The identification of an unknown bacterial environmental isolate through a series of morphological‚ physiological and differential exercises. The purpose of the environmental isolate report is to learn what is necessary in order to take an unknown environmental isolate (EI) and identify it. This was achieved through a series of exercises that provided information on the morphological‚ physiological and biochemical traits of the EI which were then compiled and interpreted in order to make a presumptive
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average‚ there are about 125 million rod cells in the human retina. Rod cells are almost entirely responsible for night vision. Rod cells do not process fine details or color differences‚ but are highly sensitive to light. A single flash of light is all that is require to produce a response from a rod cell. Rod cells function as specialized neurons that convert visual stimuli in the form of photons (particles of light) into chemical and electrical stimuli. Rod cells are responsible for perceiving
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Margaret E Gibson July 20‚ 2009 Microbiology Dr. Metera Lab Report 3: Labs 7 and 8- Metabolism and Biochemical Tests Abstract This experiment focused on metabolism and biochemical tests. The goal of performing these tests was to differentiate microbes from one another and to compare how metabolic and biochemical processes differ from species to species. The tests performed include: the Fermentation of Sugars Test (sucrose‚ glucose‚ and lactose)‚ the Urease Test‚ the Fermentation
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differences of each is vital to treat a patient correctly. The purpose of this report is to explain the process and steps used to identify a certain microorganism referred to as the unknown. Materials and Methods A test tube with an unknown microorganism will be retrieved. Once the test tube is retrieved‚ a steak for isolation will be completed in order to produce isolated colonies of the organism on an agar plate. The unknown test tube with the bacteria is flame sterilized using a Bunsen burner. Once
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Macromolecules Lab Purpose: to determine a method of testing for macromolecules. Materials: Knowns: Unknown: Test Solutions: Glucose solution Unknown solution Iodine solution Gelatin solution Benedict’s solution Starch solution Biuret solution Oil Brown paper Water Procedure: 1. Create a data table. 2. Label 5 test tubes with known solutions. 3. Add 10-20 drops of each known solution to respective test tubes‚ do not mix pipets! 4. Add 3-5 drops of
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Name SOLUBILITY CURVES Answer the following questions based on the solubility curve below. Which salt is least soluble in water .. at 2O° C? 2. How many grams of potassium chloride can be dissolved in 200 g of water at 80° C? IO 3. At 40° C‚ how much potassium _ __nitrate coin be dissoiu$tl ^n 30D.g of water? ------W- ’1 80 70 ...- O --60 0 5© 40 4. Which salt shows the least change 30 In solubility from 0° - 100° C? 20 10 At 30° C‚ 90 g of sodium
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Discussion The possible identity of the unknown organisms in the mixed culture was limited to bacteria that we had worked with previously in lab. Initially a Gram stain was conducted in order to distinguish the unknown bacterium as a Gram-positive and/or a Gram-negative organism (Lancaster and Bennett‚ 2012; Kellenberger‚ 2001). Based upon the results‚ both Gram-negative and Gram-positive bacteria were observed in the unknown mixed culture (Table 1 and Table 2; Kellenberger‚ 2001). In order
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Introduction: The gram stain is one of several laboratory procedures that can be used to narrow down the identities of unknown bacteria. Bacteria have three different shapes; cocci‚ bacilli‚ and spirilla. Since bacteria pretty much have the same reflective index as water‚ a bacteria cell must be dyed so that these shapes can be seen. Materials: Petri dish Dropper Cleansing solution Slides Bibulous paper Inoculation loop Crystal violet dye‚ Iodine Acetone Safranin Water from the sink
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by aseptically inoculating a tube of phenol red sucrose broth‚ and a tube of phenol red arabinose broth with the unknown culture and incubating at 37 degrees Celsius for 48 hours. After incubation‚ the two tubes were examined for color change. The eighth test was to find out if the bacteria in question had flagella. The motility test was performed by aseptically inoculating the unknown bacteria into a tube of TSA broth and allowing it to incubate for 24 hours at 37 degrees Celsius. After the TSA
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identification of two unknown bacteria. An unknown labeled as number 3 was given out by the lab instructor. The methods that have been lectured and absorbed pertaining to identifying
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