IDENTIFYING GRAM POSITIVE COCCI As mentioned in Exercise 8‚ “Identifying Gram Negative Rods”‚ identifying bacteria is a common activity in the microbiology lab. Like the game Clue™‚ each time you gather a piece of information to solve the mystery‚ you gather some information that supports some identities and eliminates others from contention. In the lab‚ the process continues as you gather more information until only one microbe remains and all others have been eliminated as possibilities. Thus
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Judson Cotterman III 4/6/2012 Section 185-35L Assignment #3 Gram-positive Pathogenic Cocci Discussion My original hypothesis for the individually collected throat culture on Blood Agar Plates‚ that it would show a mixture of alpha‚ beta‚ and gamma hemolysis‚ was based on the results of the class control samples of S. pneumonia‚ S. aureas and S. epidermis. In these controls S. pneumonia shows alpha and beta hemolysis while S. aureas and S. epidermis showed beta and gamma hemolysis respectively.
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Describe the major differences between gram positive and gram negative bacteria cell walls. The gram negative bacteria cell wall is a thin peptidoglycan layer and an outer cell membrane with a lipopolysaccharide layer. The gram positive bacteria cell wall is a single thick peptidoglycan layer. This wall forms in a mesh like formation of three layers of alternating material. 2. From the procedure that you have carried out‚do you feel that the Gram positive stain is a simple procedure? No‚ because
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9:00Am-11Am Unknown Number 94 Unknown Results Streptococcus salivarius The first step in my unknown identification was to carry out a gram stain to observe the cell shape and arrangement. My unknown turned out to be gram positive bacteria cocci shaped with long chain cell arrangement. The unknown was gram positive because the cells stained blue-violet ‚this indicate that my unknown has ability to retain crystal violet-iodine after alcohol application on the cells which is a gram positive characteristic
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Unknown Microorganisms May 1‚ 2013 This paper is based on an experiment done in the laboratory over a period of time using the standard method of identifying an unknown microorganism relying on features such as morphology‚ arrangements and biochemical reactions. It was done in succession with each step complimenting the other. At the end of the experiment three microorganisms Bacillus azotoformans‚ Staphylococcus aureus‚ and Escherichia coli was identified and one result was inconclusive. The
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difference between a gram positive bacteria and gram negative bacteria is the differences in cell wall composition. Prokaryotes known as eubacteria have three basic forms: rods‚ cocci and spiral. The bacterial cell wall is the single most important contributor to cell shape. In addition to shape of cell wall‚ presence or absence of flagellum‚ and if present‚ positions of flagellum‚ the eubacteria can be classified according to Gram Stain. First and foremost‚ gram positive bacteria are by
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identify a metal‚ each with varying amounts of accuracy. In order to test some of these methods‚ thermal expansion‚ density‚ and specific heat were used to identify two small metal rods. The overall purpose of this experiment was to identify an unknown metal using the three previously stated properties. This means that the percent error should be relatively low‚ meaning that there would be little difference between the values measured in our experiment and the true values of the metal. Density
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Sampling of Gram-Positive Bacteria and Antibiotic Resistance Resting We thought it would be interesting to find out the different bacteria that grow on things we use on a daily basis and the level of antibiotic resistance that those bacteria have. We chose to swab the toilet seat in the MATC women’s bathroom and the ATM machine in the commons area on campus. We were certain the ATM would be dirtier than the toilet seat since the bathrooms get cleaned every day. The first step we took in testing
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first need to know what gram-negative and gram-positive bacteria are and what it’s composed of. Gram-positive bacteria take up the class of bacteria that stain crystal violet in the method of bacterial differentiation. Where gram-negative bacteria are a class of bacteria that does not retain crystal violet. Instead they are counter-stained pink by Safranin‚ and because of this‚ identifying positive is possible. One of the many differences between gram-negative and gram-positive bacteria is their peptidoglycan
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Objective: To successfully separate and identify all three unknown cations‚ found in a mixture and belonging to one of two specific groups (2 from one and 1 from the other). In order to do this we will be using various tests and reactions and observing the effect they had on our solution. Materials: Pasteur pipets Bunsen Burners Stirring rods Excess reagents Hot plates Sample QA unknown 101-5.7 Table of steps and observations: Step/Test |Observation |Inference | |2-1
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