Beta-Galactosidase and Western Blot 0 1. EMG 9 and EMG 26 contain strain _lac-_(I- Z+ Y+) and strain _lac -_ (I+ Z- Y-)respectively.Three genes huddled together on the chromosome are required for two strains of _E.coli_ to utilize lactose.Consisting of three genes‚ namely‚ _lacZ_‚ _lacY_ and _lacA_‚ the _lac_ operon orderly handles these genes to code specific enzymes necessary for the metabolism of lactose. The genes _lacZ_‚ _lacY_ and _lacI_ would code for beta-galactosidase‚ galactosidase permease
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Paul Childrey Professor Lisa Lykins English 111 September 26‚ 2012 “How Do You Kill 11 Million People?” How do you kill eleven million people and get away with it? It is a question that certainly needs to be thought about and answered. My favorite period in history is the World War II era. My thinking was really changed when I read the book “How Do You Kill 11 Million People?” As the author stated so vividly‚ “you begin with a lie” (Andrews). This book began by questioning the history that
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How do you spend your free time? There are many different things which you can do in your free time. Personally I don’t have much free time. I am preparing to my mature exams. However‚ there always happen some free moments on the weekends. I don’t do any especially exciting things then. Sometimes I simply lie on the sofa and watch TV. I like series movies‚ like "Friends". I must admit that I also watch soap operas to entertain. In my mind you cannot learn anything watching TV so I generally choose
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1. How many points have you earned this semester? What was your class average? During this semester‚ I have earned 545 points. My class average is 96%. 2. How would you judge your “attendance” in this class? (how often did you log into Blackboard and/or check your email)? In this class‚ I believe my attendance was very excellent. Over the course of this semester I logged into Blackboard almost every day to check the announcements and assignments listings for this class. As well as my email‚ I check
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How do I define Success? Success could be seen in someone who is happy and feels fulfilled in what they are doing in life…no matter what kind of job they have. I mean look at Dr. Pol‚ he literally has to go the extremes to help cure an animal’s sickness but he looks like the happiest man on earth. I determine myself‚ Amanda Mortilla‚ as someone who is not successful yet. I think of it like that because I am just starting my path to get find my dream career so I am happy in the future. I am happy
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Victoria Jeudy English Comp. 2 Professor Shirokova March 26‚ 2013 How do you say it? Alberto Alvaro Rios writing is very compelling. He makes statements that give you a sense of wonder. Meaning that he makes certain statements that make you think about what he’s talking about‚ because his statements are so powerful and he has a sense that tends to make you respect what he his saying. Seeing how I am bilingual‚ I have a sense of understanding of where Rios is coming from when he tells his
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problem we are studying was to determine the cellular damage of various concentrations of propanol‚ ethanol‚ methanol‚ NaCL‚ and sodium dodecyl sulfate henceforth known as SDS on Beta vulgaris to ultimately distinguish the ability of each solution to permeate the membrane of the Beta vulgaris. • Beta vulgaris contains a beta cyanin which is a water-soluble red-pigment‚ which remains in a healthy vacuole surrounded by the tonoplast membrane. When we damage the tonoplast membrane and thus disrupt the
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How do you respond to Gerald in An Inspector Calls? How does Priestley make you respond as you do by the way he writes? (30 Marks) The character of Gerald in An Inspector Calls is one that produces different responses; this is due to Priestley’s writing style. During the start of the play Gerald takes a low level status but by the end of the Play he is defiantly trying to save himself and the family. How does Priestley make us feel like that? Well it’s all down to the stage directions and how the
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Riddle : How do you eat an elephant? Solution : In man-size bites! This riddle serves to remind ourselves that being overly ambitious or unrealistic‚ to go for too much‚ more than can be handled at any one go‚ is not going to get one very far! Every vision is the result of an extended period of hard work‚ of a series of tasks or objectives‚ stacked up in a structured way. Realising the vision requires devotion to and successful execution of each and every one of these tasks. But first‚ how this
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sufficient. Sometimes my dad will give me extra money to go shopping. when I go shopping I buy lots of clothes from topshop and some new shoes‚ but it is nearly Christmas so I am saving up for Christmas presents for my family. 2. no‚ i do not have a little job because i do not have time to work and i am too young. but if i could‚ i would like to have a little job on Saturday evenings‚ in a restaurant being a waitress. Then i could earn my own pocket money. 3. (yes‚ last summer I had a job as a waitress
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