent arteriole were set at 0.45mm‚ the GFR was approximately ______ ml/minute. a. 40 b. 60 c. 80 d. 120 C. 3. True or
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