Abstract: This experimentation was to evaluate absorbance and the reaction rate of an enzyme‚ ’-amylase in starch-iodine solution. We will be testing the relationship between enzymatic reaction affected by temperature and pH. Through the testing the enzyme at different temperatures‚ and different pH levels; it would determine at which temperature and pH level the enzyme worked the most efficiently. Analyzing absorbance of the solutions with spectrophotometery will determine the reaction rate. To
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Introduction: The purpose of this experiment is to measure the effects of changes in temperatures and pH on enzyme activity in skeletal muscle‚ particularly the activity of lactate dehydrogenase (LDH). LDH is a glycolytic enzyme which converts pyruvate to lactate in the following equation: LDH Pyruvate+ NADH ------------ Lactate + NAD The reaction above can move in both directions‚
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r Virtual Lab: Enzyme Controlled Reactions Instructions 1. Go to the following web-link in order to open the Virtual Lab: http://www.mhhe.com/biosci/genbio/virtual_labs_2K8/labs/BL_02/index.html 2. Open the Virtual Lab: Enzyme Controlled Reactions 3. The virtual lab simulation will be on the right side of the screen‚ and the “Question” column will be on the left side of the screen. 4. Click the monitor in the lab simulation to watch a video about enzyme action. 5. Click the “Information”
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Science IA Rough Draft The Effect of Temperature on The Activity of The Enzyme Catalase Introductions: Enzmyes are proteins that catalyze chemical reactions. All living things have catalase present in them. Catalase is a common HYPERLINK "http://en.wikipedia.org/wiki/Enzyme" enzyme found in nearly all living organisms that are exposed to oxygen‚ where it HYPERLINK "http://en.wikipedia.org/wiki/Catalyst" catalyzes the decomposition of HYPERLINK "http://en.wikipedia.org/wiki/Hydrogen_peroxide"
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Enzymes are catalytic proteins that selectively speed up chemical reactions without by consumed by the reaction itself (1). Enzyme activity is significantly affected by factors such as temperature‚ substrate concentration‚ enzyme concentration and pH. Enzymes are highly specific and only catalyse one specific chemical reaction‚ speeding up the reaction by lowering the activation energy‚ the energy required to start a chemical reaction (2). At high temperatures and with various mechanical forces or
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If the temperature is increased‚ the molecules will gain kinetic energy allowing them to move a lot more frequently. Enzymes begin a reaction when they randomly collide with the substrate molecule‚ this is where the enzyme will react upon. The speed and frequency of these collisions is dependent on the temperature‚ so an increase in temperature will effectively increase the rate of reaction and allow more products to be made. Consequently‚ an increase in the temperature will make the vibrational
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“Enzyme X is a highly pigmented protein that imparts the characteristic color to certain blue-green algae. It also facilitates a reaction necessary to the survival of this species; we can follow the kinetics of this reaction by measuring the conversion of Substance X to Substance Y at various times during purification.” Techniques and methodologies for extracting the proteins and purifying Enzyme X are as follows: The blue-green tinted proteins in Enzyme X give a distinct color to algae. The proteins
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1.7 Factors that affect the activity of an enzyme It is important when working with enzymes to understand basic enzymatic theory behind them when selecting conditions to measure the activity of the enzymes. The factors that are known to affect the concentration of enzymes are temperature‚ pH‚ concentration of enzyme‚ concentration of substrate‚ buffer type and concentration‚ the presence of any inhibitors and cofactors (Worthington-biochem.com). 1.7.1. Temperature With most catalysed reactions‚
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on Enzyme Activity Rate Abstract The reaction rate of an enzyme can be affected by many factors‚ and the purpose of this experiment was to find out how an increasing substrate concentration influences the rate of an enzyme activity; we obtained data from recording the absorbance of the samples which contain the same amount of potato juice (enzyme oxidase) and different amount of catechol (substrate) while holding pH and temperature constant. Our findings illustrate that the rate of enzyme activity
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Environment Can Effect Enzymes Introduction: In a chemical reaction there sometimes can be a catalyst present known as an enzyme. An enzyme is a protein that speeds up a chemical reaction by lowering the amount of activation energy required to start the reaction. By lowering the activation energy‚ more of the substrate is able to participate in the reaction‚ speeding it up. Enzymes are substrate specific. The substrate is what the enzyme bonds to. That is to say that enzyme A will only react with
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