Introduction Diffusion is a process where the molecules intermingle as a result of their kinetic energy of random motion and is the net movement of like molecules down their concentration gradient‚ which is the energy inherent in their constant molecular motion makes them collide and careen outward from the region of higher to lower concentration. In our daily lives‚ many tasks are accomplished with the help of diffusion. For example‚ farting is considered to be diffusion‚ releasing a very
Premium PH Molecular diffusion Measurement
greater number of molecules or larger particles in the medium‚ the lower the rate of diffusion because these particles act as a barrier to diffusion. High concentration gradient leads to greater rate of diffusion considering the greater probability of molecular collisions. One of the main factors that affect the rate of diffusion is the temperature. The higher the temperature‚ the faster is the rate of diffusion because as temperature increases‚
Premium Molecular diffusion Diffusion Energy
What is gel electrophoresis? Gel electrophoresis is a technique that separates pieces of DNA (or other biological molecules) by size. Pieces of DNA in a test tube all look the same. DNA Gel Electrophoresis Gel electrophoresis separates pieces of DNA by size so that researchers can further analyze them BURST Training Session November 29‚ 2005 Once the DNA samples are loaded onto the gel‚ an electric current is applied to the gel. DNA is negatively charged due to all the phosphate groups in the
Premium Molecular biology
Introduction There are many different factors that can influence the rate of diffusion through a membrane. Chemical kinetics plays a large part in diffusion. In order for a solute to passively diffuse through a membrane‚ it must line up with a pore in the membrane and pass through it (textbook 101). The concentration gradient is also important for diffusion because solutes diffuse from areas of high concentration to areas of low concentration (textbook page 101). There are different factors that
Premium Osmosis Diffusion Chemistry
Material and methods Hosts ‚ plasmids and chemicals E. coli TOP10 strain was used for cloning and proliferation. E.coli BL21 DE3 was used as expression host cell. The pET 28a(+) plasmid was employed for gene expression. All chemicals were obtained from Merck Company (Germany). Codon optimization and gene synthesis Sequence encoding V-domain was obtained from Swiss-port‚ Uniprot KB and National Center for Biotechnology Information (NCBI) databases. 6x His-tag sequence was placed at N-terminal
Premium DNA Gene Molecular biology
Draft: Introduction: Transgenesis is the process by which new characteristic(s) can be introduced into an organism that it did not have previously. Exogenous gene(S)– called transgene- are introduced into an animal through the use of recombinant DNA technology. The extra genes or gene may come from same species or from a completely different species. Transgenesis has many beneficial applications for human application such as producing milk with human proteins‚ which help with treatment of
Premium Gene DNA Molecular biology
References: 1. Green E. (2014) 216 BMS Molecular Genetics: DNA Cloning Labs 1-3. 2. Brown‚ T. A. (2002) Genomes‚ 2nd ed. Oxford 3 4. Kerbs‚ J. et al. (2011) Lewin’s Genes X. 11th ed. London 5 7. Thermo Fisher Scientific (2014) Agarose gel electrophoresis tips & tricks [online]Available at https://www
Premium Molecular biology DNA Plasmid
an entry level by examining the hierarchy that ranges from the fundamentals of cell biology to the physiology of organisms‚ and the interactions among those organisms in their environment. The topics in this course include cell biology‚ genetics‚ molecular biology‚ evolution‚ physiology‚ and ecology. Policies Faculty and students/learners will be held responsible for understanding and adhering to all policies contained within the following two documents: • University policies: You must be
Premium Biology DNA Evolution
MRI for a patient can be challenging. This is because equipment is expensive‚ and image acquisition and interpretation are both time-consuming. Thus‚ it is relatively unavailable for acute imaging. However‚ as previously discussed‚ MRI is optimal in the acute setting. Magnetic resonance imaging of TBI in the subacute time frame can greatly assist in optimal patient management. Early comparative studies showed that brain CT failed to detect 10 to 20% of brain abnormalities visualized
Premium Magnetic resonance imaging Traumatic brain injury Medical imaging
Abstract This experiment followed an exact protocol from EDVOTEK® Kit #17. This kit was designed to use western blot analysis to detect proteins from Non-fat powdered milk. The experiment had to be run two times before the expected outcome was reached. However‚ after the second attempt‚ it was successful. The gel ran well‚ and the proteins from the gel were successfully transferred to the membrane provided in the kit. Introduction The name western blot was given to the technique by W. Neal
Premium Western blot Molecular biology Gel electrophoresis