MICROBIOLOGY 3444-007 Gram Negative Unknown Unknown Bacteria #14 LeNaiya Kydd 4/2/2014 Abstract In order to be able to identify the unknown organism that was given to us‚ we had to conduct a number of different tests. These biological tests are used because they help us be able to identify the properties of the unknown we have and be able to compare our observed results with actual results of all the potential organisms. When all of the data of the test are put together it is easy to
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obtain a pure culture of a Gram positive coccus genus of bacteria. Several biochemical tests will be performed to aid in the identification of this unknown bacterium. Biochemical tests are a series of tests used to identify certain bacterium The various tests that are used in this lab are the catalase test‚ oxidase test‚ blood hemolytic test‚ MSA‚ blood agar‚ and PEA/ab sens test. A known Gram positive coccus bacteria will be used alongside an isolated Gram positive unknown bacteria for comparison
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_____________________________________________ Project 3: Identifying the Unknown White Compound by Testing Physical and Chemical Properties _____________________________________________ Michigan State University Chemistry Lab 161 Section 27 19 October 2017 Friday 11:00-1:50 Introduction For this experiment‚ there was an unknown white compound that had to be identified. By testing this compound for different properties it made making an educated guess easier to determine
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GRAM STAINING EXPERIMENT CONDUCTED ON 9/29/2013 Introduction: The Gram stain is a useful stain for identifying and classifying bacteria. The Gram stain is a differential stain that allows you to classify bacteria as either gram positive or gram negative. This gram stain technique was discovered by Hans Christina Gram in 1884. The gram stain procedure separates all bacteria into one of two groups - into gram-negative bacteria which do not stain purple and into gram-positive
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1. Obtain 1 gram of an impure‚ unknown substance solid. Make sure to stir the mixture before measuring the sample; record the mixture’s code in the data section. 2. Add approx. 25-50 mL of water and several boiling chips to a 125 mL Erlenmeyer flask and heat the water to a gentle boil using a hotplate. 3. On a second hotplate‚ place a 125 mL Erlenmeyer flask containing the one gram of unknown solid along with a boiling chip. 4. Using a ring clamp‚ slowly pour approx. 5-10 mL of boiling water into
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ways we can differentiate the different species. Looking at these bacterium underneath the microscope is an important step to determine which kind of species it is. All cocci bacteria are not gram positive‚ but this lab will examine only this kind because of their influence in human health. Staphylococcus Aureus is a gram-positive coccus bacterium found in humans. It is frequently found on the mucus membrane in the nasal cavity and on the skin. It also infects many people in the hospital‚ also called
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Identification of Unknown # 15 Abstract. One of the most fundamental differential staining techniques used in the study of bacteriology is gram staining. There are two main types of bacteria‚ gram negative and gram-positive. The purpose of this experiment was to perform a variety of tests to identify the bacteria contained in the unknown sample labeled number 15. The following are the tests that were used to identify the two different bacteria. The
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Gram staining From Wikipedia‚ the free encyclopedia A Gram stain of mixed Staphylococcus aureus (Staphylococcus aureus ATCC 25923‚ Gram-positive cocci‚ in purple) andEscherichia coli (Escherichia coli ATCC 11775‚ Gram-negative bacilli‚ in red)‚ the most common Gram stain reference bacteria Gram staining (or Gram’s method) is a method of differentiating bacterial species into two large groups (Gram-positive and Gram-negative). The name comes from its inventor‚ Hans Christian Gram. Gram staining
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Qualitative Analysis March 20‚ 2015 The purpose of this lab is to identify unknown ions in a solution by using a type of chemical reaction called precipitate reactions. The key to finding which ions are present in a solution is to form a precipitate which makes the ions “come out” in a reaction (McNeil‚ 2013-2014). Water is used in these experiments to act as a solvent. Since water is a polar molecule‚ the slightly positive and negative charges will sometimes pull apart molecules and surround them
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Introduction: The gram stain is one of several laboratory procedures that can be used to narrow down the identities of unknown bacteria. Bacteria have three different shapes; cocci‚ bacilli‚ and spirilla. Since bacteria pretty much have the same reflective index as water‚ a bacteria cell must be dyed so that these shapes can be seen. Materials: Petri dish Dropper Cleansing solution Slides Bibulous paper Inoculation loop Crystal violet dye‚ Iodine Acetone Safranin Water from the sink
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