Aim: To verify the buffering capacity of a phosphate solution using a pH meter and construct a titration curve of an amino acid to determine the pka values of its ionisable groups to identitfy an unknown amino acid. Method: The ratio of [HPO42-] to [H2PO4-] required to produce buffer solutions at pH values 5.9‚ 6.9 and 7.9 were calculated. 0.1M of H2PO4- and 0.1M HPO42- were used to mix appropriate volumes to 25mL of each of the buffer solutions. The calibrated pH meter was used to measure
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References: John R Holum‚ 1996‚ 2nd ed‚ organic and biological chemistry‚ von Hoffman press. Monique Laberge phd‚2008‚ 1st ed‚ essential chemistry‚ Chelsea house publications. Nelson David‚ 2005‚ 1st ed‚ principles of biochemistry‚ WH freeman company. www. Wikipedia.com? Cori /cycle Lowenstein J.M‚ 1969‚ 1st ed‚ citric acid cycle‚ Boston academic publication. Bhagavan et al‚ 2006‚
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In the introduction to “The Uses of Enchantment” by Bruno Bettelheim‚ he promotes reading fairy tales to children saying that they promote a child’s ability to find meaning in his or her life. He stresses the idea that fairy tales have certain qualities that do this that are not found in other children’s stories. Bettelheim employs sophisticated diction‚ punctuation in the form of dashes‚ and anecdotes from fairy tales to argue the importance of reading fairy tales to children. Bettelheim successfully
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Lab 2: Internet Resources for Protein Biochemistry Part 1-1: A) Protein Accession Number = P00939 Name of Protein: Full Name = Triosephosphate isomerase; Short Name=TIM Organism where the protein is found: Oryctolagus cuniculus = Rabbit Nuber of amino acids in the protein: AA = 248 B) * Amino Acid Sequence: >gi|136066|sp|P00939.1|TPIS_RABIT RecName: Full=Triosephosphate isomerase; Short=TIM;
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Summary of Introduction to the New Edition In Stuart Ewen’s All Consuming Images‚ the preface “Introduction to the New Edition” opens by giving the audience varying progressing images‚ from break dance to Madonna to Windows 95. This demonstrates a fast change in society: what matters in the history may not be an important issue now. Ewen then questions how a book written earlier still remains important and deserves republication. The book is durable because of the fact that it was written when the
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1) A peptide‚ KKDSRHSTTR is tightly bound to a negatively charged ion exchange column. The column is washed with a pH 7.2 HEPES buffer (50 mM) and the peptide does not elute. Suggest two ways that you can change the buffer that will make the peptide elute from the column. 2) What amino acid(s) is/are involved in crosslinking polypeptide chains. How can these crosslinks be cleaved and prevented from reforming? 3) A polypeptide is subjected to the following degradative techniques results
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Biochemistry of Photosynthesis Overall Word Equation: Water+ Carbon Dioxide( Carbohydrates+ Oxygen ((: light + chlorophyll) 1. Light Dependent Stage/ photochemical reaction/ light reactions: Word Equation: Water ( Hydrogen ions + Oxygen ((: light + chlorophyll) Site: On the Thylakoid membrane of the chloroplast Major events: • Light absorption o Chlorophyll in the thylakoids absorbs light. The
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1. Explain how carbon’s electron configuration and valence result in its ability to form large and complex organic molecules. These covalent bonds can be single‚ double‚ or triple bonds‚ but one carbon atom is limited to only 4 covalent bonds. It can bond with other carbon atoms and different atoms (H‚ O‚ N‚ P‚ S) to form long chains‚ ring shaped molecules‚ and branched/irregular shapes. 2. Explain why the combination hydrocarbon backbone +functional group(s) accounts for the unique functions
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Basic Biochemistry Webquest (2013) Basic Chemistry Part 1: Why Do Atoms React? Google Search 1. What is an atom? An atom is the basic unit of a chemical element. 2. What is an element? An element is a substance that is made entirely from one type of atom. 3. What makes an atom unstable (reactive)? The binding energy not being strong enough makes an atom unstable (reactive). 4. Why do atoms react with other atoms? Atoms react with other atoms to gain or lose electrons to fill their outer
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what I accomplish in the campus reflects my personality. I decided to study biochemistry because I want to make a change in this world‚ and also because I want to serve the humanity through harnessing the fields of biology and chemistry to ameliorate people’s health and to develop new technologies that will improve industrial processes. Possessing millions of dollars will support my post studies in chemistry and biochemistry branches‚ which will enhance my level of knowledge in these fields‚ thus having
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