unknown compound. In this experiment‚ chromatography and titration were performed to identify the unknown amino acid. Within experimental error‚ the results were consistent with the reference literature cited in this report. Experimental Thin Layer Chromatography The amino acid standards used in this experiment were Alanine‚ Glycine‚ Serine‚ and Histidine. These standards and the unknown were separated by performing a method of chromatography. Thin layer chromatography (TLC) was performed by using a mobile
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Objectives: To use the process of chromatography to separate plant pigments. To compare the plant pigments found in spinach and red leaf lettuce leaves. Hypotheses: I believe the spinach leaf in the acetone will have a higher pigment of chlorophyll a and the red leaf lettuce will have a higher pigment of anthocyanin. I believe the spinach leaf in distilled water will have a higher pigment of chlorophyll a and the red leaf lettuce will have a higher pigment of anthocyanin. Discussion Questions:
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Chromatography of Food Dye Chem 180-DL3 Abstract: The goal of this experiment was to use different dyes to determine the Rf value. Chromatography is done to determine the presence of a mixture in a substance. In this experiment different dyes were used such as McCormick food coloring‚ M&M’s‚ Kool-Aid‚ and the dyes that came with the lab kit. Experiment and Observation: In this experiment a 50mL beaker was filled with luke warm water and a pinch of salt water was dissolved in it
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Linearity: A linear correlation coefficient factor was obtained between the peak area used and the absorbance Verses concentrations of lamivudine‚ zidovudine and nevirapine. The calibration curves were linear for concentrations between 15-150 µg/ml. The linearity of the calibration curves was validated by the values of the correlation coefficients (r2). The correlation coefficients were 0.999 for lamivudine‚ 0.999 zidovudine and 0.999 for nevirapine. The results of the linearity experiment are listed
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Ink Chromatography Lab Background Information Paper chromatography is an analytical method that is used for separating and identifying mixtures of substances into their smaller parts. Paper chromatography works because the ink used contains several dyes (pigments) that when place on porous paper are dissolved in a solvent by capillary action. When the pigments (solutes) are dissolved in the solvent (water & alcohol mixture) they move through the paper at different rates depending on their
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able to experimentally analyze and isolate amino acids by separation techniques. One such method of separation is thin layer chromatography (TLC). In this method‚ a silica coated aluminum plate is spotted with the solution being separated‚ and the plate is placed in a developing jar containing a small amount of solvent. With time‚ the solvent
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Abstract Finding the melting point of an organic substance is a practical and efficient way for scientists to identify an unknown substance or determine a known substance’s level of purity. When organic substances are mixed together in varying degrees they take on a melting characteristic that is lower and broader than in its pure form. This property was manipulated in the lab to observe the various melting points of Naphthalene and Biphenyl when the percentage of composition was altered. A
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Serratiopeptidase drug was purchased from MARS Therapeutics & Chemicals limited‚ Hyderabad‚ India. The standard drug Fenofibrate was purchased from USV limited‚ Himachal Pradesh‚ India. Assay kits for serum Total Cholesterol (TC)‚ Triglycerides (TGL)‚ High density lipoproteins were purchased from Erba Mannheim‚ Transasia Bio-Medicals limited‚ Himachal Pradesh‚ India. All other chemicals were of analytical grade. The experiment was conducted using [12] male Albino Wistar rats (150-200g)‚ at about
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Institute of Technology MSc in Oil & Gas Technology “CONTRACT LAW” “THE INTERPRETATION OF LEGAL TERMS ABOUT CONTRACTUAL OBLIGATIONS OF THE PARTIES IN AN OIL OR GAS EXPLORATION/EXTRACTION CONTRACT” Authors: Andreou Christos Georgiou Manolis Kakanis Iordanis Skarvelas Stathis Supervisor: Pr. K. Kalambouka November 2012 ABSTRACT The purpose of this assignment is to identify the contractual obligations of the parties in oil and gas exploration and extraction contracts. The
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Candy chromatography My experiment is called candy chromatography. This project is mainly about the colors that are mixed with other colors to make candy markers Koolaid and much more. I think that only the color of the candy is going to show up on the coffee paper. I think that threw the entire experiment the color in the candy is going to show up the entire time. I think this is going to happen because most of the colors are solid colors. The material and equipment used was a sample of candy
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