1.7 Factors that affect the activity of an enzyme It is important when working with enzymes to understand basic enzymatic theory behind them when selecting conditions to measure the activity of the enzymes. The factors that are known to affect the concentration of enzymes are temperature‚ pH‚ concentration of enzyme‚ concentration of substrate‚ buffer type and concentration‚ the presence of any inhibitors and cofactors (Worthington-biochem.com). 1.7.1. Temperature With most catalysed reactions‚
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Title: The Effect of Varying Amounts of Substrate and Enzyme on a Reaction Rate Abstract In living organisms‚ certain reactions must take place rapidly to assist life. This occurs because of enzymes‚ because all reactions would take place too slowly to sustain life (Jacklet‚ 237). Enzymes are large protein molecules that catalyze specific chemical reactions without being used up in the process. Each enzyme has a region on its surface‚ called the active site‚ which recognizes a specific
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Abstract: After reviewing the basics of enzymes and catalysis‚ we take a dive into the wonderful world of catalase. Beginning with establishing a base line of just how much hydrogen peroxide there is in 5.0mL of the reacted solution; to figuring out exactly how much actually reacted after 300 seconds of catalyzed reaction. Follow the experiment from the beginning steps right to the end as you see where the students went wrong‚ interpretation of the results‚ and great answers to work sheet
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Kinetic study of a Solvolysis (Sn1) Reaction Aneadra Bowles Adasia Rutledge Krystal Flakes Robert Grimes Jasmine Ross June 5‚ 2012 Purpose: The purpose of this experiment is to use kinetics to study a solvolyis reaction Introduction: The purpose of this experiment is to understand the kinetics of the hydrolysis of t-butyl chloride.The kinetic order of reaction was studied under the effects of variations in temperature‚ solvent polarity‚ and structure. It is particularly observed in
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Potato Enzyme Lab INTRODUCTION An enzyme is a protein that speeds up or slows down a specific chemical reaction in an organism. A good rule of thumb is to remember that enzyme names end in “-ase”. This will help in identifying enzymes in further readings. Generally enzymes are catalysts. Hydrogen peroxide is a toxic chemical that is produced in many organisms during metabolism. Organisms must get rid of this toxin to survive. One reaction turns the hydrogen peroxide into water and oxygen. The
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Enzyme Lab Report Introduction The objective of this experiment was to determine if changes in pH or temperature affected the activity of enzymes‚ specifically the enzyme sucrase. Enzymes are protein molecules that act as biological catalysts to increase the speed of the reaction or to lower the activation energy of that reaction. However‚ the activity of an enzyme can be affected by physical factors such as pH and temperature because these factors alter the structure of the enzyme (Freeman‚ 2011)
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Type III Writing Assignment: Written in lab notebook 1. Results (recreate in notebook) 15 points/ _____ 2. Conclusion 20 points/ _____ 3. Questions 15 points/ _____ Lactase enzyme lab OBJECTIVES: 1. Understand how biological molecules are essential to the survival of living organisms * * 2. Compare the structures and functions of the major biological molecules(carbohydrates‚ proteins‚ lipids‚ and nucleic acids) as related to the survival
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Chemistry Unit 1 – Revision Questions Chemistry 1A 1) Define an element. How many are there in the Periodic Table? 2) Write a table with the charges and masses of protons‚ neutrons and electrons. 3) Define atomic number and mass number. 4) Draw electron (energy level) diagrams for Be‚ S‚ Al‚ Cl and K. Also‚ write down the number of protons and neutrons for each. Finally‚ write the symbols (with mass no. and atomic no.) 5) Why have all Group 7 elements (halogens) got similar chemical properties
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Potato Enzyme Lab Enzymes are protein catalysts that speed up chemical reaction in living organisms. The purpose of this lab was to; observe the breakdown of hydrogen peroxide toxin by potato’s enzyme catalase‚ determine factors that influence how quickly the reaction takes place‚ and determine factors that influence how well enzymes function. The first test‚ we were supposed to see what would happen when we add sand in hydrogen peroxide. We filled a test tube with 5mL hydrogen peroxide and we added
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Enzyme Lab Introduction/ Abstract An enzyme is a substance produced by a living organism that acts as a catalyst to bring about a specific biochemical reaction. They are mainly made up of proteins and can tremendously speed up reactions. E. coli ( a bacterium) has about 1‚000 different types of enzymes floating around in its cytoplasm at any given time. Enzymes can be used to join and even break up molecules as shown in the diagram below. (1)
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