with distilled water by centrifugation until the yellow colour disappears. Avoid excessive washing‚ as this may weaken the impregnation. After the last washing‚ discard supernatant‚ leaving about 0.1 ml of the sample. Add 0.01 ml of Mayer’s albumen glycerol solution and mix gently to avoid foaming. Incubate the cells in this solution for about 30 min. Transfer the precipitate with ciliates evenly on 2 or 3 grease-free cover glasses (24×24 mm) and distribute across the surface using a mounting eyelash
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neatness of the print and general acceptability. The final sample made use of two coatings of the ink mixture using carbon black‚ with coupon bond as the medium. The pigment (carbon black) to vehicle (glycerol) ratio of 1 g : 6 mL and 1 g : 7 mL was used using carbon black as pigment with glycerol as vehicle. Although the commercial bond paper has better quality‚ since it uses more technologically advanced methods‚ the use of the experimental carbon paper was acceptable. INTRODUCTION Carbon paper
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Concept 6: Nucleic Acids Practice (1 page) Review (10 pages) Self-Quiz Self-Quiz 1. The monomers used to synthesize proteins are called: a. nucleotides b. amino acids c. fatty acids d. sugars e. glycerol 2. A nucleoside contains: a. a pentose sugar b. a purine or pyrimidine base c. one or more phosphate group d. all of these e. a and b‚ but not c 3. The molecule shown below is: a. a purine base
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list | 5 minutes | Closing | Sign-ups for email | Materials Per batch of bio plastic (Ideally per person): * 100g of potato * 300cm3 of distilled water * 25ml of water * 3cm3 of hydrochloric acid or vinegar * 2ml pure glycerol * 3cm3 sodium hydroxide * Food colouring Tools * Grater * Pestle & Mortar * Strainer * Beaker/Jars * Measuring cylinder/jug * Weighing scales * Indicator Paper * Portable hob Preparation * Prepare
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1. | The stability of the three-dimensional shape of many large molecules is dependent on | | A. | covalent bonds. | B. | ionic bonds. | C. | hydrogen bonds. | D. | van der Waals attractions. | E. | hydrophobic interactions. | | See Concept 2.2: Atoms Interact and Form Molecules | Points Earned: | 0/1 | Correct Answer: | C | Your Response: | A | 2. | Which of the following statements about water is true? | | A. | Water has a low heat of vaporization. | B. | Water has
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in water and are soluble in so-called fat solvents (diethyl ether‚ acetone‚ hexane and carbon tetrachloride). The elements present are carbon‚ hydrogen‚ and oxygen which are combined as esters of various fatty acids with the tri-hydroxy alcohol‚ glycerol. There are many different categories of lipids like triacyglycerols‚ phospholipids and steroids and each category has different components present in its structure. In this experiment‚ fatty acids are prioritized. Fatty acids are components of many
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In biology‚ there are four major biological macromolecules that are found in the cell‚ carbohydrates‚ lipids‚ nucleic acid‚ and proteins. Biological macromolecules are also known as organic molecules. Biological macromolecules are very large molecules that are of a key importance to living organisms. Most biological macromolecules are built by joining smaller molecule subunits. Carbohydrates are large groups of organic compounds found in foods such as sugars‚ starches‚ glucose‚ fructose‚ galactose
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error in the composition of the LA agar media ingredients. However‚ when SWC agar media used‚ there was good growth of bacteria and bright production of light. When comparing both media‚ the SWC media contains 10ml of Glycerol whereas there was no Glycerol added in the LA media. Glycerol promotes good luminescent production in the bacteria. Moreover‚ the amount of Bacteriological agar as solidifying agent was increased in the SWC media
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Properties of Alcohols and Phenols Experiment #3 Objectives: To observe the solubility of alcohols relative to their chemical structure‚ to perform chemical tests to distinguish primary‚ secondary and tertiary alcohols‚ and a color test for phenol. Introduction The general formula of an alcohol is ROH in which the R is an aliphatic hydrocarbon group. Alcohols may be looked upon as derivatives of water‚ HOH. One hydrogen of water is substituted by an alkyl group‚ R. Like water‚ alcohols show hydrogen
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ml 4x Upper gel buffer 0.5 M Tris-Cl‚ pH 6.8‚ 0.4% SDS 2 121.1 70-80 80 ml 4.1 10% SDS 1L: 100g SDS into 1 L‚ heat to 68oC for solubility. pH ~6.6. 5. 5X SDS Loading Sample Buffer 100 ml Stock solution 250 mM TrisHCl pH6.8 2M 10% SDS 30% Glycerol 5% β-mercapitalethanol (or 0.5M DTT) 0.02% bromophenol blue 0.04% Add volume 12.5 ml 10 g 30 ml 5 ml 52 ml 6. 6X DNA loading sample buffer: (40% sucrose‚ 0.01-0.02% BPB) 100 ml Add 40 g sucrose to 50 ml 0.04% BPB solution‚ adjust final volume 100
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