Micro-extraction of urine and/or stomach contents and analysis by TLC Abstract Thin Layer Chromatography (TLC) is one of the oldest chromatographic techniques used for the identification of compounds and for determination of the presence of trace impurities. The aim of the experiment was to determine two unknown samples (X and Y) by comparing results to the known standard drugs‚ of which there was five‚ using a specific TLC method. The same technique was used for the determination of
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Chromatography – Analyzing Analgesics by TLC and Isolation of β-Carotene by Column Chromatography Introduction/Background: Flavonoids are an important group of additives that can be defined as pure substances either natural‚ extracted from raw materials or synthetic. Chromatography is the separation of two or more compounds or ions caused by their molecular interactions with two phases – one moving and one stationary (Weldegirma 2012). Three types of chromatography are used
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Thin-layer Chromatogragaphy : Analysis of Drug Components CHEM221 Date experiment performed : January 20‚ 2014Date Submitted : January 27‚ 2014 Introduction This laboratory has its first objective to familiarize with the thin layer chromatography (TLC) technique. Second‚ each student has to identify the unknown components by comparing it to one of the tree dissolved liquid analgesics: Acetaminophen‚ Aspirin and Caffeine. Finally‚ calculating the Rf value by measuring the mobile and stationary phase
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The progress of the reaction was monitored in my case using two TLC plate. It first started off with the spotting of Standard benzoin and benzil which were provided in the lab and followed by the addition of the reaction mixture at once it starts changing colour/boiling‚ then at 10 and 20 mins into the reflux. Once all the necessary steps were spotted‚ the TLC plate was placed in in a beaker containing CH₂Cl₂(methylene chloride)‚ which was used as the developing solvent in this experiment. To check
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Experiment 2: IDENTIFICATION OF UNKNOWNS BY TLC AND MP IN COMBINATION Introduction: Thin layer chromatography (TLC) is one of the most valuable techniques in organic chemistry. This is a best method of separating and identifying mixtures of two or more compounds. The separation is accomplished by the distribution of the mixture between two phases: one that is stationary and one that is moving or mobile. Chromatography works on the principle that different compounds will have different
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eluate were collected‚ this process is the column chromatography (CC). The purity of the components was determined by using the thin layer chromatography (TLC). Ultraviolet spectroscopy and iodine staining were used to visualize the developed TLC plate and the Retention Factor was measured. Keywords: Chromatography‚ column chromatography‚ TLC and visualizing agent‚ elution‚ capsicum frutescens Introduction Chromatography can be defined as the separation of a mixture‚ in which the mixture is
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Introduction to Business Law- Assignment 1B Advise TLC Ltd. as to any possible action they may have for misrepresentation against answer Ltd. _____________________________________________________________________ Treitel in The Law of Contract (2003) defines a contract as: “An agreement giving rise to obligations which are enforced or recognised by law. The factor which distinguishes contractual from other legal obligations is that they are based on the agreement of the contracting
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Title: Thin-Layer Chromatography Objectives: Part1: 1. To learn the technique of TLC and the visualization of colourless components. 2. To identify an unknown drug by a TLC comparison with standard compounds. Part 2: To learn the separation technique by using Thin Layer Chromatography plate in separating a mixture of compounds into individual pure compound by using Spinach Leaf. Introduction: (i) General Concepts Chromatography is a common and powerful method used to separate and analyze complex
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Making TLC Plates from Bulk TLC Silica Gels Many TLC users prefer to use pre-coated TLC plates‚ but others because of their special needs‚ or because they need special additives or a special thickness‚ coat their own TLC plates. This paper is a quick review of what is needed for the process. Initial instructions for plate coating: If reproducible plates are needed‚ then the user should invest in a coating device from Camag or Desaga (addresses below). The devices consist of a tray that holds
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Abstract: Saponification is an esterification process that is used to make so from the hydrolysis of fats and oils. This process have the glycerol molecule‚ and a sodium carboxylate salt in most case sodium stearate being formed‚ however this salt depends on the base being used. In the experiment 23ml of vegetable oil was mixed with 20ml of ethyl alcohol along with 20 ml of 25% sodium hydroxide in a 250-mL Erlenmeyer flask. The content was then heated on a water bath whilst gently mixing with
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