References: 1) E. Stahl‚ Thin Layer Chromatography‚ Springer-Verlag‚ New York‚ 1969 2) Camag Scientific‚ Inc.‚ 515 Cornelius Harnett Drive‚ Wilmington‚ NC 28401‚ phone 800-3343909‚ fax 919-343-1834 3) Desaga GmbH‚ P.O. Box 101969‚ D-69009 Heidelberg‚ Germany‚ phone 0-62-21-83590‚ fax 0-62-21-840887
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Resolution of Matter into Pure Substances – Paper Chromatography Abstract The purpose of this laboratory was to determine how different substances have different solubilities in a given solvent. This was accomplished using the method of chromatography. The solubility of each substance was first determined by dropping a drop or two of each on a line drawn 2 inches above the bottom of a piece of paper. Then the paper was lightly bent in order to tape both sides of the paper leaving about a 4 centimeter
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The reason for conducting the research is to know how the colors of our favorite candy-coated sugary rewards came to be and that method of knowing is what we call “Candy Crhomatography”. Chromatography by definition according to Webster’s dictionary means “ a method for separating the constituents of a solution (gas or liquid) by exploiting the different bonding properties of different molecules”. In this case‚ the molecules being broken down are the different molecules that make up a certain color
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Gas analysis techniques Terms for gas analysis techniques Concentration The term concentration describes the amount of a substance‚ expressed as mass‚ volume‚ or number of particles in a unit volume of a solid‚ liquid‚ or gaseous substance e.g. alcohol in beer or oxygen in air. Different units are in use to describe concentration in gases: Mass concentration Concentration expressed in terms of mass of substance per unit volume [g substance/m3 gas volume] ¢ Volume concentration Concentration
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Review the behavior of the gas phase vs. the liquid phase. Solution: a) The volume of the liquid remains constant‚ but the volume of the gas increases to the volume of the larger container. b) The volume of the container holding the gas sample increases when heated‚ but the volume of the container holding the liquid sample remains essentially constant when heated. c) The volume of the liquid remains essentially constant‚ but the volume of the gas is reduced. The particles in a gas are further apart than
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Full Report on Exercise 4.2 ESTIMATION OF PROTEIN CONCENTRATION BY SPECTROPHOTOMETRY And Exercise 4.3 GEL FILTRATION CHROMATOGRAPHY Joel Don M. Untalan CHEM 160.1 – 1L AY 2013 – 2014 Groupmates: Sonette Yao Kristopher Quilan Laboratory Instructor Carmelo C. Briones I. Introduction Analyzing proteins in determination of protein concentration by spectrophotometry is important. It determines to what concentration of a certain protein is in a crude sample. In this technique‚ a wide
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Gas Laws Name Institution The 3 Gas Laws Introduction The three gas laws include: Gay-Lussac’s law‚ Boyle’s law and Charles’ law. When combined with Avogadro’s law the three laws can be generalized by the ideal gas law. Gases possess observable properties which include‚ mass‚ pressure (P)‚ thermodynamic temperature (T) and volume (V). These properties are related to each other and the state of a gas is determined by their values. The three laws are derived from these properties. Discussion
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Food Analysis * Concerned with development of criteria of quality and identity… * Process in w/c sample is treated in a series of steps Importance: 1. It’s necessary in the dev’t and enforcement in standard of identity‚ purity and feel of processed food products. 2. It can provide studies design to improve and control the quality of natural processed food. 3. Can help in the determination of the composition of products in their normal and abnormal storage conditions. Methods
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GAS LAWS Properties of Gases Gases expand into any available volume • gas molecules escape from open containers. Gases are completely miscible • once mixed they will not spontaneously separate. Gases are described in terms of T‚ P‚ V and n Volume‚ Amount and Temperature • A gas expands uniformly to fill the container in which it is placed – The volume of the container is the volume of the gas – Volume may be in liters‚ mL‚ or cm3 • The temperature of a gas must be indicated
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Date:7.6.2013 Objectives: Part 1: (i)To learn the technique of TLC and the visualization of colourless components. (ii)Identify the unknown drug by a TCL comparison with standard components. Part2 :to learn the separation technique by using TLC plate in separating a mixture of compound by using spinach leaf. Apparatus:UV lamp‚capillary ‚250 ML beaker Materials:aspirin‚acetaminophen‚caffeine‚unknown A‚unknown B‚TLC plates‚ethyl acetate‚Hexane‚acetic acid‚iodine Introduction: Procedure:
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