Experiment 2 : Transport Across Membrane Name : Matrix No : Group : B Semester : 1 Date of Experiment : 05.09.2013 Lecturer’s Name : Miss OBJECTIVE To study the effects of hypotonic‚ hypertonic and isotonic solutions on plant and animal cells. INTRODUCTION In cellular biology the term membrane transport refers to the collection of mechanisms that regulate the passage of solutes such as ions and small molecules through biological membranes‚ which are lipid bilayers
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3.1 BIOLOGICAL MEMBRANES 3.1.1 Properties of Cell Membranes • Separates living cell from its nonliving surroundings. • 8 nm thick. • Selectively permeable - allows some substances to cross more easily than others. 4.1.2 Fluid Mosaic Model • Singer and Nicolson (1972) - plasma membrane is a mosaic of proteins dispersed within lipid bilayer‚ with only bilayer‚ the hydrophilic regions exposed to water. Hydrophilic region of protein Phospholipid bilayer Hydrophobic region of protein
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1 Experiment Membrane Transport Objectives ► Referring to energy‚ what two ways can substances enter a cell? What is active transport? What is passive transport? How is osmosis related to diffusion? How can we demonstrate active transport? How can we demonstrate Brownian movement? How can we demonstrate diffusion (2 ways)? How can we demonstrate osmosis (3 ways)? In terms of relationships between substances‚ how can we define “hypertonic”‚ “isotonic”‚ and “hypotonic”? What is the relationship
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Cell Membranes and Transport Hands-On Labs‚ Inc. Version 42-0033-00-01 Exercise 1: Diffusion Observations Data Table 1: Rate of diffusion in different temperatures | | ºC | Minutes | Temperature | InitialTemp. | InitialColor | 5 | 10 | 15 | 20 | 25 | 30 | 60 | Cold | 10°celsius | clear | clear | clear | clear | clear | clear | light purple | light purple | Ambient | 25°celsius
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Transport Across The Plasma Membrane Intracellular Fluid (ICF) – The two thirds of your body fluid contained inside body cells. (Intra = within). The cytosol of the cell. Extracellular Fluid (ECF) – Fluid outside the body cells. (extra = outside). Interstitial Fluid – The ECF in tiny spaces between cells (inter = between). Plasma- the ECF in blood vessels. Lymph- The ECF in lymphatic vessels. Solute – Any material dissolved in fluid. Solvent- The fluid a Solute is dissolved in. Concentration
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Cell Membrane Transport The purposes for these experiments is to be able to understand osmosis and its relationship to tonicity of solutions‚ and the transportation of molecules across cell membranes. It’s also used to understand‚ and how temperature affects diffusion. You also learn how to test for presences of starches and sugars in solutions. In the first exercise I will be testing for diffusion through an artificial membranes. This one has forty five steps to it. I will not go into
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this Lab the students dealt with Osmosis‚ movement of water molecule or solvent from a high concentration to a low concentration‚ through selective permeability‚ a protective barrier that provide some particle the ’direct ’ passage in and out of the plasma membrane ( Lacerda L.2011) Allowing some molecules enter and exit the cell membrane‚ to create a balance in concentration inside and outside of the cell‚ by doing so the cell often become hypotonic‚ where the concentration outside the cell is higher
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BioLab3 Cell Membranes Lab Report Student Name: I. Diffusion Define the following terms. Solvent Solute Solution. Diffusion Concentration gradient Dynamic equilibrium EXERCISE 1 – Factors influencing rate of diffusion Predict how molecule size and temperature will affect the outcome of this experiment. Record the data from the information in the lab. Potassium Permanganate R.T. Methylene Blue R.T. Time (min) Total Diameter
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Experiment 2 : Transport Across Membrane Name : Umi Adiba Anis Binti Che Hamid Matrix Number : CPM0147_2013B Batch : Pre-Med May Intake 2013 Group : E Date : 19.06.2013 Lecturer’s Name : Miss Nurul Izziani Objectives : I. To study the effects of hypotonic‚ hypertonic‚ and isotonic solutions on plant and animal cells. Experiment 1 : Introduction : The purpose of doing this experiment is to investigate the effects of hypotonic‚ hypertonic‚ and isotonic solutions on plant cell. Particularly
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Testing Cell Transportation Across a Membrane Introduction Cells have the amazing ability to transport certain molecules in or out of their membrane. Some require no energy to do so (passive transport) while others require energy to be processed through (active transport). There is also the transportation of water across a membrane‚ which has its own term of osmosis. Too much of something can be taken in‚ or too little enters. This especially happens to plants‚ who require water (and sun) to live
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