In Jane Horack’s article “Staphylococcus epidermidis”‚ S. epidermidis is described as “gram-positive cocci bacteria that are part of the normal flora on the skin and nasal passages.” The article goes on to say that the species was originally named Staphylococcus Albus by microbiologist Rosenback in 1884. When viewed under a microscope S. epidermidis will appear in chains‚ pairs‚ or grape-like clusters (Horak 1). Taxonomically‚ the species S. epidermidis falls in the genus Staphylococcus‚ which is
Premium Bacteria
In the experiment involving varying pH levels‚ E. faecalis‚ S. epidermidis‚ L. lactis‚ and L. casei were subjected to different pH levels and then were allowed to incubate in order to determine the minimum‚ maximum‚ and optimum pH levels for growth of specific bacterial species. It was found that lower pH levels between 2 and 4 inhibited or promoted little to no growth for E. faecalis‚ S. epidermidis‚ and L. casei. It is evident that these bacteria were resistant to alkaline environments‚ however
Premium Bacteria Escherichia coli Antibiotic resistance
extract was put in reagent bottles and kept refrigerated until use. E. Determination of the Activity of the Extract on the Chosen Bacteria The antibacterial property of leaves extracts obtained from Angelica keskei koidzumi was determined using Kirby-Bauer disk method. Using this method‚ a culture medium‚ nutrient agar‚ was uniformly and aseptically inoculated with the test organism and then filter paper discs soaked in 10 ug/mL of ashitaba extract are placed on the medium. Incubation was done for
Premium Bacteria
CHAPTER 4 RESULTS AND DISCUSSION 4.1. Angelika keiskei (Ashitaba )crude non-polar extract The crude non-polar extract from the plant Angelika keiskei (Ashitaba) was tested for the anti-bacterial property by using the Kirby – Bauer Disk Diffusion test. There were four 25uL of Ashitaba non-polar extract with different concentrations used which were 12.5 %‚ 25%‚ 50% and 100%. In line with this‚ respective positive controls of the two specimens and negative control were also used. The results
Premium Bacteria Escherichia coli Antibiotic resistance
Pure Culture Techniques In this first lab‚ you will be learning some very fundamental and important techniques. As is the case with most things‚ shorts cuts usually get you in trouble. This is especially true in Microbiology. The techniques you will be learning tonight‚ if mastered correctly‚ will make your life and learning experience in Microbiology much easier‚ if you don’t pay attention and practice these techniques incorrectly‚ well then……? Today you will be learning the following techniques:
Free Bacteria Staining Gram staining
generate a pure culture of a specific organism. Set Up: For this experiment I needed: 1 Distilled water‚ 1 Paper towels‚ 1 10%-bleach or 70% alcohol solution‚ 1 Zip bag‚ 1 Pan to heat agar‚ 1 Isopropyl alcohol (rubbing alcohol)‚ 1 Cultures: S. epidermidis and L. acidophilus‚ 1 Gloves‚ Disposable‚ 1 Pencil‚ marking‚ 11 Petri dish‚ 60 mm‚ 2 Candles (flame source)‚ 1 Thermometer-in-cardboard-tube‚6 Test Tube(6)‚ 16 x 125 mm in Bubble Bag‚ 1 Test tube holder‚ 1 Test-tube-rack-6x21-mm‚ 1 Pipet Graduated
Premium Yeast Petri dish Agar plate
analyzed was Staphylococcus epidermidis. This organism is gram-positive‚ single celled‚ arranged in grape-like clusters‚ and cocci in shape (Bukhari‚ 2004). S. epidermidis is approximately 0.5 to 1.5 micrometers in diameter‚ it is a facultative anaerobe but grows best in aerobic conditions (Bukhari‚ 2004). Typical hosts of this species are humans and other warm-blooded animals‚ where a favorable temperature of 37℃ is usually maintained in the intestinal tract. S. epidermidis may cause urinary tract
Premium Bacteria Growth medium Escherichia coli
The Antibacterial Effect of Papaya (Carica) Leaves Extract on Staphylococcus epidermidis Virgen Milagrosa University Foundation Special Science High School Martin P. Posadas Avenue‚ San Carlos City‚ Pangasinan Researcher S.Y. 2010-2011 The Antibacterial Effect of Papaya (Carica) Leaves Extract on Staphylococcus epidermidis A thesis Presented to the Faculty and staff of the Virgen Milagrosa University Foundation Special Science High School M.P. Posadas Ave.‚ San Carlos City
Premium Scientific method Scientific control
organisms‚ if any‚ fermented glucose? Both E. coli and S. epidermidis fermented glucose. G.Which of the organisms‚ if any‚ produced measurable acidic byproducts? Both E. coli and S. epidermidis produced measurable acidic byproduct. H.What is the cellular role of catalase? The cellular role of a catalase is to enable the breakdown of hydrogen peroxide into water and molecular oxygen. I.Which of the organisms‚ if any‚ produced catalase? S. epidermidis is the organism that produced catalase. J.Which of
Premium Metabolism Bacteria Oxygen
The ten Petri dishes that exclusively did not contain an antibiotic in the bacteria culture served as the control. There were ten trials for the control and each level of IV. The experiment began by cleaning the work area and sterilizing it with 70% ethanol. Then trypticase soy agar (TSA) was poured into six groups of 60 Petri dishes (See Appendix 1). The dishes were labeled based on the antibiotic used and were left to dry and solidify at room temperature. After an hour‚ the dishes were placed in
Premium Bacteria Petri dish Agar plate