Lab Report Tansport Across Membrane
Biology Experiment 2 :
Transport Across Membrane
Name : Umi Adiba Anis Binti Che Hamid
Matrix Number : CPM0147_2013B
Batch : Pre-Med May Intake 2013
Group : E
Date : 19.06.2013
Lecturer’s Name : Miss Nurul Izziani
Objectives : I. To study the effects of hypotonic, hypertonic, and isotonic solutions on plant and animal cells.
Experiment 1 :
Introduction :
The purpose of doing this experiment is to investigate the effects of hypotonic, hypertonic, and isotonic solutions on plant cell. Particularly in this experiment we use onion cells. Tissue from an onion is a good first exercise in using the microscope and viewing plant cells. The cells are easily visible under a microscope and the preparation of a thin section is straight forward. An onion is made of layers, each separated by a thin skin or membrane. In this exercise you will make a wet mount on a microscope slide and look at the cells of the onion membrane magnified by the high power, compound microscope. We also can observe the actual structure of plant cells which consists of nucleus, vacuole, cytoplasm, cell wall etc. This experiment indicates the transport across membrane in plant cells when involving water through osmosis.
Materials and methods :
Materials | Apparatus | Onion Distilled waterTable sugar/sucrose | Small knife Glass slideCover slipMicroscope Filter paperMagnetic stirrer/stirring rodSpatula Beakers (250 ml & 500 mi) |
I. Scale off carefully the epidermal layer of an onion and place it on a drop of distilled water on the glass slide. II. Lower down the glass cover slowly on the epidermal layer using the needle. III. Examine the onion cells through the microscope. IV. Draw the structure of the onion cells as observed under microscope. V. Remove the distilled water using filter paper. VI. Place a drop of 5% (w/v) sucrose solution at a side of the cover slip and draw the solution across the epidermal layer by placing filter paper on the other
Transport Across Membrane
Name : Umi Adiba Anis Binti Che Hamid
Matrix Number : CPM0147_2013B
Batch : Pre-Med May Intake 2013
Group : E
Date : 19.06.2013
Lecturer’s Name : Miss Nurul Izziani
Objectives : I. To study the effects of hypotonic, hypertonic, and isotonic solutions on plant and animal cells.
Experiment 1 :
Introduction :
The purpose of doing this experiment is to investigate the effects of hypotonic, hypertonic, and isotonic solutions on plant cell. Particularly in this experiment we use onion cells. Tissue from an onion is a good first exercise in using the microscope and viewing plant cells. The cells are easily visible under a microscope and the preparation of a thin section is straight forward. An onion is made of layers, each separated by a thin skin or membrane. In this exercise you will make a wet mount on a microscope slide and look at the cells of the onion membrane magnified by the high power, compound microscope. We also can observe the actual structure of plant cells which consists of nucleus, vacuole, cytoplasm, cell wall etc. This experiment indicates the transport across membrane in plant cells when involving water through osmosis.
Materials and methods :
Materials | Apparatus | Onion Distilled waterTable sugar/sucrose | Small knife Glass slideCover slipMicroscope Filter paperMagnetic stirrer/stirring rodSpatula Beakers (250 ml & 500 mi) |
I. Scale off carefully the epidermal layer of an onion and place it on a drop of distilled water on the glass slide. II. Lower down the glass cover slowly on the epidermal layer using the needle. III. Examine the onion cells through the microscope. IV. Draw the structure of the onion cells as observed under microscope. V. Remove the distilled water using filter paper. VI. Place a drop of 5% (w/v) sucrose solution at a side of the cover slip and draw the solution across the epidermal layer by placing filter paper on the other
References: : 1. Biology lab manual 2. http://chemistry.about.com/od/imagesclipartstructures/ig/Science-Clipart/Osmosis---Blood-Cells.htm 3. Campbell Biology, Ninth Edition, Pearson Publication, 2011, Reece, Urry, Cain, Wasserman, Minorsky, Jackson.