to remove a large majority of the impurities whicn include viruses and media components. At Stage 3‚ prior to cation exchange chromatography‚ the purified material in the eluted stream is then subjected to freezing‚ thawing and pooling. Stage 4 involves the Solvent/Detergent (S/D) viral inactivation which is the first dedicated viral clearance step. At Stage 5‚ the product undergoes cation exchange chromatography which aids in removing the S/D reagents while the product remains bound to the column
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References: 1. Nielsen‚ S. Suzanne. Food Analysis. Third ed. New York:Kluwer Academic/ Plenum Publisher‚ 105-110 (2003). 2. Pomeranz Y.‚and Meloan Cliffton E. Food Analysis: Theory and Practice. Third ed. New York: Chapman & Hall‚ 602-624 (2000). 3. Tee E Siong‚ Mohd Ismail Noor‚ Mohd Nasir Azudin‚ Khatijah Idris. (1997). Nutrient Composition of Malaysian
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Gravimetric Analysis of SULFATE as Barium Sulfate. Objectives * To learn the techniques associated with gravimetric analysis. * To use stoichiometry to calculate the percentage by mass of sulfate in an unknown sulfate salt. Background Gravimetric analysis is a quantitative method for accurately determining the amount of a substance by selective precipitation of the substance from an aqueous solution. The precipitate is separated from the remaining aqueous solution by filtration and
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Qualitative Analysis of Cations Short Overview The most common cations have been placed into five groups based upon solubility in aqueous solutions when different reagents are added. The reactions which occur are useful in identifying the presence of these cations in unknown samples. The process of identifying the cations is called qualitative analysis. The purpose of this experiment is to identify which cations are present in unknown solutions. A data sheet was not prepared for this experiment
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Arterial blood gases are only done in the very sick children‚ who have been intubated/ventilated and have arterial lines in situ (usually in the theatre prior to transfer to the PICU in Newcastle. Here is a stepwise approach to blood gas analysis. STEP 1: [pic] STEP 2: Look at pCO2 [pic] STEP 3: Look at Bicarbonate & Base excess (BE) [pic] Base excess (BE): Base excess is the amount mmol/l of base that is needed to correct pH back to normal when pCO2 is corrected
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Gravimetric Analysis CHEM 200-03 Fall Semester 2012 September 19‚ 2012 Jeff Marasigan Abstract Gravimetric analysis is based on the quantitative isolation of the desired constituent the analyte of interest from the sample in highly pure form or in some combined form and weighing the isolated constituent. The desired constituent is usually isolated or separated by precipitation. From the weights of sample and precipitate‚ the percentage of the constituent in the original sample can
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linen and spices. After this‚ the body would then be left to dry by using a salt (natron) mixture called a mummion which absorbed all the moisture from the body to dry it out‚ and then it was wrapped up in linen bandages. Mummion is an inorganic solid that is obtained from dried up lake beds and it used to mummify the dead bodies. I am going to explain what Ions are including Cations and Anions because it is the background information needed to carry out this investigation. An ion is an atom or molecule
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+ 4HCl(aq) Zn2+(aq) + 4H2O(l) + 4Cl-(aq) Post-Lab Questions: 1. What cation(s) are present in the unknown sample? How do you know? The cation(s) present in the unknown sample are silver and iron. I know this because the precipitates and the colors of the elements precipitates matched therefore making the unknowns identifiable. 2. Write a net ionic equation for each of the following steps in the qualitative analysis scheme. a) Separation of silver ions‚ Ag+ Ag + (aq) + HCl(aq) AgCl(s) +
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OBJECTIVE The main objective of this experiment is to carry out qualitative analysis to identify metal cations in unknown solution 1. INTRODUCTION An unknown material can be determined by using simple chemical tests and separations which is called as inorganic qualitative analysis. The separation of cations depends on the difference in their propensity to form precipitates. Separation scheme is used to classify cation into five groups on the basis of their physical and chemical behavior opposed
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References: 1. Kippenhan‚ Edith. Chem 1290 Laboratory Manual: Water Analysis. Cengage Learning. Mason‚ Ohio. 2012. 2. Procedure given to us in lab. 3. http://en.wikipedia.org/wiki/Total_dissolved_solids 4. http://www.watersystemscouncil.org/VAiWebDocs/WSCDocs/9709284pH_Update_September_2007.pdf
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