BIO 5 Lab Report: Lactase Enzymes Enzymes are biological catalysts or assistants. Enzymes consist of various types of proteins that work to drive the chemical reaction required for a specific action or nutrient. Enzymes can either launch a reaction or speed it up. The chemicals that are transformed with the help of enzymes are called substrates. In the absence of enzymes‚ these chemicals are called reactants. Enzymes are thought to have an area with a very particular shape. When a molecule of
Premium Enzyme Chemical reaction Catalysis
20th‚ 2012 Enzyme Lab What is an enzyme? Enzymes are specialized protein molecules simplifying most of the body’s metabolic processes such as‚ supplying energy‚ digesting foods‚ purifying your blood‚ executing the body of waste products etc. Enzymes act as catalyst by speeding up the reactions that happen in our bodies and decreasing the amount of activation energy needed to break a complex down. A reactant is any given enzymatic reaction is called a substrate for that specific enzyme. The place
Free Enzyme PH
(SBI 4U1) Nicole Mikulis Unit: Biochemistry Sept 14 2012 Lab: Effect of temperature and pH on catalase activity BACKGROUND Catalase is an enzyme that detoxifies chemicals that might harm the cell such as hydrogen peroxide (H2O2). The enzyme breaks H2O2 into water and oxygen. The production of the oxygen gas bubbles serves as evidence that the catalase enzyme is working. As catalase is breaking the bonds between H2O2‚ it is releasing energy in the form of heat which
Premium PH Enzyme
of enzymes‚ components that influence the enzyme’s activity‚ identify an unknown phosphatase‚ influence of inhibitors‚ and determine if inhibition is competitive or noncompetitive. A spectrophotometer evaluated the measurement of color change over a period time due to product being formed. Determining unknown phosphatase and effects from different inhibitors were determined by varying the pH and substrate concentrations. The unknown phosphatase analyzed showed it was an acidic phosphatase. Ph 5 had
Premium
Lab #5-Enzymes NAME DATE LAB PERIOD Introduction Enzymes are proteins‚ though highly complex and diverse‚ they serve one basic function; to work as an organic catalyst. A catalyst‚ as defined by Merriam-Webster dictionary‚ is a substance that enables a chemical reaction to proceed at a usually faster rate ("Catalyst-Definition and more."). They function by reducing the activation energy‚ or energy required to start a reaction. The way enzymatic reaction works cannot be altered‚ but the
Premium Enzyme
LAB: Substrate Concentration Affecting the Rate of Enzyme Activity: Through the Experiment of Beef Liver Puree and Hydrogen Peroxide Research Question Does different amount of substrate affect the rate of enzyme activities? Purpose To examine how different types of concentration (Hydrogen Peroxide) affect the rate of enzyme activity. Hypothesis We believe that if there is more substrate concentrated‚ then there will be an increase in the rate of enzyme activity. This is because
Premium Enzyme Oxygen Hydrogen peroxide
Enzymes are catalytic proteins which speed up the rate of reactions. Every enzyme has a specific function – meaning‚ they can only bind to certain substrates. Because these enzymes are proteins‚ they can be denatured. Enzymes can be denatured by many factors‚ such as pH and temperature. This lab was divided into three parts which examined the effects of pH‚ enzyme concentration and temperature on the rate of which enzymes catalyze. The pH is an index of hydrogen ions. In acidic conditions‚ where
Premium Enzyme Chemical reaction Catalysis
Lab on pH Levels Purpose: Throughout this experiment we are trying to learn how to take proper pH measurements using a pH meter. We also want to see the pH of different common liquids and record the change in pH when we add acids and alkaline liquids. The last reason we are conducting this experiment is to explain and show how fresh milk spoils by the change in pH. Materials: In this experiment we used graduated cylinders and glass beakers and added variety of ingredients including 50 mL of; soda
Premium Chemistry Water Test method
LABORATORY REPORT (Click on the Save a Copy button on the panel above to save your report) Activity: Enzyme Activity Predictions 1. Sucrase will have the greatest activity at pH 6 2. Sucrase will have the greatest activity at 60 °C (140 °F) 3. Sucrase activity decreases with increasing sucrose concentration. Materials and Methods Effect of pH on Enzyme Activity. 1. Dependent Variable. amount of product (glucose and fructose) produced 2. Independent Variable. pH 3. Controlled Variables
Premium Enzyme PH Blood
Factors Affecting Enzyme Activity Abstract: In the following lab factors affecting enzyme activity‚ temperature‚ pH‚ enzyme concentration‚ substrate concentration and surface area will be tested on a beef liver enzyme to see if there will be any effect of performance. By doing 2 or more trials the results will show whether there is an effect to the enzyme from the following factors or not. Some of the factors may denature the enzyme and some will do nothing. Using a table qualitative and the
Premium Enzyme Chemical reaction Chemical equilibrium