Abstract: In this Lab we used the chemical DPIP to detect the rate of succinate broken down by the mitochondrial solution. We detected the amount of DPIP in the solution with a spectrophotometer and measuring the absorbance of light at the 600nm range. DPIP is a useful chemical to use in this experiment because it goes from a blue color when oxidized to a colorless liquid (Ogura‚ 281)‚ this is due to the hydrogen ions and electrons released during the transitional step between succinate and fumarate
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Introduction In the amylase lab‚ it was being tested if amylase‚ an enzyme found in saliva‚ would be denatured by being put in an acid or high temperatures. This lab is about denaturing amylase. It is tested by exposing it to pH and temperature changes. It is then mixed with Benedict’s solution‚ is a solution that changes color from blue to reddish brown when maltose is present. Amylase breaks starch into maltose‚ so is the amylase isn’t denatured‚ it should change colors. Amylase is an enzyme
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Osmosis The purpose of this lab is to study how membranes of plant and animal cells react when exposed to different solutions. The first experiment involves purple onion skin and the second involves rat blood in various solutions. I needed to understand certain terms before preforming this lab to be able to efficiently explain what is happening to the cells. Diffusion is the tendency of a substance to move down its concentration gradient from a more concentrated to a less concentrated area.
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concentrations of sucrose solutions. Sucrose solution (M) Weight Before (g) ± 0.01 (A) Weight After (g) ± 0.01 (B) 0 4.56 4.31 0.2 4.22 4.28 0.4 4.13 4.08 0.6 4.57 4.45 0.8 4.60 4.39 1.0 4.70 4.52 Distilled water is represented by 0M. Table 2: Amount of sucrose solution (ml) before and after the experiment. Sucrose concentration (M) Before experiment (ml) ± 0.01 (A) After experiment (ml) ± 0.01 (B) 0 150 142 0.2 150 142 0.4 150 143
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Lab Report Procedure 6.1: Perform Benedict’s test for reducing sugars. Introduction: Benedict’s tests allows for the detection of the presence of reducing sugars. All monosaccharides are reducing sugars since all of them have active carbonyl group. Some disaccharides that are exposed to a carbonyl group are also reducing sugars but less reactive than monosaccharides. By mixing the sugar solution with Benedict’s solution and heating them‚ a redox reaction will occur. The copper (II) sulphate
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Bacterial Transformation Lab Report Backround: The plasmid pGLO contains an antibiotic-resistance gene‚ ampR‚ and the GFP gene is regulated by the control region of the ara operon. Ampicillin is an antibiotic that kills E. coli‚ so if E. coli‚ so if E. coli cells contain the ampicillin-resistance gene‚ the cells can survive exposure to ampicillin since the ampicillin-resistance gene encodes an enzyme that inactivates the antibiotic. Thus‚ transformed E. coli cells containing ampicillin-resistance
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which light color would cause the spinach sample to photosynthesize the most within the given time. In order to understand photosynthesis‚ we need to know that chloroplasts absorb light energy from the sun‚ the plant then takes that energy along with water and carbon dioxide and changes it to sugar and oxygen. This happens in order for the plants to grow. But‚ light energy comes in a spectrum of colors called visible light. When visible light is absorbed it is also reflected‚ such as the green and yellow
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Lab 2 Part 1: 10 points Learning Objectives and Outcomes Analyze a mixed-version Microsoft Windows environment and identify the issues described in Microsoft Security Advisories. Assignment Requirements You are reviewing the security status for a small Microsoft workgroup LAN. The workgroup contains many distinct separations in the network as determined by group memberships. The network consists of computers working on a variety of client platforms‚ such as Windows XP‚ Windows Vista‚ and Windows
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Chemistry lab report Experiment 32 Voltaic Cell Measurements Dinmukhamed Yeraly Partner Azamat Bashabayev General Chemistry II lab Instructor Rostislav Bukasov Nazarbayev University Introduction Most of the chemical reactions can be classified as redox reactions‚ which include two half reactions‚ oxidation and reduction respectively. To measure the tendency for a readox reaction to occur‚ special apparatus called voltaic cell can be used. It is made by connecting a voltmeter between
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The pGLO lab is a lab where students attempt to put the genes that make a jelly fish glow into E. Coli. After a process called transformation‚ the process in which a cell takes up and expresses a new piece of genetic information‚ the E. Coli will be able to glow and will be antibiotic resistant. The students first need to learn a couple of techniques before they are able to begin this lab. The first technique they will need is how to keep their environment sterile. They must learn to only open their
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