Abstract This experiment is to study and measure the enzyme activity of β-galactosidase in the different concentrations of o-Nitrophenylgalactoside (ONPG) using a spectrophotometer. The spectrophotometer was also set at 420nm‚ a wavelength which is best for recording the absorbance values for the experiment. From the results‚ 0.9mM ONPG solution has the highest absorbance and 0.1mM ONPG solution has the least. Also‚ 0.5mM ONPG solution has the highest rate of enzyme activity and it is the most efficient
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Chemical Kinetic Models Simplified Global Chemical Kinetic Model In order to compare the two reactors used in our experiment‚ a simplified global kinetic model was used to describe the DRM reaction behavior in different plasma reactor. This model was already used by authors in the field of pollutant removal by using a plasma reactor [15‚ 21–23]. It is generally accepted that free radical processes are the main mechanisms in non-equilibrium plasma reaction [21‚ 24-25]. The chemical kinetics model
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CHEMICAL KINETICS RESULTS AND DISCUSSION The balanced equation of the reaction between sodium thiosulfate and Hydrochloric acid is: S2O32- (aq) + 2 H+ (aq) SO2 (g) + S (s) +H2O (l) Using beakers with the same diameter was very important in conducting the experiment. If different sizes were used‚ the visibility of the “x” on the paper beneath the beaker could disappear from view too early or too late than the hypothetical time depending on the depth of the solution. It was also significant
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The University of Lethbridge Department of Chemistry & Biochemistry Chemistry 2740 Laboratory Experiment 2 A KINETIC STUDY OF THE BASE CATALYZED CLEAVAGE OF DIACETONE ALCOHOL USING A DILATOMETER The decomposition of diacetone alcohol into two molecules of acetone is catalyzed by hydroxide ions and is an example of an aldol condensation in reverse. O OH OHO 2CH3-C-CH3 CH3-C-CH2-C(CH3)2 The rate of decomposition is first-order with respect to the concentrations of both diacetone alcohol
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Chemical Kinetics‚ A Clock Reaction Abstract The purpose for the experiment Chemical Kinetics‚ a clock reaction is to figure out the reaction rate of a solution regarding its concentration‚ temperature‚ and also determine the effects of when a catalyst is present. The experiment resulted that the concentration‚ as well as its temperature can affect the rate constant inversely the outcome of the rate constant. By conducting the experiment‚ it is also discovered that the rate order of the reaction
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Enzyme Kinetics Examples and Problems 1. An enzyme is produced for producing a sun protection lotion. Given kinetic data for the enzyme reaction with Vm=2.5 mmol/m3.s‚ Km=8.9 mM and So=12mM‚ what would be the time required for 95% conversion in a batch reactor? 2. An enzyme was assayed at an initial substrate concentration of 10-5M. The Km’ for the substrate is 2x10-3M. At the end of 1 min‚ 2% of the substrate had been converted to product. a. What % of the substrate will be converted
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momentum of the object over a long time D. Increase the momentum of the object over a long time 8. Most street barriers are made of hard rubber or plastic materials that easily give in or dent when hit. Why is this so? A. To reduce the vehicle’s kinetic energy when it collides with the barriers B. To store potential energy in the bumpers of vehicles C. To reduce the impact force when hit by a vehicle D. To reduce the impact time when the barriers hit the vehicle 9. What is the momentum of a 20
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types of forces involved. Two of the main forces experienced are called potential energy and kinetic energy. Potential energy is the energy an object has depending on the position of it. For example the higher an object is the higher the potential energy is and the lower an object is the less potential energy it will have. Kinetic energy is also a main type of force that is involved with rollercoasters. Kinetic energy is the energy an object will have as it is in motion. There are many different types
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EXPERIMENT 3-CHEMICAL KINETICS: THE IODINE-CLOCK REACTION J.CHAN1 and C.CABANLIG2 1NATIONAL INSTITUTE OF MOLECULAR BIOLOGY AND BIOTECHNOLOGY‚ COLLEGE OF SCIENCE 2NATIONAL INSTITUTE OF MOLECULAR BIOLOGY AND BIOTECHNOLOGY‚ COLLEGE OF SCIENCE UNIVERSITY OF THE PHILIPPINES‚ DILIMAN‚ QUEZON CITY 1101‚ PHILIPPINES DATE SUBMITTED: JANUARY 8‚ 2013 DATE PERFORMED: DECEMBER 5‚ 2012 ------------------------------------------------- ABSTRACT The kinetics of the reaction between persulfate (S2O82-)
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Experiment 16: Kinetics: The Iodine Clock Jane Smith Purpose: The objective of this experiment was for students to apply their knowledge of kinetics and rate laws in order to determine the rate of a chemical reactions‚ activation energy‚ and frequency factors of those reactions. Specifically‚ this experiment was performed using a series of solutions with varying concentrations of KI‚ Na2S2O3‚ and (NH4)2S2O3 . Students recorded time elapsed to observe physical evidence of a reaction taking place
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