Gram Staining Introduction Prokaryotes are a large group of organisms with no membrane bound organelles. They consist of two domains: Archaea and Bacteria. These organisms are only found in extreme environments such as volcanoes. Prokaryotes are still being researched and are a very diverse group. In this lab we focused on trying to identify if the bacteria found had a lot of peptidoglycan by gram staining. Testing this could be done by using a Petri dish full of agar and testing different bacteria
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The Gram staining method is named after the Danish bacteriologist who originally developed it in 1882‚ Hans Christian Gram. The Gram staining process is one of the most important staining techniques in microbiology. It is almost always the first test performed for the identification of bacteria. The primary stain of the Gram’s method is either crystal violet or methylene blue‚ each almost equally effective. The microorganisms that retain the crystal violet and iodine mixture appear purplish brown
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Introduction: The gram stain is one of several laboratory procedures that can be used to narrow down the identities of unknown bacteria. Bacteria have three different shapes; cocci‚ bacilli‚ and spirilla. Since bacteria pretty much have the same reflective index as water‚ a bacteria cell must be dyed so that these shapes can be seen. Materials: Petri dish Dropper Cleansing solution Slides Bibulous paper Inoculation loop Crystal violet dye‚ Iodine Acetone Safranin Water from the sink
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NAME: PRAJWAL BHARADWAJ Ampicillin‚ Vancomycin‚ and Tetracycline Effectiveness on Gram Positive and Gram Negative Bacteria. INTRODUCTION: Bacteria are microbial organisms which are present in various environments. Many bacteria are good and help humans synthesize materials and assist in biological processes‚ such as digestion. However‚ some bacteria can cause harmful diseases. When harmful bacteria infects the body‚ people take antibiotics in order to suppress bacteria. Different antibiotics have
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Gram Staining Gram Staining is a method of differentiating bacterial species into two large groups (Gram-positive and Gram-negative). Gram Staining is a way to separate one large group of bacteria into two. Crystal violet is used to dye the cells‚ which is the primary stain. Those that retain the color are grouped as Gram-positive‚ and those that do not retain the color are grouped as Gram-negative. Many of the Gram-negative bacteria are pathogenic‚ making this process useful for detecting infections
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Diffusion In Agar Cubes Is Bigger Better? OR Is Smaller Smarter? Adapted from Flinn Scientific Publication No. 10110 Introduction: Diffusion is one of the very important processes by which substances such as nutrients‚ water‚ oxygen‚ and cellular wasted are transported between living cells and their environment. This activity will help you explore the relationship between diffusion and cell size by experimenting with model “cells.” In this experiment‚ you will use agar cubes to which
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differed between my group’s nutrient agar and MAC agar plates. In courgette‚ there was moderate growth in nutrient agar while it was heavy in Mac agar. In beef broth‚ there was slight growth in nutrient agar while it was negative in MAC agar. In fresh basil‚ growth was heavy in nutrient agar while it was heavy in MAC agar. Where cottage cheese was the ingredient‚ both plates had heavy growth. For walnut‚ growth was slight in nutrient agar while it was negative in MAC agar. Generally‚ the slight difference
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Laboratory(TFMB1001) Report Experiment Tittle Gram stain Experiment Date 24October2014 Submission Date 31October2014 Students Name Tittle Gram stain. Objectives Aim of this experiment is to differentiate between the two major categories of bacteria ‚ gram positive and gram negative. Through this experiment‚ gram staining skills develop. More understanding the types and morphology of bacteria. Expected experimental result‚ Escherichia coli (E.coli ) is a negative gram bacteria which stain pink colour
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Gram Staining of Bacteria Since bacteria is really small microbiologist use stains to help them see bacteria more clearly under the microscope. Many of the stains they use color the bacteria cells directly and are called direct stains. Bacteria cells have a slightly negative charge while direct stains have a slightly positive charge that helps the stain bin to the bacteria. The strength of the binding from the stains depends on the make-up of the cell wall itself. Gram staining is the most helpful
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GRAM STAIN THEORY: The most widely used staining procedure in microbiology is the Gram stain‚ discovered by the Danish scientist and physician Hans Christian Joachim Gram in 1884. Gram staining is a differential staining technique that differentiates bacteria into two groups: gram-positives and gram-negatives. The procedure is based on the ability of microorganisms to retain color of the stains used during the gram
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