we first need to know what gram-negative and gram-positive bacteria are and what it’s composed of. Gram-positive bacteria take up the class of bacteria that stain crystal violet in the method of bacterial differentiation. Where gram-negative bacteria are a class of bacteria that does not retain crystal violet. Instead they are counter-stained pink by Safranin‚ and because of this‚ identifying positive is possible. One of the many differences between gram-negative and gram-positive bacteria is their
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Name: Danielle Title: Unknown Lab Report Introduction: There are many reasons for identifying an unknown bacterium. The reasons range from medical purposes‚ such as determining if the unknown could cause ailments in living things or knowing what microorganisms are needed to make antibiotics. The experiment was done by applying methods in order to identify an unknown bacterium. An unknown bacterium was handed out by Dr. Honer. The appropriate tests were prepared and applied. The
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Unknown Lab Report April 25th‚ 2006 Introduction The purpose of this lab was to identify two unknown bacteria cultures using various differential tests. The identification of these unknown cultures was accomplished by separating and differentiating possible bacteria based on specific biochemical characteristics. Whether the tests performed identified specific enzymatic reactions or metabolic pathways‚ each was used in a way to help recognize those specifics and identify the unknown cultures
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Introduction Gram staining was developed by Christian Gram in the 1800’s‚ a Danish bacteriologist. (Smith and Hussey‚ 2005) It was the first differential staining technique and most common used in microbiology. Furthermore‚ bacteria are transparent and cannot be seen through the microscope. For that reason‚ Gram staining is an important tool for distinguishing between two main types of bacteria Gram-positive and Gram-negative. The Gram stain differentiates the Gram positive and gram-negative on the basis
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Determining the Unknown Microorganism from a Mixed Culture Mylah Ferland‚ Rivier University MICRO 206‚ March 16th‚ 2016 Introduction: An unknown bacterium experiment‚ this experiment was done to discover what bacterium was in a mixed culture sample. This experiment worked with enteric bacteria which are members of the Enterobacteriaceae and live in the intestinal tract (Willey et al.‚ 2014). Also found in Willey et al (2014) is some characteristics of enterics such as they all degrade sugars by
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Purpose: To identify an unknown bacterial specimen using basic laboratory technique and biochemical tests. The unknown bacteria will be one of the following: Enterococcus faecalis‚ Staphylococcus saprophyticus‚ Escherichia coli‚ Enterobacter aerogenes‚ Proteus vulgaris‚ Salmonella [I assume typhimurium]‚ or Shigella [either flexneri or sonnei‚ we used both in our lab during the semester]. Procedure {and observations}: Observe bacterial colony morphology. {Colonies are large‚ beige or
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Unknown Lab Report Unknown Organism #6 Ann Le (Phuoc) May 6‚ 2010 Dr. Carrington Microbiology Lab- MW 12:50 Le 1 I. Introduction My unknown organism #6 is Morganella morganii‚ which is a gram-negative bacillus rods commonly found in the environment and also in the intestinal tracts of humans‚ mammals‚ and reptiles as a normal flora. (3‚ 5) This bacterium Morganella morganii‚ was first discovered in the 1906 by a British bacteriologist named H. de R. Morgan. (2) Despite its wide
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In the unknown identification labs‚ we have identified our unknown as Pseudomonas aeruginosa. Pseudomonas aeruginosa is Gram negative and rod shaped that we found to be motile in the lab. Our strain of P. aeruginosa formed colonies that were round in shape and had scalloped margins on nutrient agar. On our agar slant‚ the P. aeruginosa colonies had a filiform appearance on the edges. I think we correctly identified our unknown as P. aeruginosa because we performed several different tests‚ eleven
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I immediately made a streak plate and started the Gram Stain protocol1. In the first two slides‚ I made and stained‚ my results were ambiguous. Without putting the slide into the microscope‚ I noticed one was pink and the other purple. To resolve it‚ I discarded my slides and started again and was extremely careful in proceeding the Gram Stain. I concluded that my unknown A was gram-negative bacilli. Using the dichotomous key for gram-negative bacilli I started the protocol for the lactose test2
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Requirements for Unknown Report You have been provided a mixed culture (a gram positive and a gram negative) from which you should have isolated each type of organism‚ described their colonies on a TSA plate‚ and described their cellular morphology. You were graded on this activity separately. After you have isolated your gram negative in pure culture you will be subjecting it to various biochemical tests and making observations about the cells‚ colonies‚ growth temperature preferences‚ etc. in
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