PHYSIOEX 9‚ ACTIVITY #3 Review Questions ACTIVITY #1 1. Explain why increasing extracellular K+ reduces the net diffusion of K+ out the neuron through the K+ leak channels? 2. Explain why increasing extracellular K+ causes the membrane potential to change to a less negative value. How well did the results compare with your predictions? 3. Explain why a change in extracellular Na+ did not significantly alter the membrane potential in the resting neuron? 4. Discuss the relative permeability
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Exercise 3: Neurophysiology of Nerve Impulses Worksheet Assignment Due: Week 4 Student instructions: Follow the step-by-step instructions for this exercise found in your text and record your answers in the spaces below. Submit this completed document by the assignment due date found in the Syllabus. Eliciting a Nerve Impulse Activity 1: Electrical Stimulation 1. Do you see any kind of response on the oscilloscope screen? Just a flat line. 2. What was the threshold voltage
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results compare with your prediction? Your answer: If extracellular K+ is increased then the intracellular K+ will decrease. Fewer intracellular K+ ions would result in the membrane potential being less negative. This is what I predicted to happen. 3. Explain why a change in extracellular Na+ did not significantly alter the membrane potential in the resting neuron? Your answer: There are less sodium leak channels than potassium leak channels‚ and more of the potassium channels are open. 4. Discuss
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Activity 1 Pre-Lab Quiz K+ inside a cell = 150 mM; K+ outside a cell = 5 mM Na+ inside a cell = 5 mM; Na+ outside a cell = 150 mM Stop & Think question What is the polarity of the resting membrane potential (voltage)? Negative What does it mean that the voltage just inside the membrane is negative? B Membrane of most cells contains K+ leak channels…..b What will happen to the resting membrane potential if the extracellular K+ concentration is increase? Less negative What happens
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Activity 1 1. Increasing extracellular K+ reduces the net diffusion of K+ out of the neuron through the K+ leak channels because the membrane is permeable to K+ ions. Therefore‚ the K+ ions will diffuse down its concentration gradient from a region of higher concentration to a region of lower concentration. 2. Increasing extracellular K+ causes the membrane potential to change to a less negative value because the K+ ions diffuse out across the membrane. My results went well compared to my prediction
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Activity 1: Electrical Stimulation 1. Do you see any kind of response on the oscilloscope screen? NO 2. What was the threshold voltage‚ or the voltage at which you first saw an action potential? 3.0 V 3. How does this tracing compare to the one that was generated at the threshold voltage? The tracing travel in the same fashion but the tracing were different with an increase threshold voltage 4. What reason can you give for the change? Increase voltage 5. Record this maximal voltage here:
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Neurophysiology of Nerve Impulses ACTIVITY 1 The Resting Membrane Potential 1. Explain why increasing extracellular K+ reduces the net diffusion of K+ out of the neuron through the K+ leak channels. If the extracellular K+ increases then the concentration of intracellular K+ will decrease causing a decrease in the steepness of the concentration gradient and fewer K+ ions would be drawn out. 2. Explain why increasing extracellular K+ causes the membrane potential to change to a less negative
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ACTIVITY 1: THE RESTING MEMBRANE POTENTIAL 1. Explain why increasing extracellular K+ reduces the net diffusion of K+ out the neuron through the K+ leak channels? When the diffusion is greater on one side‚ the other side will slow down. 2. Explain why increasing extracellular K+ causes the membrane potential to change to a less negative value. How well did the results compare with your predictions? There are two potassium’s for every sodium so the increase of potassium will make it more
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Exercise 3: Neurophysiology of Nerve Impulses Worksheet Assignment Due: Week 4 Student instructions: Follow the step-by-step instructions for this exercise found in your text and record your answers in the spaces below. Submit this completed document by the assignment due date found in the Syllabus. Please make sure that your answers are typed in RED. Please type your Name and Student ID Number here: Charles Coon 4048223 Eliciting a Nerve Impulse Activity 1: Electrical Stimulation
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Depolarization has to do with surfaces becoming more negative or positive and reaches a certain point called a threshold. When the threshold is reached‚ an action potential is initiated. 2. What was the threshold voltage in Activity 1? The threshold voltage in Activity 1 was 3.0. 3. What was the effect of increasing the voltage? How does this change correlate to changes in the nerve? There was little change in effect of increasing the voltage. The action potential increased slightly. There was a
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